Gene description for LYN
Gene name LYN proto-oncogene, Src family tyrosine kinase
Gene symbol LYN
Other names/aliases JTK8
p53Lyn
p56Lyn
Species Homo sapiens
 Database cross references - LYN
ExoCarta ExoCarta_4067
Entrez Gene 4067
HGNC 6735
MIM 165120
UniProt P07948  
 LYN identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for LYN
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IEA
    ion channel binding GO:0044325 IPI
    glycosphingolipid binding GO:0043208 IEA
    receptor binding GO:0005102 IBA
    platelet-derived growth factor receptor binding GO:0005161 IEA
    integrin binding GO:0005178 IEA
    SH3 domain binding GO:0017124 IEA
    receptor signaling protein tyrosine kinase activity GO:0004716 TAS
    protein binding GO:0005515 IPI
    protein tyrosine kinase activity GO:0004713 TAS
    phosphoprotein binding GO:0051219 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 TAS
    enzyme binding GO:0019899 IPI
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of cell proliferation GO:0008284 ISS
    negative regulation of B cell proliferation GO:0030889 IBA
    dendritic cell differentiation GO:0097028 ISS
    cellular response to DNA damage stimulus GO:0006974 IDA
    regulation of cytokine production GO:0001817 ISS
    regulation of platelet aggregation GO:0090330 ISS
    positive regulation of cellular component movement GO:0051272 IDA
    signal transduction GO:0007165 TAS
    histamine secretion by mast cell GO:0002553 IEA
    regulation of cytokine secretion GO:0050707 IEA
    positive regulation of oligodendrocyte progenitor proliferation GO:0070447 IEA
    regulation of B cell apoptotic process GO:0002902 IBA
    cytokine secretion GO:0050663 IEA
    negative regulation of MAP kinase activity GO:0043407 ISS
    regulation of mast cell activation GO:0033003 ISS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    cellular response to peptide hormone stimulus GO:0071375 IBA
    positive regulation of glial cell proliferation GO:0060252 IEA
    T cell costimulation GO:0031295 TAS
    positive regulation of mast cell proliferation GO:0070668 IMP
    negative regulation of intracellular signal transduction GO:1902532 ISS
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    blood coagulation GO:0007596 TAS
    B cell receptor signaling pathway GO:0050853 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    negative regulation of cell proliferation GO:0008285 IMP
    positive regulation of dendritic cell apoptotic process GO:2000670 ISS
    erythrocyte differentiation GO:0030218 ISS
    positive regulation of neuron projection development GO:0010976 IMP
    signal transduction by protein phosphorylation GO:0023014 TAS
    Fc receptor mediated inhibitory signaling pathway GO:0002774 ISS
    cellular response to extracellular stimulus GO:0031668 IEA
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
    positive regulation of Fc receptor mediated stimulatory signaling pathway GO:0060369 IEA
    leukocyte migration GO:0050900 TAS
    negative regulation of myeloid leukocyte differentiation GO:0002762 IEA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 ISS
    regulation of erythrocyte differentiation GO:0045646 ISS
    response to sterol depletion GO:0006991 IEA
    negative regulation of toll-like receptor 2 signaling pathway GO:0034136 ISS
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    oligodendrocyte development GO:0014003 IEA
    response to organic cyclic compound GO:0014070 IEA
    immune response-regulating cell surface receptor signaling pathway GO:0002768 TAS
    response to hormone GO:0009725 ISS
    lipopolysaccharide-mediated signaling pathway GO:0031663 ISS
    neuron projection development GO:0031175 IBA
    response to carbohydrate GO:0009743 IEA
    positive regulation of stress-activated protein kinase signaling cascade GO:0070304 IDA
    negative regulation of protein phosphorylation GO:0001933 ISS
    positive regulation of B cell receptor signaling pathway GO:0050861 IEA
    negative regulation of mast cell proliferation GO:0070667 ISS
    viral process GO:0016032 IEA
    protein autophosphorylation GO:0046777 IDA
    response to axon injury GO:0048678 IEA
    response to amino acid GO:0043200 IEA
    platelet degranulation GO:0002576 ISS
    regulation of B cell receptor signaling pathway GO:0050855 ISS
    regulation of cell adhesion mediated by integrin GO:0033628 IMP
    response to insulin GO:0032868 IEA
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    B cell homeostasis GO:0001782 ISS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of cell migration GO:0030335 IEA
    innate immune response GO:0045087 TAS
    negative regulation of immune response GO:0050777 TAS
    cellular response to heat GO:0034605 IEA
    regulation of mast cell degranulation GO:0043304 ISS
    tolerance induction to self antigen GO:0002513 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IMP
    axon guidance GO:0007411 TAS
    platelet activation GO:0030168 TAS
    protein phosphorylation GO:0006468 IDA
    Fc receptor mediated stimulatory signaling pathway GO:0002431 ISS
    regulation of monocyte chemotaxis GO:0090025 IMP
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    cellular response to retinoic acid GO:0071300 IMP
    negative regulation of toll-like receptor 4 signaling pathway GO:0034144 ISS
    response to toxic substance GO:0009636 IEA
    response to drug GO:0042493 IEA
    regulation of inflammatory response GO:0050727 IEA
    regulation of protein phosphorylation GO:0001932 TAS
Subcellular Localization
    mitochondrial crista GO:0030061 IEA
    cytosol GO:0005829 TAS
    mast cell granule GO:0042629 IEA
    mitochondrial intermembrane space GO:0005758 IEA
    nucleus GO:0005634 ISS
    postsynaptic density GO:0014069 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    Golgi apparatus GO:0005794 IDA
    extracellular exosome GO:0070062 IDA
    integrin alpha2-beta1 complex GO:0034666 IEA
    membrane raft GO:0045121 IDA
    plasma membrane GO:0005886 TAS
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified LYN in exosomes
1
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 285
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 286
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
12
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
18
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
19
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LYN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BTK 695
Reconstituted Complex Homo sapiens
2 GRIA3  
Invivo Homo sapiens
3 NMT1 4836
Biochemical Activity Homo sapiens
4 PRAM-1  
Affinity Capture-Western Homo sapiens
5 CSNK2B 1460
Two-hybrid Homo sapiens
6 GAB3  
Reconstituted Complex Homo sapiens
7 BANK1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 CBL 867
Affinity Capture-Western Homo sapiens
9 CD22 933
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
10 INPP5D 3635
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 EVL 51466
Reconstituted Complex Homo sapiens
12 CD72  
Biochemical Activity Homo sapiens
13 MME 4311
Affinity Capture-Western Homo sapiens
14 NEDD9 4739
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 BCAR1 9564
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
16 FCER1G 2207
Invivo Homo sapiens
17 KIT 3815
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
18 MAPK3 5595
Affinity Capture-Western Homo sapiens
19 TRAT1 50852
Reconstituted Complex Homo sapiens
20 RPS6KB1  
Biochemical Activity Homo sapiens
21 PPP1R8 5511
Invitro Homo sapiens
22 PDE4A  
Reconstituted Complex Homo sapiens
23 UNC119  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 SKAP1 8631
Reconstituted Complex Homo sapiens
25 GAB2 9846
Affinity Capture-Western Homo sapiens
26 PRKDC 5591
Reconstituted Complex Homo sapiens
27 CDK1 983
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
28 PAG1  
Reconstituted Complex Homo sapiens
29 TYK2 7297
Reconstituted Complex Homo sapiens
30 SKAP2 8935
Affinity Capture-Western Homo sapiens
31 CD36 948
Affinity Capture-Western Homo sapiens
32 FCGR2A 2212
Affinity Capture-Western Homo sapiens
33 TEC 7006
Affinity Capture-Western Homo sapiens
34 EPOR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 PTPN6 5777
Reconstituted Complex Homo sapiens
36 CDK2 1017
Invivo Homo sapiens
Invitro Homo sapiens
37 DOK1 1796
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
38 CSF2RB  
Invivo Homo sapiens
Protein-peptide Homo sapiens
39 RPS6KB2  
Biochemical Activity Homo sapiens
40 IL7R  
Invivo Homo sapiens
41 PLCG2 5336
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 CSF3R  
Invitro Homo sapiens
43 HCLS1  
Invivo Homo sapiens
Invitro Homo sapiens
44 CTLA4  
Invivo Homo sapiens
Invitro Homo sapiens
45 LCP2 3937
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 PTK2B 2185
Affinity Capture-Western Homo sapiens
47 FCAR  
Invivo Homo sapiens
48 CHST15 51363
Invivo Homo sapiens
49 MUC1 4582
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 SRC 6714
Protein-peptide Homo sapiens
51 PPP1R15A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 PIK3CG  
Invivo Homo sapiens
53 SYK 6850
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
54 TRPV4 59341
Invivo Homo sapiens
Invitro Homo sapiens
55 GP6 51206
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
56 JAK2 3717
Reconstituted Complex Homo sapiens
57 CSF1R  
Invivo Homo sapiens
58 PECAM1 5175
Affinity Capture-Western Homo sapiens
59 CBLC  
Invitro Homo sapiens
60 SHC1 6464
Invitro Homo sapiens
61 CD24  
Invivo Homo sapiens
62 CDK4 1019
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here