Gene description for PTPRC
Gene name protein tyrosine phosphatase, receptor type, C
Gene symbol PTPRC
Other names/aliases B220
CD45
CD45R
GP180
L-CA
LCA
LY5
T200
Species Homo sapiens
 Database cross references - PTPRC
ExoCarta ExoCarta_5788
Entrez Gene 5788
HGNC 9666
MIM 151460
UniProt P08575  
 PTPRC identified in exosomes derived from the following tissue/cell type
B cells 12519789    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for PTPRC
Molecular Function
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 TAS
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity GO:0004725 IDA
Biological Process
    positive regulation of antigen receptor-mediated signaling pathway GO:0050857 ISS
    protein dephosphorylation GO:0006470 ISS
    positive regulation of stem cell proliferation GO:2000648 IMP
    positive regulation of protein kinase activity GO:0045860 NAS
    positive regulation of hematopoietic stem cell migration GO:2000473 IMP
    T cell receptor signaling pathway GO:0050852 TAS
    regulation of cell cycle GO:0051726 ISS
    release of sequestered calcium ion into cytosol GO:0051209 ISS
    bone marrow development GO:0048539 IMP
    axon guidance GO:0007411 TAS
    negative regulation of T cell mediated cytotoxicity GO:0001915 ISS
    defense response to virus GO:0051607 ISS
    cell surface receptor signaling pathway GO:0007166 TAS
    negative regulation of cytokine-mediated signaling pathway GO:0001960 ISS
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    stem cell development GO:0048864 IMP
    cell cycle phase transition GO:0044770 IMP
    positive regulation of T cell proliferation GO:0042102 ISS
    positive regulation of B cell proliferation GO:0030890 IMP
    T cell differentiation GO:0030217 ISS
    dephosphorylation GO:0016311 ISS
    B cell receptor signaling pathway GO:0050853 ISS
    negative regulation of protein kinase activity GO:0006469 ISS
    negative regulation of cell adhesion involved in substrate-bound cell migration GO:0006933 IMP
    peptidyl-tyrosine dephosphorylation GO:0035335 TAS
    immunoglobulin biosynthetic process GO:0002378 IMP
    B cell proliferation GO:0042100 ISS
Subcellular Localization
    cell surface GO:0009986 IDA
    focal adhesion GO:0005925 ISS
    extracellular exosome GO:0070062 IDA
    integral component of plasma membrane GO:0005887 ISS
    external side of plasma membrane GO:0009897 IDA
    membrane raft GO:0045121 IEA
    membrane GO:0016020 IDA
    plasma membrane GO:0005886 TAS
    intracellular GO:0005622 ISS
 Experiment description of studies that identified PTPRC in exosomes
1
Experiment ID 11
ISEV standards
EV Biophysical techniques
HSC70|HSP90
EV Cytosolic markers
MHCII|CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTPRC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Invitro Homo sapiens
Invivo Homo sapiens
2 VAV1 7409
Invivo Homo sapiens
Invitro Homo sapiens
3 SEMA4D  
Invivo Homo sapiens
Invitro Homo sapiens
4 PRKCSH 5589
Affinity Capture-Western Homo sapiens
5 GRB2 2885
Invivo Homo sapiens
Invitro Homo sapiens
6 CD22 933
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
7 PAEP  
Co-localization Homo sapiens
8 CD4 920
FRET Homo sapiens
9 LCK 3932
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 LGALS1 3956
Invitro Homo sapiens
Invivo Homo sapiens
11 CD8A 925
FRET Homo sapiens
12 CD79A  
Invitro Homo sapiens
13 PPFIBP2 8495
Invitro Homo sapiens
14 CD247 919
Reconstituted Complex Homo sapiens
15 JAK3 3718
Invitro Homo sapiens
16 SKAP1 8631
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
17 FCGR3A  
Invivo Homo sapiens
18 PTPRCAP 5790
Reconstituted Complex Homo sapiens
19 LCP2 3937
Invivo Homo sapiens
20 ZAP70 7535
Invitro Homo sapiens
21 CD2 914
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
22 GANAB 23193
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 PPFIA1 8500
Invitro Homo sapiens
24 IRS1 3667
Invivo Homo sapiens
25 PLK3  
Affinity Capture-Western Homo sapiens
26 CD1D  
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PTPRC is involved
PathwayEvidenceSource
Other semaphorin interactions TAS Reactome
Phosphorylation of CD3 and TCR zeta chains TAS Reactome





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