Gene description for MUC1
Gene name mucin 1, cell surface associated
Gene symbol MUC1
Other names/aliases ADMCKD
ADMCKD1
CA 15-3
CD227
EMA
H23AG
KL-6
MAM6
MCD
MCKD
MCKD1
MUC-1
MUC-1/SEC
MUC-1/X
MUC1/ZD
PEM
PEMT
PUM
Species Homo sapiens
 Database cross references - MUC1
ExoCarta ExoCarta_4582
Vesiclepedia VP_4582
Entrez Gene 4582
HGNC 7508
MIM 158340
UniProt P15941  
 MUC1 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Breast cancer cells 19415654    
Breast milk 17641064    
Breast milk 17641064    
Colorectal cancer cells 36303008    
Colorectal cancer cells 36303008    
Epidermoid cancer cells 36303008    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 36303008    
Gastric cancer cells 36303008    
Glioma cells 36303008    
Lung cancer cells 36303008    
Lung cancer cells 36303008    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Tracheobronchial cells 19190083    
Urine 15326289    
Urine 19056867    
Urine 20224111    
Urine 20224111    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for MUC1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    p53 binding GO:0002039 IPI
    transcription coregulator activity GO:0003712 IDA
    protein binding GO:0005515 IPI
Biological Process
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 IDA
    negative regulation of transcription by competitive promoter binding GO:0010944 IDA
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IDA
    negative regulation of cell adhesion mediated by integrin GO:0033629 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 IEA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 IBA
    vesicle GO:0031982 HDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified MUC1 in sEVs
1
Experiment ID 76
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 65
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors "Staubach S, Razawi H, Hanisch FG."
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 46
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
4
Experiment ID 48
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
5
Experiment ID 1361
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
MORPH
ELISA
6
Experiment ID 1361
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
MORPH
ELISA
7
Experiment ID 1362
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
8
Experiment ID 1362
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
9
Experiment ID 1365
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Epidermoid cancer cells
Sample name A431
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
10
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 1363
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Gastric cancer cells
Sample name MKN45
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
14
Experiment ID 1363
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Gastric cancer cells
Sample name MKN45
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
15
Experiment ID 1364
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Gastric cancer cells
Sample name SNU484
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
16
Experiment ID 1364
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Gastric cancer cells
Sample name SNU484
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
17
Experiment ID 1367
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Glioma cells
Sample name GLI36
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
18
Experiment ID 1367
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Glioma cells
Sample name GLI36
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
19
Experiment ID 1366
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Lung cancer cells
Sample name H3255
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
20
Experiment ID 1366
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Lung cancer cells
Sample name H3255
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
21
Experiment ID 1368
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method MORPH
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Lung cancer cells
Sample name PC9
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
22
Experiment ID 1368
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 36303008    
Organism Homo sapiens
Experiment description A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples
Authors "Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H."
Journal name Nat Biomed Eng
Publication year 2023
Sample Lung cancer cells
Sample name PC9
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study MORPH
ELISA
23
Experiment ID 275
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
24
Experiment ID 274
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
25
Experiment ID 66
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
26
Experiment ID 34
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
Mass spectrometry
FACS
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK"
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
27
Experiment ID 13
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
28
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
29
Experiment ID 77
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
30
Experiment ID 78
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Bladder cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
31
Experiment ID 193
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 196
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
35
Experiment ID 197
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MUC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C2CD2L 9854
Two-hybrid Homo sapiens
2 SLC30A8  
Two-hybrid Homo sapiens
3 CSGALNACT2 55454
Two-hybrid Homo sapiens
4 HBD 3045
Affinity Capture-MS Homo sapiens
5 PRKCD 5580
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 WWP1 11059
Affinity Capture-MS Homo sapiens
7 CD53 963
Two-hybrid Homo sapiens
8 SMIM1 388588
Two-hybrid Homo sapiens
9 NUP62 23636
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 TMEM147 10430
Two-hybrid Homo sapiens
11 APC  
Affinity Capture-Western Homo sapiens
12 SLC35B4  
Two-hybrid Homo sapiens
13 MFSD5  
Two-hybrid Homo sapiens
14 TMEM59L  
Affinity Capture-MS Homo sapiens
15 PLN  
Two-hybrid Homo sapiens
16 APP 351
Two-hybrid Homo sapiens
17 MYADM 91663
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
18 TMEM128  
Two-hybrid Homo sapiens
19 GALNT4 8693
Biochemical Activity Homo sapiens
20 AQP2 359
Two-hybrid Homo sapiens
21 VKORC1 79001
Two-hybrid Homo sapiens
22 TMEM86A  
Two-hybrid Homo sapiens
23 SRC 6714
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 SELK  
Two-hybrid Homo sapiens
25 C14orf180  
Two-hybrid Homo sapiens
26 TMEM120B  
Two-hybrid Homo sapiens
27 ADGRB3 577
Two-hybrid Homo sapiens
28 TM6SF2  
Two-hybrid Homo sapiens
29 CCDC167  
Two-hybrid Homo sapiens
30 ZDHHC21  
Two-hybrid Homo sapiens
31 BMI1  
Reconstituted Complex Homo sapiens
32 C3orf52  
Two-hybrid Homo sapiens
33 NEU1 4758
Two-hybrid Homo sapiens
34 TMEM97 27346
Two-hybrid Homo sapiens
35 CLDN6 9074
Two-hybrid Homo sapiens
36 TNFSF9  
Affinity Capture-MS Homo sapiens
37 CCDC115 84317
Affinity Capture-MS Homo sapiens
38 PAQR6  
Two-hybrid Homo sapiens
39 CD68 968
Two-hybrid Homo sapiens
40 ERBB2 2064
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
41 ITGAM 3684
Two-hybrid Homo sapiens
42 Ctnnb1 12387
Affinity Capture-Western Mus musculus
43 GSK3B 2932
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
44 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 TMEM63B 55362
Affinity Capture-MS Homo sapiens
46 INSIG2  
Two-hybrid Homo sapiens
47 ACTA2 59
Affinity Capture-MS Homo sapiens
48 AQP1 358
Two-hybrid Homo sapiens
49 ERBB3 2065
Affinity Capture-Western Homo sapiens
50 EMP3 2014
Two-hybrid Homo sapiens
51 CYP4F2  
Two-hybrid Homo sapiens
52 LYN 4067
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
53 GALNT12 79695
Biochemical Activity Homo sapiens
54 CLDND2  
Two-hybrid Homo sapiens
55 TMEM187  
Two-hybrid Homo sapiens
56 VAMP2 6844
Affinity Capture-MS Homo sapiens
57 APOA2 336
Two-hybrid Homo sapiens
58 TMEM243  
Two-hybrid Homo sapiens
59 COL8A2 1296
Two-hybrid Homo sapiens
60 CLDN19  
Two-hybrid Homo sapiens
61 TMEM121  
Two-hybrid Homo sapiens
62 SCAMP5  
Two-hybrid Homo sapiens
63 TRAM1L1  
Two-hybrid Homo sapiens
64 RPL11 6135
Affinity Capture-MS Homo sapiens
65 TWIST1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
66 TMEM14C 51522
Two-hybrid Homo sapiens
67 OR10AG1  
Two-hybrid Homo sapiens
68 NINJ2  
Two-hybrid Homo sapiens
69 BCL2L2 599
Two-hybrid Homo sapiens
70 CYB5B 80777
Two-hybrid Homo sapiens
71 TMEM199  
Affinity Capture-MS Homo sapiens
72 IPO5 3843
Affinity Capture-Western Homo sapiens
73 EMC6 83460
Two-hybrid Homo sapiens
74 C14orf1 11161
Two-hybrid Homo sapiens
75 GPR108 56927
Two-hybrid Homo sapiens
76 PPARG 5468
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
77 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
78 GOLT1B 51026
Two-hybrid Homo sapiens
79 GAST  
Two-hybrid Homo sapiens
80 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
81 ICMT  
Two-hybrid Homo sapiens
82 ACTBL2 345651
Affinity Capture-MS Homo sapiens
83 ERBB4 2066
Affinity Capture-Western Homo sapiens
84 EGFR 1956
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
85 AQP3  
Two-hybrid Homo sapiens
86 TMEM67 91147
Affinity Capture-MS Homo sapiens
87 THBD 7056
Two-hybrid Homo sapiens
88 TBP  
Co-localization Homo sapiens
89 JUN 3725
Affinity Capture-Western Homo sapiens
90 TMEM86B  
Two-hybrid Homo sapiens
91 SERP2  
Two-hybrid Homo sapiens
92 RFT1 91869
Two-hybrid Homo sapiens
93 CXCL9  
Two-hybrid Homo sapiens
94 TMEM11 8834
Two-hybrid Homo sapiens
95 ENTPD3  
Two-hybrid Homo sapiens
96 NDUFA3  
Two-hybrid Homo sapiens
97 CACNG1  
Two-hybrid Homo sapiens
98 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 PPAPDC2  
Two-hybrid Homo sapiens
100 MIP  
Two-hybrid Homo sapiens
101 NKG7  
Two-hybrid Homo sapiens
102 EDDM3B  
Two-hybrid Homo sapiens
103 MALL  
Two-hybrid Homo sapiens
104 PPIF 10105
Two-hybrid Homo sapiens
105 C1GALT1 56913
Two-hybrid Homo sapiens
106 C2 717
Two-hybrid Homo sapiens
107 UNC50  
Two-hybrid Homo sapiens
108 ARV1  
Two-hybrid Homo sapiens
109 CMTM7  
Two-hybrid Homo sapiens
110 PLP1 5354
Two-hybrid Homo sapiens
111 GRB2 2885
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
112 JUP 3728
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
113 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
114 ADAM33 80332
Two-hybrid Homo sapiens
115 MAL2 114569
Two-hybrid Homo sapiens
116 CD47 961
Two-hybrid Homo sapiens
117 NINJ1 4814
Two-hybrid Homo sapiens
118 PNLIPRP1  
Two-hybrid Homo sapiens
119 SOS1 6654
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
120 BTN2A2  
Two-hybrid Homo sapiens
121 SLC38A7 55238
Two-hybrid Homo sapiens
122 SFTPC  
Two-hybrid Homo sapiens
123 TNFRSF10B 8795
Two-hybrid Homo sapiens
124 ADIPOQ 9370
Two-hybrid Homo sapiens
125 BRICD5  
Two-hybrid Homo sapiens
126 EZH2  
Reconstituted Complex Homo sapiens
127 SMCO4  
Two-hybrid Homo sapiens
128 VAMP5 10791
Two-hybrid Homo sapiens
129 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 TMEM229B  
Two-hybrid Homo sapiens
131 GDNF  
Two-hybrid Homo sapiens
132 CXCL16 58191
Two-hybrid Homo sapiens
133 ATP11C 286410
Affinity Capture-MS Homo sapiens
134 ANKRD46 157567
Two-hybrid Homo sapiens
135 IKBKB 3551
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
136 RHD  
Two-hybrid Homo sapiens
137 CTNND1 1500
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
138 IKBKG 8517
Affinity Capture-Western Homo sapiens
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