Gene description for LMTK2
Gene name lemur tyrosine kinase 2
Gene symbol LMTK2
Other names/aliases AATYK2
BREK
KPI-2
KPI2
LMR2
PPP1R100
cprk
Species Homo sapiens
 Database cross references - LMTK2
ExoCarta ExoCarta_22853
Vesiclepedia VP_22853
Entrez Gene 22853
HGNC 17880
MIM 610989
UniProt Q8IWU2  
 LMTK2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LMTK2
Molecular Function
    protein kinase activity GO:0004672 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein phosphatase inhibitor activity GO:0004864 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 NAS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    myosin VI binding GO:0070853 IPI
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    receptor recycling GO:0001881 IMP
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IMP
    transferrin transport GO:0033572 IMP
    early endosome to late endosome transport GO:0045022 IMP
    protein autophosphorylation GO:0046777 IDA
Subcellular Localization
    early endosome GO:0005769 IDA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 IDA
    growth cone GO:0030426 IEA
    neuronal cell body GO:0043025 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 IDA
 Experiment description of studies that identified LMTK2 in exosomes
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LMTK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DUSP19  
Two-hybrid Homo sapiens
2 DENND6A 201627
Proximity Label-MS Homo sapiens
3 CDC14A  
Two-hybrid Homo sapiens
4 PPM1K  
Two-hybrid Homo sapiens
5 PTPRM 5797
Proximity Label-MS Homo sapiens
6 PPP1CB 5500
Two-hybrid Homo sapiens
7 PPP1R3A  
Affinity Capture-MS Homo sapiens
8 SIRT6  
Affinity Capture-MS Homo sapiens
9 OCLN 100506658
Proximity Label-MS Homo sapiens
10 ARRDC5  
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 PTPN6 5777
Two-hybrid Homo sapiens
13 GJA1 2697
Proximity Label-MS Homo sapiens
14 RAB9A 9367
Proximity Label-MS Homo sapiens
15 PPP1CA 5499
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
16 LCK 3932
Proximity Label-MS Homo sapiens
17 LMTK2 22853
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
18 ILKAP 80895
Two-hybrid Homo sapiens
19 SLC34A2 10568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CDK5 1020
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
21 LYN 4067
Proximity Label-MS Homo sapiens
22 DUSP6 1848
Two-hybrid Homo sapiens
23 RAB4A 5867
Proximity Label-MS Homo sapiens
24 PPP1CC 5501
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 DUSP14 11072
Two-hybrid Homo sapiens
26 CDC25C  
Two-hybrid Homo sapiens
27 Lyplal1  
Affinity Capture-MS Mus musculus
28 MYO6 4646
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
29 TPTE2  
Two-hybrid Homo sapiens
30 ATP9A 10079
Affinity Capture-MS Homo sapiens
31 PTPN12 5782
Two-hybrid Homo sapiens
32 SORT1 6272
Affinity Capture-MS Homo sapiens
33 RAB11A 8766
Proximity Label-MS Homo sapiens
34 PTPDC1  
Two-hybrid Homo sapiens
35 PPP3CB  
Two-hybrid Homo sapiens
36 PEX14 5195
Proximity Label-MS Homo sapiens
37 PPP1R2 5504
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
38 Coro1c 23790
Affinity Capture-MS Mus musculus
39 STYX  
Two-hybrid Homo sapiens
40 PTPRR  
Two-hybrid Homo sapiens
41 CAV1 857
Proximity Label-MS Homo sapiens
42 DUPD1  
Two-hybrid Homo sapiens
43 FLOT1 10211
Proximity Label-MS Homo sapiens
44 PPM1A 5494
Two-hybrid Homo sapiens
45 STX6 10228
Proximity Label-MS Homo sapiens
46 MTMR6 9107
Two-hybrid Homo sapiens
47 PPM1F 9647
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which LMTK2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here