Experiment description of studies that identified EPHA4 in exosomes |
1 |
Experiment ID |
201 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22740476
|
Organism |
Homo sapiens |
Experiment description |
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. |
Authors |
"Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ." |
Journal name |
Electrophoresis
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
SW480 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
2 |
Experiment ID |
363 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
3 |
Experiment ID |
364 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
4 |
Experiment ID |
365 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
5 |
Experiment ID |
254 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|FLOT1|CD81
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
MNT-1 |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
6 |
Experiment ID |
255 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|FLOT1
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
G1 |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
7 |
Experiment ID |
256 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|FLOT1
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
501mel |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
8 |
Experiment ID |
257 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|FLOT1
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
Daju |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
9 |
Experiment ID |
259 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|FLOT1
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
A375M |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
10 |
Experiment ID |
260 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|FLOT1|CD81
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
1205Lu |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
11 |
Experiment ID |
211 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
cytochrome c|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
12 |
Experiment ID |
212 |
MISEV standards |
✔
CEM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
Cytochrome C|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|