Gene description for PAK4
Gene name p21 protein (Cdc42/Rac)-activated kinase 4
Gene symbol PAK4
Other names/aliases -
Species Homo sapiens
 Database cross references - PAK4
ExoCarta ExoCarta_10298
Entrez Gene 10298
HGNC 16059
MIM 605451
UniProt O96013  
 PAK4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PAK4
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    ATP binding GO:0005524 IEA
    Rac GTPase binding GO:0048365 IBA
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 NAS
Biological Process
    cell proliferation GO:0008283 TAS
    movement of cell or subcellular component GO:0006928 TAS
    cell growth GO:0016049 TAS
    cell migration GO:0016477 TAS
    apoptotic process GO:0006915 TAS
    activation of protein kinase activity GO:0032147 IBA
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    cytoskeleton organization GO:0007010 TAS
    cell cycle GO:0007049 IEA
    signal transduction by protein phosphorylation GO:0023014 IBA
    signal transduction GO:0007165 TAS
    regulation of apoptotic process GO:0042981 IBA
    regulation of MAPK cascade GO:0043408 IBA
    Rho protein signal transduction GO:0007266 IBA
    actin cytoskeleton organization GO:0030036 IBA
    regulation of mitotic cell cycle GO:0007346 IBA
Subcellular Localization
    focal adhesion GO:0005925 IDA
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 TAS
 Experiment description of studies that identified PAK4 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PAK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 CDC42 998
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 ITGAV 3685
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 RAC1 5879
Affinity Capture-Western Homo sapiens
5 YWHAG 7532
Affinity Capture-MS Homo sapiens
6 ITGB5 3693
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
7 LIMK1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
8 YWHAZ 7534
Affinity Capture-MS Homo sapiens
9 LRRK1  
Affinity Capture-MS Homo sapiens
10 YWHAQ 10971
Affinity Capture-MS Homo sapiens
11 DLST 1743
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PAK4 is involved
PathwayEvidenceSource
Activation of Rac TAS Reactome





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