Experiment ID |
65 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
HSP60|HSP27|GAPDH
|
EV Cytosolic markers |
✔
FLOT2
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19415654
|
Organism |
Homo sapiens |
Experiment description |
Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7. |
Authors |
Staubach S, Razawi H, Hanisch FG. |
Journal name |
PROTEOMICS
|
Publication year |
2009 |
Sample |
Breast cancer cells |
Sample name |
MCF-7 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting |