Gene description for FKBP1A
Gene name FK506 binding protein 1A, 12kDa
Gene symbol FKBP1A
Other names/aliases FKBP-12
FKBP-1A
FKBP1
FKBP12
PKC12
PKCI2
PPIASE
Species Homo sapiens
 Database cross references - FKBP1A
ExoCarta ExoCarta_2280
Entrez Gene 2280
HGNC 3711
MIM 186945
UniProt P62942  
 FKBP1A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Melanoma cells 25950383    
Melanoma cells 25950383    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for FKBP1A
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 TAS
    activin binding GO:0048185 IPI
    ion channel binding GO:0044325 TAS
    signal transducer activity GO:0004871 IMP
    transforming growth factor beta receptor binding GO:0005160 TAS
    protein binding GO:0005515 IPI
    type I transforming growth factor beta receptor binding GO:0034713 ISS
    FK506 binding GO:0005528 NAS
    SMAD binding GO:0046332 IPI
    calcium channel inhibitor activity GO:0019855 IDA
    macrolide binding GO:0005527 NAS
Biological Process
    amyloid fibril formation GO:1990000 IDA
    ventricular cardiac muscle tissue morphogenesis GO:0055010 ISS
    protein peptidyl-prolyl isomerization GO:0000413 IDA
    positive regulation of protein binding GO:0032092 IDA
    regulation of amyloid precursor protein catabolic process GO:1902991 IGI
    T cell activation GO:0042110 NAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    negative regulation of ryanodine-sensitive calcium-release channel activity GO:0060315 IDA
    negative regulation of release of sequestered calcium ion into cytosol GO:0051280 IDA
    calcium ion transmembrane transport GO:0070588 NAS
    regulation of immune response GO:0050776 IMP
    regulation of activin receptor signaling pathway GO:0032925 IDA
    negative regulation of protein phosphatase type 2B activity GO:0032513 IDA
    heart morphogenesis GO:0003007 ISS
    positive regulation of protein ubiquitination GO:0031398 IDA
    regulation of ryanodine-sensitive calcium-release channel activity GO:0060314 ISS
    protein maturation by protein folding GO:0022417 TAS
    SMAD protein complex assembly GO:0007183 IDA
    chaperone-mediated protein folding GO:0061077 IBA
    regulation of protein localization GO:0032880 IGI
    protein refolding GO:0042026 TAS
    heart trabecula formation GO:0060347 ISS
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    extracellular fibril organization GO:0043206 IDA
    protein folding GO:0006457 NAS
    'de novo' protein folding GO:0006458 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular matrix GO:0031012 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    terminal cisterna GO:0014802 ISS
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IDA
    Z disc GO:0030018 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified FKBP1A in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
8
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
9
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FKBP1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITPR1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 Mtor 56718
Affinity Capture-Western Rattus norvegicus
3 RYR3 6263
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 MTOR  
Reconstituted Complex Mus musculus
5 RYR1 6261
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 PPP3R1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 DHFR 1719
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
9 FKBP4 2288
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 YY1 7528
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 TGFB1I1  
Affinity Capture-MS Homo sapiens
12 MTOR 2475
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
13 FKBP1A 2280
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
14 GLMN 11146
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
15 PPP3CA 5530
Affinity Capture-MS Homo sapiens
16 TGFBR1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here