Gene description for DLG5
Gene name discs, large homolog 5 (Drosophila)
Gene symbol DLG5
Other names/aliases LP-DLG
P-DLG5
PDLG
Species Homo sapiens
 Database cross references - DLG5
ExoCarta ExoCarta_9231
Vesiclepedia VP_9231
Entrez Gene 9231
HGNC 2904
MIM 604090
UniProt Q8TDM6  
 DLG5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DLG5
Molecular Function
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IDA
    cytoskeletal protein binding GO:0008092 IDA
    signaling receptor complex adaptor activity GO:0030159 NAS
Biological Process
    epithelial to mesenchymal transition GO:0001837 IMP
    signal transduction GO:0007165 TAS
    negative regulation of cell population proliferation GO:0008285 TAS
    maintenance of cell polarity GO:0030011 IMP
    negative regulation of cell migration GO:0030336 IMP
    polarized epithelial cell differentiation GO:0030859 IEA
    midbrain development GO:0030901 IEA
    negative regulation of hippo signaling GO:0035331 IBA
    negative regulation of hippo signaling GO:0035331 IMP
    positive regulation of hippo signaling GO:0035332 IMP
    intracellular signal transduction GO:0035556 NAS
    negative regulation of T cell proliferation GO:0042130 IMP
    regulation of apoptotic process GO:0042981 IEA
    apical protein localization GO:0045176 IEA
    zonula adherens assembly GO:0045186 IEA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 NAS
    positive regulation of smoothened signaling pathway GO:0045880 ISS
    positive regulation of synapse assembly GO:0051965 ISS
    epithelial tube branching involved in lung morphogenesis GO:0060441 IEA
    neuroepithelial cell differentiation GO:0060563 IEA
    positive regulation of dendritic spine development GO:0060999 ISS
    protein-containing complex assembly GO:0065003 IEA
    protein localization to adherens junction GO:0071896 IEA
    metanephric collecting duct development GO:0072205 IEA
    cell-cell adhesion GO:0098609 NAS
    postsynapse organization GO:0099173 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IEA
    adherens junction GO:0005912 NAS
    postsynaptic density GO:0014069 ISS
    cell junction GO:0030054 IDA
    ciliary basal body GO:0036064 ISS
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified DLG5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DLG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
5 OCLN 100506658
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 PARD3 56288
Proximity Label-MS Homo sapiens
8 UBC 7316
Affinity Capture-MS Homo sapiens
9 STX4 6810
Proximity Label-MS Homo sapiens
10 SOX2  
Affinity Capture-MS Homo sapiens
11 MARK2 2011
Affinity Capture-MS Homo sapiens
12 RDX 5962
Proximity Label-MS Homo sapiens
13 MLLT4 4301
Proximity Label-MS Homo sapiens
14 DYRK1A 1859
Affinity Capture-MS Homo sapiens
15 KIF23 9493
Affinity Capture-MS Homo sapiens
16 ACTR3 10096
Proximity Label-MS Homo sapiens
17 IMPDH2 3615
Proximity Label-MS Homo sapiens
18 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 KRAS 3845
Proximity Label-MS Homo sapiens
Negative Genetic Homo sapiens
20 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 STX6 10228
Proximity Label-MS Homo sapiens
22 Naa11  
Affinity Capture-MS Mus musculus
23 POLR2C 5432
Proximity Label-MS Homo sapiens
24 MECP2 4204
Affinity Capture-MS Homo sapiens
25 RAB35 11021
Proximity Label-MS Homo sapiens
26 NCK1 4690
Affinity Capture-MS Homo sapiens
27 EBAG9 9166
Proximity Label-MS Homo sapiens
28 BTBD10 84280
Two-hybrid Homo sapiens
29 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PFN1 5216
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
31 BAG5 9529
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
32 KRT8 3856
Proximity Label-MS Homo sapiens
33 LYN 4067
Proximity Label-MS Homo sapiens
34 MAPRE1 22919
Proximity Label-MS Homo sapiens
35 DNAJB7  
Proximity Label-MS Homo sapiens
36 TERF1 7013
Two-hybrid Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 CDH1 999
Proximity Label-MS Homo sapiens
39 YWHAE 7531
Affinity Capture-MS Homo sapiens
40 DIRAS3  
Proximity Label-MS Homo sapiens
41 CD81 975
Two-hybrid Homo sapiens
42 YWHAQ 10971
Affinity Capture-MS Homo sapiens
43 DHFRL1  
Proximity Label-MS Homo sapiens
44 STIL  
Proximity Label-MS Homo sapiens
45 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 AGPS 8540
Proximity Label-MS Homo sapiens
47 DNAJB5  
Proximity Label-MS Homo sapiens
48 ACTG1 71
Affinity Capture-MS Homo sapiens
49 FGFR1OP  
Affinity Capture-MS Homo sapiens
50 YWHAH 7533
Affinity Capture-MS Homo sapiens
51 PHLPP1  
Proximity Label-MS Homo sapiens
52 GAN 8139
Affinity Capture-MS Homo sapiens
53 Myh9 17886
Affinity Capture-MS Mus musculus
54 CEP135  
Proximity Label-MS Homo sapiens
55 KRT18 3875
Proximity Label-MS Homo sapiens
56 EPB41L4A 64097
Proximity Label-MS Homo sapiens
57 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
58 CUL3 8452
Affinity Capture-MS Homo sapiens
59 YWHAZ 7534
Affinity Capture-MS Homo sapiens
60 CTTN 2017
Affinity Capture-MS Homo sapiens
61 HOOK3 84376
Proximity Label-MS Homo sapiens
62 SASS6 163786
Proximity Label-MS Homo sapiens
63 AURKB 9212
Affinity Capture-MS Homo sapiens
64 MAPRE3  
Proximity Label-MS Homo sapiens
65 TPM1 7168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 NEFM 4741
Proximity Label-MS Homo sapiens
67 SAV1  
Affinity Capture-MS Homo sapiens
68 DLST 1743
Two-hybrid Homo sapiens
69 GOLGA1  
Proximity Label-MS Homo sapiens
70 NDC80 10403
Proximity Label-MS Homo sapiens
71 SORBS3 10174
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
72 VEPH1 79674
Affinity Capture-MS Homo sapiens
73 AMOT 154796
Proximity Label-MS Homo sapiens
74 RHOB 388
Proximity Label-MS Homo sapiens
75 PCM1 5108
Proximity Label-MS Homo sapiens
76 CTNNA1 1495
Proximity Label-MS Homo sapiens
77 KRT19 3880
Proximity Label-MS Homo sapiens
78 FLOT1 10211
Proximity Label-MS Homo sapiens
79 PAFAH1B1 5048
Proximity Label-MS Homo sapiens
80 MARK3 4140
Affinity Capture-MS Homo sapiens
81 RNF43  
Proximity Label-MS Homo sapiens
82 ANKFY1 51479
Proximity Label-MS Homo sapiens
83 EZR 7430
Proximity Label-MS Homo sapiens
84 Cep135  
Affinity Capture-MS Mus musculus
85 LCK 3932
Proximity Label-MS Homo sapiens
86 SEPT10 151011
Proximity Label-MS Homo sapiens
87 DCTN2 10540
Proximity Label-MS Homo sapiens
88 C11orf52 91894
Proximity Label-MS Homo sapiens
89 CDK1 983
Affinity Capture-MS Homo sapiens
90 LATS1  
Proximity Label-MS Homo sapiens
91 ANAPC2 29882
Proximity Label-MS Homo sapiens
92 TRIM37  
Proximity Label-MS Homo sapiens
93 KDM1A 23028
Proximity Label-MS Homo sapiens
94 PXN 5829
Proximity Label-MS Homo sapiens
95 ANK3  
Proximity Label-MS Homo sapiens
96 CAV1 857
Proximity Label-MS Homo sapiens
97 LURAP1  
Affinity Capture-MS Homo sapiens
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