Gene description for RPA1
Gene name replication protein A1, 70kDa
Gene symbol RPA1
Other names/aliases HSSB
MST075
REPA1
RF-A
RP-A
RPA70
Species Homo sapiens
 Database cross references - RPA1
ExoCarta ExoCarta_6117
Entrez Gene 6117
HGNC 10289
MIM 179835
UniProt P27694  
 RPA1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for RPA1
Molecular Function
    damaged DNA binding GO:0003684 IDA
    metal ion binding GO:0046872 IEA
    single-stranded DNA binding GO:0003697 IDA
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
Biological Process
    post-translational protein modification GO:0043687 TAS
    nucleotide-excision repair, DNA damage removal GO:0000718 TAS
    in utero embryonic development GO:0001701 IEA
    DNA damage response, detection of DNA damage GO:0042769 TAS
    DNA repair GO:0006281 TAS
    DNA-dependent DNA replication GO:0006261 TAS
    protein sumoylation GO:0016925 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    mitotic cell cycle GO:0000278 TAS
    base-excision repair GO:0006284 TAS
    double-strand break repair via homologous recombination GO:0000724 TAS
    error-free translesion synthesis GO:0070987 TAS
    hemopoiesis GO:0030097 IEA
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    meiotic nuclear division GO:0007126 IEA
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    cellular response to heat GO:0034605 TAS
    DNA recombination GO:0006310 TAS
    telomere maintenance via recombination GO:0000722 TAS
    regulation of cellular response to heat GO:1900034 TAS
    DNA recombinase assembly GO:0000730 TAS
    telomere maintenance GO:0000723 TAS
    mismatch repair GO:0006298 TAS
    cellular protein metabolic process GO:0044267 TAS
    double-strand break repair GO:0006302 TAS
    nucleotide-excision repair GO:0006289 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    error-prone translesion synthesis GO:0042276 TAS
    homeostasis of number of cells within a tissue GO:0048873 IEA
    DNA replication GO:0006260 TAS
    cell cycle checkpoint GO:0000075 IMP
    translesion synthesis GO:0019985 TAS
    positive regulation of cell proliferation GO:0008284 IEA
Subcellular Localization
    PML body GO:0016605 IDA
    DNA replication factor A complex GO:0005662 IPI
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    lateral element GO:0000800 IEA
    male germ cell nucleus GO:0001673 IEA
 Experiment description of studies that identified RPA1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TP53BP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 ATR  
Protein-peptide Homo sapiens
3 MCM7 4176
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 C8orf30A  
Two-hybrid Homo sapiens
5 FLJ20516  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
6 RPA3  
Invitro Homo sapiens
7 ORC2L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 ASCC2  
Two-hybrid Homo sapiens
9 KIN  
Co-fractionation Homo sapiens
10 RPA2 6118
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
11 RPA1 6117
Invitro Homo sapiens
Invitro Homo sapiens
12 FTS  
Two-hybrid Homo sapiens
13 XPA  
Invivo Homo sapiens
Invitro Homo sapiens
14 CPE  
Two-hybrid Homo sapiens
15 MCM4 4173
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 HELB  
Invitro Homo sapiens
17 TCEA2  
Two-hybrid Homo sapiens
18 PRKDC 5591
Reconstituted Complex Homo sapiens
19 BLM 641
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
20 MUTYH  
Invivo Homo sapiens
21 MSH4  
Invivo Homo sapiens
Invitro Homo sapiens
22 RBM23  
Two-hybrid Homo sapiens
23 FANCA  
Affinity Capture-MS Homo sapiens
24 BRCA2 675
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 SELENBP1 8991
Two-hybrid Homo sapiens
26 MCM2 4171
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 TP53  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 RPA4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
29 ATRIP  
Co-localization Homo sapiens
30 PCNA 5111
Invitro Homo sapiens
31 MCM6 4175
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 ORC6L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 RAD51  
Invitro Homo sapiens
Invivo Homo sapiens
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