Gene description for TSNAX
Gene name translin-associated factor X
Gene symbol TSNAX
Other names/aliases TRAX
Species Homo sapiens
 Database cross references - TSNAX
ExoCarta ExoCarta_7257
Vesiclepedia VP_7257
Entrez Gene 7257
HGNC 12380
MIM 602964
UniProt Q99598  
 TSNAX identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TSNAX
Molecular Function
    DNA binding GO:0003677 TAS
    single-stranded DNA binding GO:0003697 IEA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA endonuclease activity GO:0004521 IBA
    protein binding GO:0005515 IPI
    A2A adenosine receptor binding GO:0031687 IEA
    sequence-specific DNA binding GO:0043565 IEA
    protein-containing complex binding GO:0044877 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    spermatogenesis GO:0007283 IEA
    cell differentiation GO:0030154 IEA
    siRNA processing GO:0030422 IDA
    regulatory ncRNA-mediated post-transcriptional gene silencing GO:0035194 NAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    endoribonuclease complex GO:1902555 IPI
 Experiment description of studies that identified TSNAX in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TSNAX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AKR1B10 57016
Affinity Capture-MS Homo sapiens
2 MRPS27 23107
Affinity Capture-MS Homo sapiens
3 MCM3 4172
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RALYL 138046
Two-hybrid Homo sapiens
5 EXOSC7 23016
Affinity Capture-MS Homo sapiens
6 NUF2  
Affinity Capture-MS Homo sapiens
7 ORC4  
Affinity Capture-MS Homo sapiens
8 SPRED2 200734
Two-hybrid Homo sapiens
9 GADD45G  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
11 LDB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 SMG7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 C9orf78 51759
Affinity Capture-MS Homo sapiens
14 C4BPA 722
Affinity Capture-MS Homo sapiens
15 CREM  
Affinity Capture-MS Homo sapiens
16 PTPN1 5770
Co-fractionation Homo sapiens
17 PRPF40A 55660
Affinity Capture-MS Homo sapiens
18 SNX6 58533
Co-fractionation Homo sapiens
19 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
20 KIAA1598 57698
Co-fractionation Homo sapiens
21 KIAA1429 25962
Affinity Capture-MS Homo sapiens
22 CAPZB 832
Co-fractionation Homo sapiens
23 MRFAP1L1  
Two-hybrid Homo sapiens
24 AKAP17A  
Affinity Capture-MS Homo sapiens
25 PFKL 5211
Affinity Capture-MS Homo sapiens
26 PCNT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 DYNC1LI2 1783
Co-fractionation Homo sapiens
28 TCF12  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 SOGA1 140710
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
30 HIRIP3  
Co-fractionation Homo sapiens
31 KIF5B 3799
Co-fractionation Homo sapiens
32 IMPDH2 3615
Co-fractionation Homo sapiens
33 HEXB 3074
Affinity Capture-MS Homo sapiens
34 GGCT 79017
Affinity Capture-MS Homo sapiens
35 DYNLL1 8655
Affinity Capture-MS Homo sapiens
36 PDF  
Affinity Capture-MS Homo sapiens
37 U2AF1 7307
Affinity Capture-MS Homo sapiens
38 C1QTNF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 FBL 2091
Proximity Label-MS Homo sapiens
40 ACOX3 8310
Co-fractionation Homo sapiens
41 PFN2 5217
Co-fractionation Homo sapiens
42 TSN 7247
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
43 CARD9 64170
Two-hybrid Homo sapiens
44 MLEC 9761
Affinity Capture-MS Homo sapiens
45 KIF14 9928
Affinity Capture-MS Homo sapiens
46 GAR1 54433
Affinity Capture-MS Homo sapiens
47 FAS 355
Proximity Label-MS Homo sapiens
48 CC2D1B 200014
Affinity Capture-MS Homo sapiens
49 TOP3B 8940
Affinity Capture-MS Homo sapiens
50 RFC5 5985
Two-hybrid Homo sapiens
51 LACC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 RGPD3  
Affinity Capture-MS Homo sapiens
53 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
54 Tsn 22099
Reconstituted Complex Mus musculus
55 SMU1 55234
Affinity Capture-MS Homo sapiens
56 LYN 4067
Affinity Capture-MS Homo sapiens
57 TAF11  
Affinity Capture-MS Homo sapiens
58 TPD52 7163
Affinity Capture-MS Homo sapiens
59 RRAS2 22800
Affinity Capture-MS Homo sapiens
60 UBE2I 7329
Biochemical Activity Homo sapiens
61 BLOC1S5 63915
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 ACVR1B 91
Affinity Capture-MS Homo sapiens
63 PBXIP1 57326
Two-hybrid Homo sapiens
64 KLC3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
65 WDYHV1  
Two-hybrid Homo sapiens
66 TAF12  
Affinity Capture-MS Homo sapiens
67 SLC16A3 9123
Affinity Capture-MS Homo sapiens
68 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
69 EVPL 2125
Affinity Capture-MS Homo sapiens
70 PRPF18  
Two-hybrid Homo sapiens
71 FYN 2534
Affinity Capture-MS Homo sapiens
72 YAP1 10413
Co-fractionation Homo sapiens
73 CEBPA  
Protein-peptide Homo sapiens
74 METTL1 4234
Co-fractionation Homo sapiens
75 HMGB2 3148
Two-hybrid Homo sapiens
76 FSTL1 11167
Affinity Capture-MS Homo sapiens
77 FAM133B 257415
Affinity Capture-MS Homo sapiens
78 CEP128  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 GOLGA3 2802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 IPO5 3843
Co-fractionation Homo sapiens
81 TXNL4A 10907
Affinity Capture-MS Homo sapiens
82 EIF6 3692
Co-fractionation Homo sapiens
83 NSMCE2  
Biochemical Activity Homo sapiens
84 NGFRAP1  
Two-hybrid Homo sapiens
85 ATP5H 10476
Affinity Capture-MS Homo sapiens
86 NAA15 80155
Co-fractionation Homo sapiens
87 TCP11L1 55346
Two-hybrid Homo sapiens
88 CUL3 8452
Affinity Capture-MS Homo sapiens
89 SYCE2  
Two-hybrid Homo sapiens
90 TOP3A  
Two-hybrid Homo sapiens
91 RBFOX1 54715
Affinity Capture-MS Homo sapiens
92 BAP1 8314
Affinity Capture-MS Homo sapiens
93 SEC24A 10802
Co-fractionation Homo sapiens
94 STRAP 11171
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 CDK5RAP2 55755
Two-hybrid Homo sapiens
96 EFTUD2 9343
Affinity Capture-MS Homo sapiens
97 LZTS2 84445
Two-hybrid Homo sapiens
98 COG6 57511
Two-hybrid Homo sapiens
99 STAT1 6772
Co-fractionation Homo sapiens
100 RPA3 6119
Proximity Label-MS Homo sapiens
101 FXR2 9513
Affinity Capture-MS Homo sapiens
102 RGPD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 TRIM27  
Affinity Capture-MS Homo sapiens
104 C1D  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
105 MAK16  
Affinity Capture-MS Homo sapiens
106 KIAA0368 23392
Affinity Capture-MS Homo sapiens
107 CSDE1 7812
Co-fractionation Homo sapiens
108 MRPL38  
Affinity Capture-MS Homo sapiens
109 CETN2 1069
Affinity Capture-MS Homo sapiens
110 FAM103A1  
Affinity Capture-MS Homo sapiens
111 TCF25 22980
Affinity Capture-MS Homo sapiens
112 AIMP1 9255
Two-hybrid Homo sapiens
113 KIF24  
Affinity Capture-MS Homo sapiens
114 KIF2A 3796
Phenotypic Suppression Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 ARFIP1 27236
Co-fractionation Homo sapiens
116 RPS3 6188
Affinity Capture-MS Homo sapiens
117 VPS28 51160
Two-hybrid Homo sapiens
118 TP53 7157
Dosage Rescue Homo sapiens
119 RGPD8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 C9orf114  
Affinity Capture-MS Homo sapiens
121 MTRR  
Affinity Capture-MS Homo sapiens
122 FXR1 8087
Affinity Capture-MS Homo sapiens
123 BOP1 23246
Co-fractionation Homo sapiens
124 PDCD6 10016
Affinity Capture-MS Homo sapiens
125 SYT17  
Two-hybrid Homo sapiens
126 DMWD  
Affinity Capture-MS Homo sapiens
127 RNF20 56254
Affinity Capture-MS Homo sapiens
128 DDX58 23586
Affinity Capture-RNA Homo sapiens
129 EIF4B 1975
Co-fractionation Homo sapiens
130 PRDX1 5052
Affinity Capture-MS Homo sapiens
131 PRKAR1A 5573
Co-fractionation Homo sapiens
132 EMILIN1 11117
Two-hybrid Homo sapiens
133 LDOC1  
Two-hybrid Homo sapiens
134 NT5DC2 64943
Affinity Capture-MS Homo sapiens
135 CKAP4 10970
Two-hybrid Homo sapiens
136 KCNE4  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TSNAX is involved
PathwayEvidenceSource
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Small interfering RNA (siRNA) biogenesis TAS Reactome





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