Gene description for MCM3
Gene name minichromosome maintenance complex component 3
Gene symbol MCM3
Other names/aliases HCC5
P1-MCM3
P1.h
RLFB
Species Homo sapiens
 Database cross references - MCM3
ExoCarta ExoCarta_4172
Entrez Gene 4172
HGNC 6945
MIM 602693
UniProt P25205  
 MCM3 identified in exosomes derived from the following tissue/cell type
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MCM3
Molecular Function
    ATP binding GO:0005524 IEA
    DNA helicase activity GO:0003678 IEA
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
Biological Process
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    mitotic cell cycle GO:0000278 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    DNA replication GO:0006260 TAS
    DNA replication initiation GO:0006270 IEA
    DNA duplex unwinding GO:0032508 IEA
Subcellular Localization
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    MCM complex GO:0042555 IDA
    alpha DNA polymerase:primase complex GO:0005658 TAS
    nucleus GO:0005634 IDA
    centrosome GO:0005813 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified MCM3 in exosomes
1
Experiment ID 219
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 220
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 221
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASK  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 MCM7 4176
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
3 WDR8  
Affinity Capture-MS Homo sapiens
4 CDC45L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 MCM5 4174
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 RPA2 6118
Two-hybrid Homo sapiens
7 SNCG 6623
Affinity Capture-MS Homo sapiens
8 PAK2 5062
Affinity Capture-MS Homo sapiens
9 CDC6  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
10 ORC5L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 PNKP 11284
Affinity Capture-MS Homo sapiens
12 MCM10 55388
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 CDK2 1017
Affinity Capture-MS Homo sapiens
14 ENSA  
Affinity Capture-MS Homo sapiens
15 PTP4A3  
Affinity Capture-MS Homo sapiens
16 STAT1 6772
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
17 MCM2 4171
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 CCDC5  
Affinity Capture-MS Homo sapiens
19 ORC4L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
20 CDC7  
Biochemical Activity Homo sapiens
21 MCM6 4175
Affinity Capture-MS Homo sapiens
22 MCM3AP  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
23 CDC5L 988
Affinity Capture-MS Homo sapiens
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