Gene description for ACACA
Gene name acetyl-CoA carboxylase alpha
Gene symbol ACACA
Other names/aliases ACAC
ACACAD
ACC
ACC1
ACCA
Species Homo sapiens
 Database cross references - ACACA
ExoCarta ExoCarta_31
Vesiclepedia VP_31
Entrez Gene 31
HGNC 84
MIM 200350
UniProt Q13085  
 ACACA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for ACACA
Molecular Function
    acetyl-CoA carboxylase activity GO:0003989 IBA
    acetyl-CoA carboxylase activity GO:0003989 IDA
    acetyl-CoA carboxylase activity GO:0003989 ISS
    acetyl-CoA carboxylase activity GO:0003989 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    tissue homeostasis GO:0001894 IEA
    acetyl-CoA metabolic process GO:0006084 ISS
    fatty acid biosynthetic process GO:0006633 IBA
    fatty acid biosynthetic process GO:0006633 IC
    fatty acid biosynthetic process GO:0006633 ISS
    fatty-acyl-CoA biosynthetic process GO:0046949 TAS
    protein homotetramerization GO:0051289 ISS
    lipid homeostasis GO:0055088 IEA
    cellular response to prostaglandin E stimulus GO:0071380 IEA
    malonyl-CoA biosynthetic process GO:2001295 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    actin cytoskeleton GO:0015629 IDA
 Experiment description of studies that identified ACACA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
15
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
26
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
35
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
40
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACACA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 ADRBK1 156
Affinity Capture-MS Homo sapiens
3 AKR1B10 57016
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 PPHLN1  
Negative Genetic Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 MCM7 4176
Co-fractionation Homo sapiens
7 UBE2H 7328
Affinity Capture-MS Homo sapiens
8 PRKAA1 5562
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
9 HDAC4  
Affinity Capture-MS Homo sapiens
10 HOOK1  
Proximity Label-MS Homo sapiens
11 STK25 10494
Affinity Capture-MS Homo sapiens
12 NOP58 51602
Negative Genetic Homo sapiens
13 CCT3 7203
Co-fractionation Homo sapiens
14 BCL2  
Positive Genetic Homo sapiens
15 SLFN11 91607
Positive Genetic Homo sapiens
16 SHC1 6464
Affinity Capture-MS Homo sapiens
17 MACROD1 28992
Positive Genetic Homo sapiens
18 AP2S1 1175
Negative Genetic Homo sapiens
19 KIAA1211  
Positive Genetic Homo sapiens
20 KLF9 687
Positive Genetic Homo sapiens
21 C3orf58 205428
Negative Genetic Homo sapiens
22 HACD3 51495
Negative Genetic Homo sapiens
23 PSMD11 5717
Co-fractionation Homo sapiens
24 RBM34  
Positive Genetic Homo sapiens
25 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MID1IP1  
Affinity Capture-MS Homo sapiens
27 SERPINB6 5269
Negative Genetic Homo sapiens
28 BRCA1 672
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
29 CENPN  
Positive Genetic Homo sapiens
30 SEPT5 5413
Negative Genetic Homo sapiens
31 MYO10 4651
Negative Genetic Homo sapiens
32 MGEA5 10724
Positive Genetic Homo sapiens
33 BRD2  
Negative Genetic Homo sapiens
34 MAP4K4 9448
Negative Genetic Homo sapiens
35 C4orf26  
Affinity Capture-MS Homo sapiens
36 RANBP3 8498
Positive Genetic Homo sapiens
37 NDUFA4 4697
Affinity Capture-MS Homo sapiens
38 UNKL  
Negative Genetic Homo sapiens
39 C17orf96  
Positive Genetic Homo sapiens
40 C15orf48  
Affinity Capture-MS Homo sapiens
41 ARMC5 79798
Positive Genetic Homo sapiens
42 SBDS 51119
Positive Genetic Homo sapiens
43 SLC12A4 6560
Positive Genetic Homo sapiens
44 UBE2G2  
Negative Genetic Homo sapiens
45 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
46 KIAA1429 25962
Affinity Capture-MS Homo sapiens
47 RNH1 6050
Co-fractionation Homo sapiens
48 PSMD2 5708
Co-fractionation Homo sapiens
49 SIAH3  
Positive Genetic Homo sapiens
50 HDAC3 8841
Negative Genetic Homo sapiens
51 PPP2R5D 5528
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 PCNT  
Negative Genetic Homo sapiens
53 PC 5091
Affinity Capture-MS Homo sapiens
54 RBM39 9584
Negative Genetic Homo sapiens
55 TGFB1 7040
Affinity Capture-MS Homo sapiens
56 COQ9  
Affinity Capture-MS Homo sapiens
57 MATR3 9782
Co-fractionation Homo sapiens
58 DCTN4 51164
Proximity Label-MS Homo sapiens
59 SPRTN  
Affinity Capture-MS Homo sapiens
60 GNS 2799
Co-fractionation Homo sapiens
61 CCT6A 908
Positive Genetic Homo sapiens
62 KIF5B 3799
Co-fractionation Homo sapiens
63 SPAG9 9043
Co-fractionation Homo sapiens
64 RUNDC1  
Positive Genetic Homo sapiens
65 MEIS3  
Positive Genetic Homo sapiens
66 NOL4L  
Negative Genetic Homo sapiens
67 NPM1 4869
Affinity Capture-MS Homo sapiens
68 PROCR 10544
Positive Genetic Homo sapiens
69 MARS 4141
Positive Genetic Homo sapiens
70 ELOF1  
Positive Genetic Homo sapiens
71 PSMC6 5706
Co-fractionation Homo sapiens
72 DDX58 23586
Affinity Capture-RNA Homo sapiens
73 PSMC2 5701
Co-fractionation Homo sapiens
74 RAD51  
Positive Genetic Homo sapiens
75 CCNL2 81669
Positive Genetic Homo sapiens
76 THRSP  
Affinity Capture-MS Homo sapiens
77 PIGC 5279
Negative Genetic Homo sapiens
78 PGRMC2 10424
Negative Genetic Homo sapiens
79 CHGB 1114
Two-hybrid Homo sapiens
80 SMC5 23137
Positive Genetic Homo sapiens
81 VDAC1 7416
Affinity Capture-MS Homo sapiens
82 SLX4  
Affinity Capture-MS Homo sapiens
83 C6orf57  
Negative Genetic Homo sapiens
84 ELMO2 63916
Negative Genetic Homo sapiens
85 DDX6 1656
Positive Genetic Homo sapiens
86 FAM192A  
Positive Genetic Homo sapiens
87 DHODH 1723
Negative Genetic Homo sapiens
88 FPGS 2356
Negative Genetic Homo sapiens
89 KNSTRN  
Negative Genetic Homo sapiens
90 MLLT1 4298
Affinity Capture-MS Homo sapiens
91 RHOG 391
Negative Genetic Homo sapiens
92 SREK1 140890
Positive Genetic Homo sapiens
93 PSMC3 5702
Co-fractionation Homo sapiens
94 DVL2 1856
Affinity Capture-MS Homo sapiens
95 DYNLT1 6993
Proximity Label-MS Homo sapiens
96 ERCC6L 54821
Co-fractionation Homo sapiens
97 HSPA8 3312
Co-fractionation Homo sapiens
98 SMC2 10592
Co-fractionation Homo sapiens
99 OXSM  
Negative Genetic Homo sapiens
100 TMEM25  
Negative Genetic Homo sapiens
101 AURKA 6790
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
102 STRA13  
Positive Genetic Homo sapiens
103 ATP5A1 498
Positive Genetic Homo sapiens
104 COLEC10 10584
Positive Genetic Homo sapiens
105 TBCE 6905
Positive Genetic Homo sapiens
106 MRPL35  
Negative Genetic Homo sapiens
107 NFRKB  
Positive Genetic Homo sapiens
108 KIF2B 84643
Positive Genetic Homo sapiens
109 PRELID1  
Positive Genetic Homo sapiens
110 TGM2 7052
Co-fractionation Homo sapiens
111 PARK2  
Affinity Capture-MS Homo sapiens
112 OAS2 4939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 RAB15 376267
Negative Genetic Homo sapiens
114 SNRPA 6626
Co-fractionation Homo sapiens
115 FAM186B  
Negative Genetic Homo sapiens
116 SMEK1 55671
Co-fractionation Homo sapiens
117 IL25  
Positive Genetic Homo sapiens
118 PRKAG1 5571
Biochemical Activity Homo sapiens
119 CBL 867
Affinity Capture-MS Homo sapiens
120 SOAT1 6646
Negative Genetic Homo sapiens
121 COPB1 1315
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
122 SOCS6  
Affinity Capture-MS Homo sapiens
123 CDC42 998
Positive Genetic Homo sapiens
124 ACACA 31
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
125 SUPT16H 11198
Negative Genetic Homo sapiens
126 C12orf49  
Negative Genetic Homo sapiens
Negative Genetic Homo sapiens
127 UBASH3B 84959
Affinity Capture-MS Homo sapiens
128 NDUFAF5  
Positive Genetic Homo sapiens
129 CHMP4B 128866
Affinity Capture-MS Homo sapiens
130 SREBF2 6721
Negative Genetic Homo sapiens
131 RPA2 6118
Proximity Label-MS Homo sapiens
132 MYH9 4627
Co-fractionation Homo sapiens
133 VCP 7415
Affinity Capture-MS Homo sapiens
134 DDX5 1655
Positive Genetic Homo sapiens
135 NTRK1 4914
Affinity Capture-MS Homo sapiens
136 PATL1 219988
Positive Genetic Homo sapiens
137 EFNA3  
Affinity Capture-MS Homo sapiens
138 C20orf195  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 CENPM  
Positive Genetic Homo sapiens
140 DTX2 113878
Proximity Label-MS Homo sapiens
141 PPP2CA 5515
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
142 MOGS 7841
Negative Genetic Homo sapiens
143 PIGR 5284
Affinity Capture-MS Homo sapiens
144 SRSF1 6426
Negative Genetic Homo sapiens
145 ENO1 2023
Affinity Capture-RNA Homo sapiens
146 CSNK1E 1454
Affinity Capture-MS Homo sapiens
147 MYH10 4628
Co-fractionation Homo sapiens
148 CYB561D2 11068
Positive Genetic Homo sapiens
149 VMA21  
Negative Genetic Homo sapiens
150 HECTD1 25831
Positive Genetic Homo sapiens
151 KCMF1 56888
Positive Genetic Homo sapiens
152 PCCA 5095
Affinity Capture-MS Homo sapiens
153 IARS 3376
Co-fractionation Homo sapiens
154 MBD3 53615
Positive Genetic Homo sapiens
155 TRMT2A 27037
Positive Genetic Homo sapiens
156 MCCC1 56922
Affinity Capture-MS Homo sapiens
157 PRKAB1 5564
Biochemical Activity Homo sapiens
158 INTS6 26512
Positive Genetic Homo sapiens
159 VDAC2 7417
Negative Genetic Homo sapiens
160 UBA1 7317
Positive Genetic Homo sapiens
161 MCM2 4171
Co-fractionation Homo sapiens
162 SLC33A1 9197
Negative Genetic Homo sapiens
163 SLC12A9 56996
Positive Genetic Homo sapiens
164 NEIL3  
Protein-RNA Homo sapiens
165 HIST1H2BJ 8970
Affinity Capture-MS Homo sapiens
166 RPS6KA1 6195
Affinity Capture-MS Homo sapiens
167 TRIM16 10626
Positive Genetic Homo sapiens
168 CA9 768
Affinity Capture-MS Homo sapiens
169 ZC3H18  
Positive Genetic Homo sapiens
170 ATXN1 6310
Two-hybrid Homo sapiens
171 KAT7  
Negative Genetic Homo sapiens
172 RAB18 22931
Positive Genetic Homo sapiens
173 ECH1 1891
Affinity Capture-MS Homo sapiens
174 UBE2N 7334
Affinity Capture-MS Homo sapiens
175 EPB41L1 2036
Co-fractionation Homo sapiens
176 PER2  
Proximity Label-MS Homo sapiens
177 PSMA3 5684
Co-fractionation Homo sapiens
178 DNAJA1 3301
Positive Genetic Homo sapiens
179 COX7A2 1347
Affinity Capture-MS Homo sapiens
180 MRPL21 219927
Positive Genetic Homo sapiens
181 INPP5A 3632
Negative Genetic Homo sapiens
182 AIFM1 9131
Positive Genetic Homo sapiens
183 CDC73  
Affinity Capture-MS Homo sapiens
184 KCTD1 284252
Positive Genetic Homo sapiens
185 FGF14  
Two-hybrid Homo sapiens
186 MCCC2 64087
Affinity Capture-MS Homo sapiens
187 TIPRL 261726
Negative Genetic Homo sapiens
188 XPO1 7514
Positive Genetic Homo sapiens
189 EGLN3  
Affinity Capture-MS Homo sapiens
190 PCCB 5096
Affinity Capture-MS Homo sapiens
191 SIRT7  
Affinity Capture-MS Homo sapiens
192 WEE1 7465
Negative Genetic Homo sapiens
193 RLIM 51132
Negative Genetic Homo sapiens
194 PRKAA2 5563
Affinity Capture-MS Homo sapiens
195 PRKAR2A 5576
Co-fractionation Homo sapiens
196 PTPRA 5786
Positive Genetic Homo sapiens
197 CD274 29126
Affinity Capture-MS Homo sapiens
198 MAFF 23764
Positive Genetic Homo sapiens
199 HSD3B7 80270
Positive Genetic Homo sapiens
200 CUL3 8452
Affinity Capture-MS Homo sapiens
201 CLCC1 23155
Negative Genetic Homo sapiens
202 CKAP2  
Affinity Capture-MS Homo sapiens
203 ARIH2 10425
Positive Genetic Homo sapiens
204 EGFR 1956
Affinity Capture-MS Homo sapiens
205 HOOK3 84376
Proximity Label-MS Homo sapiens
206 PHKG2 5261
Affinity Capture-MS Homo sapiens
207 FBXO6 26270
Affinity Capture-MS Homo sapiens
208 FUS 2521
Affinity Capture-MS Homo sapiens
209 ACE2 59272
Proximity Label-MS Homo sapiens
210 PMPCB 9512
Positive Genetic Homo sapiens
211 SUZ12  
Affinity Capture-MS Homo sapiens
212 SART1 9092
Co-fractionation Homo sapiens
213 FN1 2335
Affinity Capture-MS Homo sapiens
214 MSTO1 55154
Negative Genetic Homo sapiens
215 NXF1 10482
Affinity Capture-RNA Homo sapiens
Negative Genetic Homo sapiens
216 AP2M1 1173
Affinity Capture-MS Homo sapiens
217 PRKCI 5584
Co-fractionation Homo sapiens
218 SLC35B2 347734
Negative Genetic Homo sapiens
219 PRMT5 10419
Co-fractionation Homo sapiens
220 DCAF6 55827
Co-fractionation Homo sapiens
221 CORO2B 10391
Negative Genetic Homo sapiens
222 EPHA2 1969
Proximity Label-MS Homo sapiens
223 ARHGAP25  
Affinity Capture-MS Homo sapiens
224 GANAB 23193
Negative Genetic Homo sapiens
225 INTS1 26173
Positive Genetic Homo sapiens
226 IFIT1 3434
Affinity Capture-MS Homo sapiens
227 RPA3 6119
Proximity Label-MS Homo sapiens
228 SEC31A 22872
Negative Genetic Homo sapiens
229 MBTD1  
Negative Genetic Homo sapiens
230 ATP1B3 483
Affinity Capture-MS Homo sapiens
231 PGAP2  
Negative Genetic Homo sapiens
232 SOD2 6648
Positive Genetic Homo sapiens
233 SART3 9733
Positive Genetic Homo sapiens
234 NUP214 8021
Positive Genetic Homo sapiens
235 BICD1 636
Proximity Label-MS Homo sapiens
236 PIGF  
Affinity Capture-MS Homo sapiens
237 RPL18 6141
Positive Genetic Homo sapiens
238 RNF2  
Affinity Capture-MS Homo sapiens
239 BMI1  
Affinity Capture-MS Homo sapiens
240 ILK 3611
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
241 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
242 MRPS12  
Positive Genetic Homo sapiens
243 HSPD1 3329
Co-fractionation Homo sapiens
244 ESF1 51575
Positive Genetic Homo sapiens
245 CCDC140  
Negative Genetic Homo sapiens
246 CCDC125 202243
Positive Genetic Homo sapiens
247 EEF2K  
Affinity Capture-MS Homo sapiens
248 C9orf114  
Positive Genetic Homo sapiens
249 HFE2  
Negative Genetic Homo sapiens
250 MDM2  
Affinity Capture-RNA Homo sapiens
251 YRDC 79693
Negative Genetic Homo sapiens
252 STK3 6788
Affinity Capture-MS Homo sapiens
253 CCDC71  
Affinity Capture-MS Homo sapiens
254 C5orf46 389336
Positive Genetic Homo sapiens
255 H2AFX 3014
Affinity Capture-MS Homo sapiens
256 PFAS 5198
Negative Genetic Homo sapiens
257 QRICH1  
Positive Genetic Homo sapiens
258 ZRANB1 54764
Affinity Capture-MS Homo sapiens
259 DDX46 9879
Negative Genetic Homo sapiens
260 PSMB2 5690
Co-fractionation Homo sapiens
261 BFAR  
Positive Genetic Homo sapiens
262 IKBKAP 8518
Affinity Capture-MS Homo sapiens
263 ELL  
Positive Genetic Homo sapiens
264 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
265 FASN 2194
Negative Genetic Homo sapiens
266 CLSPN  
Positive Genetic Homo sapiens
267 NUP205 23165
Positive Genetic Homo sapiens
268 CTR9 9646
Affinity Capture-MS Homo sapiens
269 SIRT6  
Affinity Capture-MS Homo sapiens
270 PSG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
271 SLC25A28  
Positive Genetic Homo sapiens
272 PPIL2  
Positive Genetic Homo sapiens
273 RAB3GAP2 25782
Negative Genetic Homo sapiens
274 LIPH 200879
Affinity Capture-MS Homo sapiens
275 PGRMC1 10857
Affinity Capture-MS Homo sapiens
276 INPPL1 3636
Affinity Capture-MS Homo sapiens
277 HCCS 3052
Positive Genetic Homo sapiens
278 ZNF746  
Affinity Capture-MS Homo sapiens
279 ATL2 64225
Negative Genetic Homo sapiens
280 ITGB1 3688
Negative Genetic Homo sapiens
281 LRPPRC 10128
Co-fractionation Homo sapiens
282 IER3IP1 51124
Positive Genetic Homo sapiens
283 GMPS 8833
Negative Genetic Homo sapiens
284 HNRNPA2B1 3181
Negative Genetic Homo sapiens
285 APOC1  
Two-hybrid Homo sapiens
286 AURKC  
Positive Genetic Homo sapiens
287 RALGAPB 57148
Negative Genetic Homo sapiens
288 TSEN2  
Positive Genetic Homo sapiens
289 ANAPC1 64682
Positive Genetic Homo sapiens
290 Shoc2  
Affinity Capture-MS Mus musculus
291 AEN  
Positive Genetic Homo sapiens
292 LSS 4047
Positive Genetic Homo sapiens
293 ELMOD3  
Positive Genetic Homo sapiens
294 SHMT2 6472
Negative Genetic Homo sapiens
295 SIRT1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
296 ARF4 378
Proximity Label-MS Homo sapiens
297 KPNA3 3839
Negative Genetic Homo sapiens
298 MRPS21  
Negative Genetic Homo sapiens
299 RPL35 11224
Positive Genetic Homo sapiens
300 PSMA1 5682
Co-fractionation Homo sapiens
301 UFL1 23376
Negative Genetic Homo sapiens
302 KATNA1 11104
Positive Genetic Homo sapiens
303 NPTXR 23467
Affinity Capture-MS Homo sapiens
304 NUP50 10762
Co-fractionation Homo sapiens
305 PAF1 54623
Affinity Capture-MS Homo sapiens
306 SPAG7  
Negative Genetic Homo sapiens
307 RIOK2 55781
Affinity Capture-MS Homo sapiens
308 MOV10 4343
Affinity Capture-RNA Homo sapiens
309 RBSN 64145
Negative Genetic Homo sapiens
310 PSMA5 5686
Co-fractionation Homo sapiens
311 EIF3M 10480
Negative Genetic Homo sapiens
312 H2AFZ 3015
Negative Genetic Homo sapiens
313 ARRB1 408
Affinity Capture-MS Homo sapiens
314 CDK9 1025
Affinity Capture-MS Homo sapiens
315 PINK1  
Affinity Capture-MS Homo sapiens
316 PRDX1 5052
Positive Genetic Homo sapiens
317 COX5A 9377
Affinity Capture-MS Homo sapiens
318 DHX38 9785
Positive Genetic Homo sapiens
319 PSMB7 5695
Co-fractionation Homo sapiens
320 ACACB 32
Affinity Capture-MS Homo sapiens
321 MLLT3 4300
Affinity Capture-MS Homo sapiens
322 KCTD17 79734
Positive Genetic Homo sapiens
323 PIK3R2 5296
Affinity Capture-MS Homo sapiens
324 SGCZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
325 KERA  
Positive Genetic Homo sapiens
326 C9orf72  
Affinity Capture-MS Homo sapiens
327 PPP2R1A 5518
Co-fractionation Homo sapiens
328 ATXN3 4287
Affinity Capture-MS Homo sapiens
329 CDK6 1021
Positive Genetic Homo sapiens
330 PSMC4 5704
Co-fractionation Homo sapiens
331 REC8  
Positive Genetic Homo sapiens
332 MRPS9 64965
Positive Genetic Homo sapiens
333 ZDBF2  
Negative Genetic Homo sapiens
334 EP300 2033
Affinity Capture-MS Homo sapiens
335 SLCO4A1 28231
Negative Genetic Homo sapiens
336 REEP4  
Positive Genetic Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here