Gene description for ADD2
Gene name adducin 2 (beta)
Gene symbol ADD2
Other names/aliases ADDB
Species Homo sapiens
 Database cross references - ADD2
ExoCarta ExoCarta_119
Entrez Gene 119
HGNC 244
MIM 102681
UniProt P35612  
 ADD2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ADD2
Molecular Function
    actin binding GO:0003779 TAS
    actin filament binding GO:0051015 IDA
    protein homodimerization activity GO:0042803 IPI
    structural molecule activity GO:0005198 IEA
    protein heterodimerization activity GO:0046982 IPI
    spectrin binding GO:0030507 IDA
    calmodulin binding GO:0005516 IEA
Biological Process
    actin cytoskeleton organization GO:0030036 IC
    transmembrane transport GO:0055085 TAS
    hemopoiesis GO:0030097 IEA
    positive regulation of protein binding GO:0032092 IDA
    barbed-end actin filament capping GO:0051016 IDA
    actin filament bundle assembly GO:0051017 IDA
    protein complex assembly GO:0006461 IDA
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    F-actin capping protein complex GO:0008290 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified ADD2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FYN 2534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 ADD1 118
Invivo Homo sapiens
3 SPTAN1 6709
Invitro Homo sapiens
4 RPH3A 22895
Invitro Homo sapiens
5 CALM1 801
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which ADD2 is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome





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