Gene description for NOTCH2
Gene name notch 2
Gene symbol NOTCH2
Other names/aliases AGS2
HJCYS
hN2
Species Homo sapiens
 Database cross references - NOTCH2
ExoCarta ExoCarta_4853
Entrez Gene 4853
HGNC 7882
MIM 600275
UniProt Q04721  
 NOTCH2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NOTCH2
Molecular Function
    receptor activity GO:0004872 NAS
    ligand-activated RNA polymerase II transcription factor binding transcription factor activity GO:0038049 TAS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of Ras protein signal transduction GO:0046579 IDA
    inflammatory response to antigenic stimulus GO:0002437 IEA
    positive regulation of apoptotic process GO:0043065 IEA
    negative regulation of apoptotic process GO:0043066 TAS
    stem cell maintenance GO:0019827 TAS
    pulmonary valve morphogenesis GO:0003184 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    placenta blood vessel development GO:0060674 IEA
    multicellular organismal development GO:0007275 NAS
    ciliary body morphogenesis GO:0061073 IEA
    Notch signaling pathway GO:0007219 TAS
    interleukin-4 secretion GO:0072602 IEA
    determination of left/right symmetry GO:0007368 IEA
    nervous system development GO:0007399 NAS
    positive regulation of cell proliferation GO:0008284 IEA
    cell cycle arrest GO:0007050 IDA
    embryonic limb morphogenesis GO:0030326 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    myeloid dendritic cell differentiation GO:0043011 IEA
    cell growth GO:0016049 IDA
    defense response to bacterium GO:0042742 IEA
    apoptotic process GO:0006915 TAS
    bone remodeling GO:0046849 IMP
    negative regulation of cell proliferation GO:0008285 IDA
    Notch signaling involved in heart development GO:0061314 IC
    gene expression GO:0010467 TAS
    atrial septum morphogenesis GO:0060413 IMP
    intracellular receptor signaling pathway GO:0030522 TAS
    cell fate determination GO:0001709 TAS
    morphogenesis of an epithelial sheet GO:0002011 IEA
    Notch receptor processing GO:0007220 TAS
    hemopoiesis GO:0030097 TAS
    humoral immune response GO:0006959 IEA
    in utero embryonic development GO:0001701 IEA
    organ morphogenesis GO:0009887 IEP
    positive regulation of BMP signaling pathway GO:0030513 IEA
    regulation of transcription, DNA-templated GO:0006355 TAS
Subcellular Localization
    cell surface GO:0009986 IDA
    receptor complex GO:0043235 IDA
    membrane GO:0016020 IDA
    cilium GO:0005929 IEA
    nucleus GO:0005634 IDA
    integral component of plasma membrane GO:0005887 IDA
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 TAS
    nucleoplasm GO:0005654 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified NOTCH2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NOTCH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBPSUH  
Two-hybrid Homo sapiens
2 DLL1  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 JAG2  
Invivo Homo sapiens
4 MAML1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 JAG1 182
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
6 GSK3B 2932
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CNTN1 1272
Affinity Capture-Western Homo sapiens
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