Gene description for CLPX
Gene name caseinolytic mitochondrial matrix peptidase chaperone subunit
Gene symbol CLPX
Other names/aliases -
Species Homo sapiens
 Database cross references - CLPX
ExoCarta ExoCarta_10845
Vesiclepedia VP_10845
Entrez Gene 10845
HGNC 2088
MIM 615611
UniProt O76031  
 CLPX identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CLPX
Molecular Function
    ATP-dependent peptidase activity GO:0004176 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 ISS
    zinc ion binding GO:0008270 IEA
    peptidase activator activity GO:0016504 IDA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IDA
    protein dimerization activity GO:0046983 IEA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IEA
    proteolysis GO:0006508 IDA
    ATP metabolic process GO:0046034 IDA
    proteolysis involved in protein catabolic process GO:0051603 IBA
    proteolysis involved in protein catabolic process GO:0051603 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 NAS
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
    endopeptidase Clp complex GO:0009368 IDA
    endopeptidase Clp complex GO:0009368 IPI
    mitochondrial endopeptidase Clp complex GO:0009841 IDA
    mitochondrial nucleoid GO:0042645 IDA
 Experiment description of studies that identified CLPX in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLPX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
3 PMPCA 23203
Proximity Label-MS Homo sapiens
4 MCM2 4171
Affinity Capture-MS Homo sapiens
5 MAGEA3  
Affinity Capture-MS Homo sapiens
6 DNAJA3 9093
Affinity Capture-MS Homo sapiens
7 OXLD1  
Affinity Capture-MS Homo sapiens
8 Sacm1l 83493
Affinity Capture-MS Mus musculus
9 Ybx1 22608
Affinity Capture-MS Mus musculus
10 EBNA-LP  
Affinity Capture-MS
11 KLF8  
Affinity Capture-MS Homo sapiens
12 MTG2  
Proximity Label-MS Homo sapiens
13 NGRN  
Proximity Label-MS Homo sapiens
14 CHCHD1  
Proximity Label-MS Homo sapiens
15 GPT2  
Affinity Capture-MS Homo sapiens
16 LOC100132735  
Protein-RNA Homo sapiens
17 RPUSD4 84881
Proximity Label-MS Homo sapiens
18 DNAJC15  
Proximity Label-MS Homo sapiens
19 MTRF1  
Proximity Label-MS Homo sapiens
20 CCDC90B  
Proximity Label-MS Homo sapiens
21 LRPPRC 10128
Proximity Label-MS Homo sapiens
22 ATPAF2 91647
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NDUFS3 4722
Affinity Capture-MS Homo sapiens
24 KIF23 9493
Affinity Capture-MS Homo sapiens
25 COQ9  
Affinity Capture-MS Homo sapiens
26 SDHB 6390
Affinity Capture-MS Homo sapiens
27 PTPMT1 114971
Affinity Capture-MS Homo sapiens
28 GNS 2799
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CHCHD2  
Affinity Capture-MS Homo sapiens
30 NIT1 4817
Affinity Capture-MS Homo sapiens
31 HIST2H3A 333932
Cross-Linking-MS (XL-MS) Homo sapiens
32 YARS2  
Affinity Capture-MS Homo sapiens
33 HINT2 84681
Proximity Label-MS Homo sapiens
34 CHCHD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 THTPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 FTSJ2 29960
Affinity Capture-MS Homo sapiens
37 C1qbp 12261
Affinity Capture-MS Mus musculus
38 GCAT  
Affinity Capture-MS Homo sapiens
39 MRPS26 64949
Proximity Label-MS Homo sapiens
40 C3orf62  
Proximity Label-MS Homo sapiens
41 CLEC3A  
Affinity Capture-MS Homo sapiens
42 EXD2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
43 NKX2-5  
Affinity Capture-MS Homo sapiens
44 TRMT1 55621
Affinity Capture-MS Homo sapiens
45 CUL1 8454
Affinity Capture-MS Homo sapiens
46 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PMPCB 9512
Proximity Label-MS Homo sapiens
48 TACO1  
Proximity Label-MS Homo sapiens
49 VWA8 23078
Proximity Label-MS Homo sapiens
50 MTERF3  
Proximity Label-MS Homo sapiens
51 KIF14 9928
Affinity Capture-MS Homo sapiens
52 DDX58 23586
Affinity Capture-RNA Homo sapiens
53 MDH2 4191
Proximity Label-MS Homo sapiens
54 KLF16  
Affinity Capture-MS Homo sapiens
55 ATP5A1 498
Affinity Capture-MS Homo sapiens
56 FASLG 356
Affinity Capture-MS Homo sapiens
57 C6orf203  
Proximity Label-MS Homo sapiens
58 FASTKD5  
Proximity Label-MS Homo sapiens
59 ZNF330  
Cross-Linking-MS (XL-MS) Homo sapiens
60 LSM14B 149986
Affinity Capture-MS Homo sapiens
61 C20orf24  
Affinity Capture-MS Homo sapiens
62 CUL4B 8450
Affinity Capture-MS Homo sapiens
63 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 HMGN2 3151
Co-fractionation Homo sapiens
65 AMACR 23600
Affinity Capture-MS Homo sapiens
66 TUFM 7284
Proximity Label-MS Homo sapiens
67 HSCB 150274
Proximity Label-MS Homo sapiens
68 NTRK1 4914
Affinity Capture-MS Homo sapiens
69 PDP2  
Affinity Capture-MS Homo sapiens
70 POLDIP2 26073
Affinity Capture-MS Homo sapiens
71 TBRG4 9238
Proximity Label-MS Homo sapiens
72 ARRDC3 57561
Affinity Capture-MS Homo sapiens
73 DDX54 79039
Affinity Capture-MS Homo sapiens
74 TRUB2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
75 MRPL11 65003
Proximity Label-MS Homo sapiens
76 MTRF1L  
Proximity Label-MS Homo sapiens
77 COX4I1 1327
Proximity Label-MS Homo sapiens
78 CISD3 284106
Affinity Capture-MS Homo sapiens
79 OGT 8473
Reconstituted Complex Homo sapiens
80 OTC 5009
Proximity Label-MS Homo sapiens
81 MRRF  
Proximity Label-MS Homo sapiens
82 AIFM1 9131
Proximity Label-MS Homo sapiens
83 MRPS12  
Proximity Label-MS Homo sapiens
84 MTIF2 4528
Proximity Label-MS Homo sapiens
85 HSPE1 3336
Co-fractionation Homo sapiens
86 MTIF3  
Proximity Label-MS Homo sapiens
87 KIF12 113220
Affinity Capture-MS Homo sapiens
88 METTL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CUL3 8452
Affinity Capture-MS Homo sapiens
90 RMND1 55005
Proximity Label-MS Homo sapiens
91 EGFR 1956
Affinity Capture-MS Homo sapiens
92 FAHD1 81889
Affinity Capture-MS Homo sapiens
93 FBXO6 26270
Affinity Capture-MS Homo sapiens
94 C17orf80 55028
Proximity Label-MS Homo sapiens
95 C12orf65  
Proximity Label-MS Homo sapiens
96 MAPK6  
Affinity Capture-MS Homo sapiens
97 Cep72  
Affinity Capture-MS Mus musculus
98 ACAD9 28976
Proximity Label-MS Homo sapiens
99 FOXRED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 RNF2  
Affinity Capture-MS Homo sapiens
101 DHRS3 9249
Affinity Capture-MS Homo sapiens
102 HSPA9 3313
Proximity Label-MS Homo sapiens
103 MCUR1 63933
Proximity Label-MS Homo sapiens
104 CLPP 8192
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
105 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
106 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
107 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 TSFM 10102
Proximity Label-MS Homo sapiens
109 AUH 549
Proximity Label-MS Homo sapiens
110 LIG3 3980
Affinity Capture-MS Homo sapiens
111 GRB2 2885
Affinity Capture-MS Homo sapiens
112 FBXL6  
Affinity Capture-MS Homo sapiens
113 COQ5  
Affinity Capture-MS Homo sapiens
114 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
115 LONP1 9361
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
116 C7orf55  
Affinity Capture-MS Homo sapiens
117 PDHA1 5160
Proximity Label-MS Homo sapiens
118 AGMAT  
Affinity Capture-MS Homo sapiens
119 COX8A  
Proximity Label-MS Homo sapiens
120 C21orf33  
Proximity Label-MS Homo sapiens
121 SSBP1 6742
Proximity Label-MS Homo sapiens
122 BABAM1 29086
Affinity Capture-MS Homo sapiens
123 GFM1 85476
Proximity Label-MS Homo sapiens
124 AARS2  
Proximity Label-MS Homo sapiens
125 ARHGAP36  
Affinity Capture-MS Homo sapiens
126 TEFM  
Proximity Label-MS Homo sapiens
127 CIT 11113
Affinity Capture-MS Homo sapiens
128 NDEL1 81565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 BRD1 23774
Affinity Capture-MS Homo sapiens
130 C1QBP 708
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
131 LDLR 3949
Negative Genetic Homo sapiens
132 QRSL1  
Affinity Capture-MS Homo sapiens
133 C9orf72  
Affinity Capture-MS Homo sapiens
134 METTL17  
Proximity Label-MS Homo sapiens
135 PTCH1  
Affinity Capture-MS Homo sapiens
136 PRMT1 3276
Affinity Capture-MS Homo sapiens
137 TRIM43  
Affinity Capture-MS Homo sapiens
138 EP300 2033
Affinity Capture-MS Homo sapiens
139 NHLH1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLPX is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Mitochondrial protein degradation TAS Reactome





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