Gene description for RASSF8
Gene name Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Gene symbol RASSF8
Other names/aliases C12orf2
HOJ1
Species Homo sapiens
 Database cross references - RASSF8
ExoCarta ExoCarta_11228
Vesiclepedia VP_11228
Entrez Gene 11228
HGNC 13232
MIM 608231
UniProt Q8NHQ8  
 RASSF8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RASSF8
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 IEA
 Experiment description of studies that identified RASSF8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RASSF8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 Wdr48  
Affinity Capture-MS Mus musculus
4 SFRP4 6424
Affinity Capture-MS Homo sapiens
5 LYN 4067
Proximity Label-MS Homo sapiens
6 CTNNA1 1495
Proximity Label-MS Homo sapiens
7 CEP44  
Affinity Capture-MS Homo sapiens
8 TP53BP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 ZBTB2 57621
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 PPP1CB 5500
Affinity Capture-MS Homo sapiens
11 CCDC136  
Two-hybrid Homo sapiens
12 OCLN 100506658
Proximity Label-MS Homo sapiens
13 POLR2H 5437
Affinity Capture-MS Homo sapiens
14 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 YWHAB 7529
Affinity Capture-MS Homo sapiens
16 PAK3  
Affinity Capture-MS Homo sapiens
17 GOLGA2 2801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PHOSPHO1  
Two-hybrid Homo sapiens
19 PARD3 56288
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 EPB41L4A 64097
Proximity Label-MS Homo sapiens
21 CTNNB1 1499
Proximity Label-MS Homo sapiens
22 PPP1CA 5499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PARD6A  
Affinity Capture-MS Homo sapiens
24 Pard6b  
Affinity Capture-MS Mus musculus
25 SETD2  
Affinity Capture-MS Homo sapiens
26 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TRIB1 10221
Affinity Capture-MS Homo sapiens
28 RAB11FIP1 80223
Affinity Capture-MS Homo sapiens
29 PRPH 5630
Proximity Label-MS Homo sapiens
30 PSMD4 5710
Two-hybrid Homo sapiens
31 KLC1 3831
Affinity Capture-Western Homo sapiens
32 CXADR 1525
Proximity Label-MS Homo sapiens
33 ARID1B 57492
Affinity Capture-MS Homo sapiens
34 ANKRD28 23243
Proximity Label-MS Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 LATS1  
Proximity Label-MS Homo sapiens
37 AGO4  
Affinity Capture-MS Homo sapiens
38 Arrb2  
Affinity Capture-MS Mus musculus
39 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 POC1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MAPRE3  
Proximity Label-MS Homo sapiens
42 NLN 57486
Affinity Capture-MS Homo sapiens
43 ZCCHC9  
Affinity Capture-MS Homo sapiens
44 EDA  
Affinity Capture-MS Homo sapiens
45 KDM1A 23028
Two-hybrid Homo sapiens
46 TEAD3  
Proximity Label-MS Homo sapiens
47 PPP1R13B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ZNF639  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MLLT4 4301
Proximity Label-MS Homo sapiens
50 YAP1 10413
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PDK1 5163
Affinity Capture-MS Homo sapiens
52 FLOT1 10211
Proximity Label-MS Homo sapiens
53 PXN 5829
Proximity Label-MS Homo sapiens
54 TBC1D24 57465
Affinity Capture-MS Homo sapiens
55 RAB3IP  
Affinity Capture-MS Homo sapiens
56 C17orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CORO1A 11151
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 BICD1 636
Proximity Label-MS Homo sapiens
59 AMOT 154796
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
60 ACTB 60
Proximity Label-MS Homo sapiens
61 PARP10 84875
Affinity Capture-MS Homo sapiens
62 RAB5C 5878
Affinity Capture-MS Homo sapiens
63 RHOB 388
Proximity Label-MS Homo sapiens
64 APPBP2  
Affinity Capture-MS Homo sapiens
65 PCM1 5108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 Yap1  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
68 CLEC16A  
Affinity Capture-MS Homo sapiens
69 PARD6B 84612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 MPDZ  
Affinity Capture-MS Homo sapiens
71 PRKCI 5584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TIMM13 26517
Affinity Capture-MS Homo sapiens
73 FRMD6 122786
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
74 TCOF1 6949
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RASSF8 is involved
No pathways found





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