Gene description for ADCY9
Gene name adenylate cyclase 9
Gene symbol ADCY9
Other names/aliases AC9
Species Homo sapiens
 Database cross references - ADCY9
ExoCarta ExoCarta_115
Vesiclepedia VP_115
Entrez Gene 115
HGNC 240
MIM 603302
UniProt O60503  
 ADCY9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ADCY9
Molecular Function
    adenylate cyclase activity GO:0004016 IBA
    adenylate cyclase activity GO:0004016 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    in utero embryonic development GO:0001701 IEA
    cAMP biosynthetic process GO:0006171 IDA
    signal transduction GO:0007165 TAS
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    intracellular signal transduction GO:0035556 IEA
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
 Experiment description of studies that identified ADCY9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADCY9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 MBLAC2 153364
Affinity Capture-MS Homo sapiens
5 TMEM143  
Affinity Capture-MS Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 ITPRIPL1  
Affinity Capture-MS Homo sapiens
10 GJD3  
Proximity Label-MS Homo sapiens
11 LAMP2 3920
Proximity Label-MS Homo sapiens
12 DNAJC25 548645
Proximity Label-MS Homo sapiens
13 PTPN1 5770
Proximity Label-MS Homo sapiens
14 STX4 6810
Proximity Label-MS Homo sapiens
15 CTXN1  
Affinity Capture-MS Homo sapiens
16 MS4A15  
Affinity Capture-MS Homo sapiens
17 B3GAT1  
Proximity Label-MS Homo sapiens
18 SLC20A1 6574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MLLT4 4301
Proximity Label-MS Homo sapiens
20 RPRM  
Affinity Capture-MS Homo sapiens
21 COX7A2L 9167
Affinity Capture-MS Homo sapiens
22 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SSR1 6745
Proximity Label-MS Homo sapiens
25 C3orf52  
Affinity Capture-MS Homo sapiens
26 RPN2 6185
Proximity Label-MS Homo sapiens
27 NLGN2 57555
Affinity Capture-MS Homo sapiens
28 STX6 10228
Proximity Label-MS Homo sapiens
29 NOS1  
Affinity Capture-MS Homo sapiens
30 DNAJC7 7266
Proximity Label-MS Homo sapiens
31 RAB35 11021
Proximity Label-MS Homo sapiens
32 CLGN 1047
Affinity Capture-MS Homo sapiens
33 ALG5 29880
Affinity Capture-MS Homo sapiens
34 LAMTOR1 55004
Proximity Label-MS Homo sapiens
35 LMAN1 3998
Proximity Label-MS Homo sapiens
36 NUP155 9631
Proximity Label-MS Homo sapiens
37 PXMP2  
Proximity Label-MS Homo sapiens
38 RAB5A 5868
Proximity Label-MS Homo sapiens
39 EXTL3 2137
Affinity Capture-MS Homo sapiens
40 ANKMY2 57037
Affinity Capture-MS Homo sapiens
41 JMJD6 23210
Affinity Capture-MS Homo sapiens
42 FUT8 2530
Affinity Capture-MS Homo sapiens
43 EMD 2010
Proximity Label-MS Homo sapiens
44 CXADR 1525
Proximity Label-MS Homo sapiens
45 AVPR1B  
Affinity Capture-MS Homo sapiens
46 LYN 4067
Proximity Label-MS Homo sapiens
47 MCAM 4162
Proximity Label-MS Homo sapiens
48 RAB4A 5867
Proximity Label-MS Homo sapiens
49 DNAJC5 80331
Proximity Label-MS Homo sapiens
50 CDH1 999
Proximity Label-MS Homo sapiens
51 DIRAS3  
Proximity Label-MS Homo sapiens
52 DNAJC16  
Proximity Label-MS Homo sapiens
53 RPN1 6184
Proximity Label-MS Homo sapiens
54 ELOVL5 60481
Proximity Label-MS Homo sapiens
55 SEC61B 10952
Proximity Label-MS Homo sapiens
56 REEP5 7905
Proximity Label-MS Homo sapiens
57 SEC63 11231
Proximity Label-MS Homo sapiens
58 TTYH1  
Affinity Capture-MS Homo sapiens
59 LRRC59 55379
Proximity Label-MS Homo sapiens
60 PANX1 24145
Proximity Label-MS Homo sapiens
61 ZDHHC12  
Affinity Capture-MS Homo sapiens
62 LAMP1 3916
Proximity Label-MS Homo sapiens
63 BCAP31 10134
Proximity Label-MS Homo sapiens
64 HSD3B7 80270
Proximity Label-MS Homo sapiens
65 STIM1 6786
Proximity Label-MS Homo sapiens
66 LGALS9B 284194
Affinity Capture-MS Homo sapiens
67 FKBP8 23770
Proximity Label-MS Homo sapiens
68 ARF6 382
Proximity Label-MS Homo sapiens
69 OMA1  
Affinity Capture-MS Homo sapiens
70 TMEM194A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 CD27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 DNAJC18  
Affinity Capture-MS Homo sapiens
73 NLGN1  
Affinity Capture-MS Homo sapiens
74 ATP2A1 487
Proximity Label-MS Homo sapiens
75 ZMYND8 23613
Affinity Capture-MS Homo sapiens
76 RAB9A 9367
Proximity Label-MS Homo sapiens
77 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 CISD2 493856
Affinity Capture-MS Homo sapiens
79 TGFA  
Affinity Capture-MS Homo sapiens
80 FNDC3A 22862
Affinity Capture-MS Homo sapiens
81 DHFRL1  
Proximity Label-MS Homo sapiens
82 RHOB 388
Proximity Label-MS Homo sapiens
83 CYP2C9  
Proximity Label-MS Homo sapiens
84 HAX1  
Proximity Label-MS Homo sapiens
85 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 LEMD3  
Affinity Capture-MS Homo sapiens
87 MBOAT7 79143
Affinity Capture-MS Homo sapiens
88 HMGCR  
Affinity Capture-MS Homo sapiens
89 C1orf43 25912
Affinity Capture-MS Homo sapiens
90 SIDT2 51092
Affinity Capture-MS Homo sapiens
91 TPTE  
Proximity Label-MS Homo sapiens
92 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 CYP51A1 1595
Affinity Capture-MS Homo sapiens
94 HSPB1 3315
Two-hybrid Homo sapiens
95 ERGIC1 57222
Proximity Label-MS Homo sapiens
96 C11orf52 91894
Proximity Label-MS Homo sapiens
97 ANAPC2 29882
Proximity Label-MS Homo sapiens
98 CLMN  
Affinity Capture-MS Homo sapiens
99 RAB2A 5862
Proximity Label-MS Homo sapiens
100 SEC62 7095
Proximity Label-MS Homo sapiens
101 DNAJC5B  
Proximity Label-MS Homo sapiens
102 RAB11A 8766
Proximity Label-MS Homo sapiens
103 PXYLP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 EPHA2 1969
Proximity Label-MS Homo sapiens
105 AREG 374
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 IER3IP1 51124
Affinity Capture-MS Homo sapiens
107 DNAJC1 64215
Proximity Label-MS Homo sapiens
108 KRAS 3845
Proximity Label-MS Homo sapiens
109 RAB5C 5878
Proximity Label-MS Homo sapiens
110 PIANP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 CKAP4 10970
Proximity Label-MS Homo sapiens
112 CAV1 857
Proximity Label-MS Homo sapiens
113 POPDC2  
Affinity Capture-MS Homo sapiens
114 LRRTM3  
Affinity Capture-MS Homo sapiens
115 DERL1 79139
Proximity Label-MS Homo sapiens
116 DNAJC22  
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ADCY9 is involved
PathwayEvidenceSource
Activation of GABAB receptors IEA Reactome
Activation of GABAB receptors TAS Reactome
Adenylate cyclase activating pathway TAS Reactome
Adenylate cyclase inhibitory pathway IEA Reactome
Adenylate cyclase inhibitory pathway TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production IEA Reactome
ADORA2B mediated anti-inflammatory cytokines production TAS Reactome
Anti-inflammatory response favouring Leishmania parasite infection IEA Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Aquaporin-mediated transport TAS Reactome
Ca-dependent events TAS Reactome
Calmodulin induced events TAS Reactome
CaM pathway TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
DAG and IP3 signaling TAS Reactome
Disease IEA Reactome
Disease TAS Reactome
FCGR3A-mediated IL10 synthesis TAS Reactome
G alpha (i) signalling events IEA Reactome
G alpha (i) signalling events TAS Reactome
G alpha (s) signalling events IEA Reactome
G alpha (s) signalling events TAS Reactome
G alpha (z) signalling events IEA Reactome
G alpha (z) signalling events TAS Reactome
G-protein mediated events IEA Reactome
G-protein mediated events TAS Reactome
GABA B receptor activation IEA Reactome
GABA B receptor activation TAS Reactome
GABA receptor activation IEA Reactome
GABA receptor activation TAS Reactome
Glucagon signaling in metabolic regulation TAS Reactome
GPCR downstream signalling IEA Reactome
GPCR downstream signalling TAS Reactome
GPER1 signaling IEA Reactome
GPER1 signaling TAS Reactome
Hedgehog 'off' state TAS Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Integration of energy metabolism TAS Reactome
Intracellular signaling by second messengers TAS Reactome
Leishmania infection IEA Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival IEA Reactome
Leishmania parasite growth and survival TAS Reactome
Metabolism TAS Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling IEA Reactome
Opioid Signalling TAS Reactome
Parasitic Infection Pathways IEA Reactome
Parasitic Infection Pathways TAS Reactome
PKA activation TAS Reactome
PKA activation in glucagon signalling TAS Reactome
PKA-mediated phosphorylation of CREB TAS Reactome
PLC beta mediated events TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Hedgehog TAS Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome





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