Gene description for COASY
Gene name CoA synthase
Gene symbol COASY
Other names/aliases DPCK
NBIA6
NBP
PPAT
UKR1
pOV-2
Species Homo sapiens
 Database cross references - COASY
ExoCarta ExoCarta_80347
Vesiclepedia VP_80347
Entrez Gene 80347
HGNC 29932
MIM 609855
UniProt Q13057  
 COASY identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for COASY
Molecular Function
    dephospho-CoA kinase activity GO:0004140 IBA
    dephospho-CoA kinase activity GO:0004140 IDA
    dephospho-CoA kinase activity GO:0004140 TAS
    pantetheine-phosphate adenylyltransferase activity GO:0004595 IBA
    pantetheine-phosphate adenylyltransferase activity GO:0004595 IDA
    pantetheine-phosphate adenylyltransferase activity GO:0004595 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    purine ribonucleotide biosynthetic process GO:0009152 IEA
    coenzyme A biosynthetic process GO:0015937 IBA
    coenzyme A biosynthetic process GO:0015937 IDA
    coenzyme A biosynthetic process GO:0015937 IEA
    coenzyme A biosynthetic process GO:0015937 TAS
    ribonucleoside bisphosphate biosynthetic process GO:0034030 IEA
    purine nucleoside bisphosphate biosynthetic process GO:0034033 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial outer membrane GO:0005741 TAS
    mitochondrial matrix GO:0005759 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified COASY in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for COASY
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
2 CISD3 284106
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 LRRC15 131578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
6 PTPN11 5781
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
8 NPAS1  
Affinity Capture-MS Homo sapiens
9 PRRC2A 7916
Cross-Linking-MS (XL-MS) Homo sapiens
10 PRKY  
Affinity Capture-MS Homo sapiens
11 DNAJC21  
Proximity Label-MS Homo sapiens
12 PSG11  
Affinity Capture-MS Homo sapiens
13 CIB2  
Affinity Capture-MS Homo sapiens
14 CALML3 810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HEPHL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ASNS 440
Co-fractionation Homo sapiens
17 KIAA1958  
Two-hybrid Homo sapiens
18 RPA2 6118
Proximity Label-MS Homo sapiens
19 KCTD14 65987
Affinity Capture-MS Homo sapiens
20 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SAP18 10284
Affinity Capture-MS Homo sapiens
22 RFXANK  
Affinity Capture-MS Homo sapiens
23 MIB1 57534
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CLASRP  
Affinity Capture-MS Homo sapiens
25 PLBD1 79887
Affinity Capture-MS Homo sapiens
26 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FAHD1 81889
Affinity Capture-MS Homo sapiens
28 PARK2  
Affinity Capture-MS Homo sapiens
29 DCTN3 11258
Affinity Capture-MS Homo sapiens
30 Mib1  
Affinity Capture-MS Mus musculus
31 AMACR 23600
Affinity Capture-MS Homo sapiens
32 PCYT2 5833
Co-fractionation Homo sapiens
33 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DDX58 23586
Affinity Capture-RNA Homo sapiens
35 PKP1 5317
Affinity Capture-MS Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
37 LYN 4067
Biochemical Activity Homo sapiens
38 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
39 SSX2  
Affinity Capture-MS Homo sapiens
40 DUSP14 11072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 EFHD2 79180
Co-fractionation Homo sapiens
42 ACTA1 58
Affinity Capture-MS Homo sapiens
43 PPL 5493
Co-fractionation Homo sapiens
44 S100A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 RPS6KB1 6198
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 TSTA3 7264
Co-fractionation Homo sapiens
47 CBFA2T2  
Affinity Capture-MS Homo sapiens
48 KRT20 54474
Two-hybrid Homo sapiens
49 DNAI2  
Affinity Capture-MS Homo sapiens
50 APOOL 139322
Affinity Capture-MS Homo sapiens
51 CNTROB  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
52 KIF23 9493
Affinity Capture-MS Homo sapiens
53 GLRX3 10539
Co-fractionation Homo sapiens
54 SCGN  
Affinity Capture-MS Homo sapiens
55 DSG4 147409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CENPM  
Affinity Capture-MS Homo sapiens
57 METTL21B  
Affinity Capture-MS Homo sapiens
58 LGI1  
Affinity Capture-MS Homo sapiens
59 RPL28 6158
Affinity Capture-MS Homo sapiens
60 PPME1 51400
Co-fractionation Homo sapiens
61 ENO2 2026
Co-fractionation Homo sapiens
62 SRC 6714
Biochemical Activity Homo sapiens
63 SYK 6850
Biochemical Activity Homo sapiens
64 YPEL3  
Affinity Capture-MS Homo sapiens
65 FSCN1 6624
Affinity Capture-MS Homo sapiens
66 GMPS 8833
Co-fractionation Homo sapiens
67 CCT8L2  
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 CLIC4 25932
Co-fractionation Homo sapiens
70 KRAS 3845
Negative Genetic Homo sapiens
71 GALNT9  
Affinity Capture-MS Homo sapiens
72 DNAJB11 51726
Proximity Label-MS Homo sapiens
73 BTK 695
Biochemical Activity Homo sapiens
74 UBXN6 80700
Affinity Capture-MS Homo sapiens
75 USP32 84669
Affinity Capture-MS Homo sapiens
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