Gene description for GP1BB
Gene name glycoprotein Ib (platelet), beta polypeptide
Gene symbol GP1BB
Other names/aliases BDPLT1
BS
CD42C
GPIBB
Species Homo sapiens
 Database cross references - GP1BB
ExoCarta ExoCarta_2812
Vesiclepedia VP_2812
Entrez Gene 2812
HGNC 4440
MIM 138720
UniProt P13224  
 GP1BB identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for GP1BB
Molecular Function
    transmembrane signaling receptor activity GO:0004888 NAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    cell surface receptor signaling pathway GO:0007166 NAS
    blood coagulation, intrinsic pathway GO:0007597 IPI
    blood coagulation, intrinsic pathway GO:0007597 NAS
    positive regulation of platelet activation GO:0010572 IDA
    positive regulation of platelet activation GO:0010572 NAS
    platelet activation GO:0030168 NAS
    megakaryocyte development GO:0035855 ISO
    megakaryocyte development GO:0035855 NAS
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    release of sequestered calcium ion into cytosol GO:0051209 NAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    glycoprotein Ib-IX-V complex GO:1990779 IPI
    glycoprotein Ib-IX-V complex GO:1990779 NAS
 Experiment description of studies that identified GP1BB in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
6
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for GP1BB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTGIR  
Affinity Capture-MS Homo sapiens
2 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GPM6A 2823
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LYN 4067
Affinity Capture-Western Homo sapiens
6 FAM189B  
Affinity Capture-MS Homo sapiens
7 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NKAIN1  
Affinity Capture-MS Homo sapiens
9 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TMEM9 252839
Affinity Capture-MS Homo sapiens
11 CHRNA4  
Affinity Capture-MS Homo sapiens
12 TSPAN15 23555
Affinity Capture-MS Homo sapiens
13 GABRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 SIDT2 51092
Affinity Capture-MS Homo sapiens
15 TRAF4 9618
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
17 LDLRAD1  
Affinity Capture-MS Homo sapiens
18 AMIGO1  
Affinity Capture-MS Homo sapiens
19 SMIM5  
Affinity Capture-MS Homo sapiens
20 LRRC55  
Affinity Capture-MS Homo sapiens
21 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SLC39A8 64116
Affinity Capture-MS Homo sapiens
24 TMEM30B 161291
Affinity Capture-MS Homo sapiens
25 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
27 TGFB1I1 7041
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 FSHR  
Affinity Capture-MS Homo sapiens
29 NCF1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
30 LPAR1 1902
Affinity Capture-MS Homo sapiens
31 TMEM169  
Affinity Capture-MS Homo sapiens
32 LRRC8B  
Affinity Capture-MS Homo sapiens
33 SCN4A  
Affinity Capture-MS Homo sapiens
34 CLEC12B  
Affinity Capture-MS Homo sapiens
35 PLS1 5357
Affinity Capture-MS Homo sapiens
36 SAAL1 113174
Affinity Capture-MS Homo sapiens
37 CHRNB2  
Affinity Capture-MS Homo sapiens
38 PLD3 23646
Affinity Capture-MS Homo sapiens
39 CHRNB4  
Affinity Capture-MS Homo sapiens
40 SLC22A2 6582
Affinity Capture-MS Homo sapiens
41 A4GNT  
Affinity Capture-MS Homo sapiens
42 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GPR21  
Affinity Capture-MS Homo sapiens
44 ARMT1 79624
Affinity Capture-MS Homo sapiens
45 TRDN 10345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PTK2B 2185
Affinity Capture-Western Homo sapiens
48 TMEM74  
Affinity Capture-MS Homo sapiens
49 MICAL1 64780
Affinity Capture-MS Homo sapiens
50 CD3E 916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 UPK1A 11045
Affinity Capture-MS Homo sapiens
52 RHOA 387
Affinity Capture-MS Homo sapiens
53 HAUS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ZDHHC12  
Affinity Capture-MS Homo sapiens
55 ZACN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CCDC47 57003
Affinity Capture-MS Homo sapiens
57 CLRN2  
Affinity Capture-MS Homo sapiens
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