Gene description for CDK6
Gene name cyclin-dependent kinase 6
Gene symbol CDK6
Other names/aliases MCPH12
PLSTIRE
Species Homo sapiens
 Database cross references - CDK6
ExoCarta ExoCarta_1021
Entrez Gene 1021
HGNC 1777
MIM 603368
UniProt Q00534  
 CDK6 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for CDK6
Molecular Function
    ATP binding GO:0005524 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IDA
    cyclin binding GO:0030332 IPI
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of cell differentiation GO:0045596 TAS
    T cell differentiation in thymus GO:0033077 IEA
    regulation of cell motility GO:2000145 ISS
    negative regulation of osteoblast differentiation GO:0045668 IDA
    cell cycle arrest GO:0007050 TAS
    positive regulation of gene expression GO:0010628 IEA
    gliogenesis GO:0042063 IMP
    negative regulation of cell cycle GO:0045786 IDA
    hematopoietic stem cell differentiation GO:0060218 IEA
    positive regulation of cell-matrix adhesion GO:0001954 IDA
    generation of neurons GO:0048699 ISS
    dentate gyrus development GO:0021542 ISS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    mitotic cell cycle GO:0000278 TAS
    positive regulation of fibroblast proliferation GO:0048146 IMP
    negative regulation of myeloid cell differentiation GO:0045638 IDA
    Notch signaling pathway GO:0007219 IEA
    regulation of gene expression GO:0010468 IMP
    protein phosphorylation GO:0006468 IDA
    regulation of erythrocyte differentiation GO:0045646 IMP
    astrocyte development GO:0014002 ISS
    negative regulation of cell proliferation GO:0008285 TAS
    lateral ventricle development GO:0021670 ISS
    negative regulation of cellular senescence GO:2000773 IDA
    cell division GO:0051301 IEA
    cell dedifferentiation GO:0043697 IMP
    response to virus GO:0009615 IEP
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    type B pancreatic cell development GO:0003323 IDA
Subcellular Localization
    ruffle GO:0001726 IDA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    centrosome GO:0005813 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified CDK6 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
3
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for CDK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDKN2A  
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
2 PPP2CA 5515
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
3 PPM1B 5495
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
4 CCND1 595
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 PCNA 5111
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
6 CNOT7 29883
Affinity Capture-MS Homo sapiens
7 CDK2 1017
Co-purification Homo sapiens
8 MCM10 55388
Affinity Capture-MS Homo sapiens
9 CDKN2D  
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
10 CDKN1B 1027
Affinity Capture-Western Homo sapiens
11 CCND2  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
12 CDKN1A  
Affinity Capture-MS Homo sapiens
13 MCM2 4171
Affinity Capture-MS Homo sapiens
14 CDKN2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
15 CDK6 1021
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
16 CCND3  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
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