Gene description for SLC38A1
Gene name solute carrier family 38, member 1
Gene symbol SLC38A1
Other names/aliases ATA1
NAT2
SAT1
SNAT1
Species Homo sapiens
 Database cross references - SLC38A1
ExoCarta ExoCarta_81539
Vesiclepedia VP_81539
Entrez Gene 81539
HGNC 13447
MIM 608490
UniProt Q9H2H9  
 SLC38A1 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC38A1
Molecular Function
    amino acid:sodium symporter activity GO:0005283 ISS
    amino acid:sodium symporter activity GO:0005283 NAS
    neutral L-amino acid:sodium symporter activity GO:0005295 ISS
    proline:sodium symporter activity GO:0005298 ISS
    protein binding GO:0005515 IPI
    amino acid transmembrane transporter activity GO:0015171 TAS
    neutral L-amino acid transmembrane transporter activity GO:0015175 NAS
    L-amino acid transmembrane transporter activity GO:0015179 ISS
    L-amino acid transmembrane transporter activity GO:0015179 TAS
    L-glutamine transmembrane transporter activity GO:0015186 IBA
    L-glutamine transmembrane transporter activity GO:0015186 ISS
    glycine:sodium symporter activity GO:0015375 ISS
    alanine:sodium symporter activity GO:0015655 ISS
Biological Process
    neurotransmitter uptake GO:0001504 TAS
    amino acid transmembrane transport GO:0003333 IBA
    glutamine metabolic process GO:0006541 ISS
    amino acid transport GO:0006865 TAS
    glutamine transport GO:0006868 IBA
    glutamine transport GO:0006868 ISS
    female pregnancy GO:0007565 IEA
    gamma-aminobutyric acid biosynthetic process GO:0009449 ISS
    neutral amino acid transport GO:0015804 ISS
    neutral amino acid transport GO:0015804 NAS
    glycine transport GO:0015816 IEA
    regulation of synaptic transmission, GABAergic GO:0032228 ISS
    alanine transport GO:0032328 IEA
    sodium ion transmembrane transport GO:0035725 IEA
    amino acid import GO:0043090 ISS
    regulation of synaptic plasticity GO:0048167 ISS
    transport across blood-brain barrier GO:0150104 NAS
    positive regulation of L-glutamine import across plasma membrane GO:1901036 ISS
    L-alpha-amino acid transmembrane transport GO:1902475 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 NAS
    basolateral plasma membrane GO:0016323 ISS
    axon GO:0030424 IEA
    neuronal cell body GO:0043025 ISS
    extracellular exosome GO:0070062 HDA
    external side of apical plasma membrane GO:0098591 ISS
 Experiment description of studies that identified SLC38A1 in exosomes
1
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
10
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
11
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC38A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSMEM1  
Two-hybrid Homo sapiens
2 TMEM60  
Two-hybrid Homo sapiens
3 LOC100507537  
Two-hybrid Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 RAB35 11021
Proximity Label-MS Homo sapiens
6 AP2B1 163
Proximity Label-MS Homo sapiens
7 ANKFY1 51479
Proximity Label-MS Homo sapiens
8 EBAG9 9166
Proximity Label-MS Homo sapiens
9 PDCD1LG2 80380
Two-hybrid Homo sapiens
10 OCLN 100506658
Proximity Label-MS Homo sapiens
11 SERP2  
Two-hybrid Homo sapiens
12 CANX 821
Affinity Capture-MS Homo sapiens
13 ATP5J 522
Affinity Capture-MS Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
15 MARCKS 4082
Proximity Label-MS Homo sapiens
16 LAMP3  
Proximity Label-MS Homo sapiens
17 RAB5A 5868
Proximity Label-MS Homo sapiens
18 GJA1 2697
Proximity Label-MS Homo sapiens
19 SMIM3  
Two-hybrid Homo sapiens
20 AMIGO1  
Two-hybrid Homo sapiens
21 UNK  
Affinity Capture-RNA Homo sapiens
22 RAB11A 8766
Proximity Label-MS Homo sapiens
23 LAMP1 3916
Proximity Label-MS Homo sapiens
24 TPTE  
Proximity Label-MS Homo sapiens
25 GRM2 2912
Two-hybrid Homo sapiens
26 CD27  
Affinity Capture-MS Homo sapiens
27 NR3C1 2908
Proximity Label-MS Homo sapiens
28 ATG9A 79065
Proximity Label-MS Homo sapiens
29 LAMP2 3920
Proximity Label-MS Homo sapiens
30 SLC10A6  
Two-hybrid Homo sapiens
31 COX8A  
Proximity Label-MS Homo sapiens
32 IGFBP5 3488
Two-hybrid Homo sapiens
33 ARF6 382
Proximity Label-MS Homo sapiens
34 CD79A  
Two-hybrid Homo sapiens
35 SCN3B  
Two-hybrid Homo sapiens
36 ERGIC1 57222
Proximity Label-MS Homo sapiens
37 C11orf52 91894
Proximity Label-MS Homo sapiens
38 FFAR2  
Two-hybrid Homo sapiens
39 LMAN1 3998
Proximity Label-MS Homo sapiens
40 LYN 4067
Proximity Label-MS Homo sapiens
41 C1QL4  
Two-hybrid Homo sapiens
42 RAB4A 5867
Proximity Label-MS Homo sapiens
43 RAB3B 5865
Proximity Label-MS Homo sapiens
44 LAMTOR1 55004
Proximity Label-MS Homo sapiens
45 EPHA2 1969
Proximity Label-MS Homo sapiens
46 B3GAT1  
Proximity Label-MS Homo sapiens
47 GPR42  
Two-hybrid Homo sapiens
48 CDH1 999
Proximity Label-MS Homo sapiens
49 UBXN8  
Affinity Capture-MS Homo sapiens
50 MLLT4 4301
Proximity Label-MS Homo sapiens
51 VAMP5 10791
Affinity Capture-MS Homo sapiens
52 DIRAS3  
Proximity Label-MS Homo sapiens
53 RPRM  
Two-hybrid Homo sapiens
54 IL1RL1 9173
Two-hybrid Homo sapiens
55 SLC13A4  
Two-hybrid Homo sapiens
56 SLC10A1  
Two-hybrid Homo sapiens
57 BMP10  
Two-hybrid Homo sapiens
58 TMPPE  
Two-hybrid Homo sapiens
59 ARF1 375
Proximity Label-MS Homo sapiens
60 RAB2A 5862
Proximity Label-MS Homo sapiens
61 PMP22  
Two-hybrid Homo sapiens
62 CREB3  
Two-hybrid Homo sapiens
63 KRAS 3845
Proximity Label-MS Homo sapiens
64 RAB5C 5878
Proximity Label-MS Homo sapiens
65 RHOB 388
Proximity Label-MS Homo sapiens
66 ATP5C1 509
Affinity Capture-MS Homo sapiens
67 SCGB1D1  
Two-hybrid Homo sapiens
68 STX4 6810
Proximity Label-MS Homo sapiens
69 FATE1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 GPR152  
Two-hybrid Homo sapiens
71 TUSC5  
Two-hybrid Homo sapiens
72 CAV1 857
Proximity Label-MS Homo sapiens
73 ELOVL5 60481
Proximity Label-MS Homo sapiens
74 CTDSPL 10217
Proximity Label-MS Homo sapiens
75 PGRMC2 10424
Two-hybrid Homo sapiens
76 FLOT1 10211
Proximity Label-MS Homo sapiens
77 STX6 10228
Proximity Label-MS Homo sapiens
78 RAB9A 9367
Proximity Label-MS Homo sapiens
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