Gene description for SEMA4C
Gene name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
Gene symbol SEMA4C
Other names/aliases M-SEMA-F
SEMACL1
SEMAF
SEMAI
Species Homo sapiens
 Database cross references - SEMA4C
ExoCarta ExoCarta_54910
Vesiclepedia VP_54910
Entrez Gene 54910
HGNC 10731
MIM 604462
UniProt Q9C0C4  
 SEMA4C identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for SEMA4C
Molecular Function
    protein binding GO:0005515 IPI
    semaphorin receptor binding GO:0030215 IBA
    neuropilin binding GO:0038191 IBA
    chemorepellent activity GO:0045499 IBA
Biological Process
    neural crest cell migration GO:0001755 IBA
    neural tube closure GO:0001843 ISS
    axon guidance GO:0007411 IBA
    cell migration in hindbrain GO:0021535 ISS
    cerebellum development GO:0021549 IEA
    positive regulation of cell migration GO:0030335 IBA
    positive regulation of stress-activated MAPK cascade GO:0032874 IDA
    muscle cell differentiation GO:0042692 IDA
    negative chemotaxis GO:0050919 IBA
    semaphorin-plexin signaling pathway GO:0071526 IBA
    semaphorin-plexin signaling pathway GO:0071526 ISS
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    plasma membrane GO:0005886 IBA
    postsynaptic density GO:0014069 ISS
    synaptic vesicle membrane GO:0030672 IBA
    synaptic vesicle membrane GO:0030672 ISS
    postsynaptic density membrane GO:0098839 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified SEMA4C in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SEMA4C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Affinity Capture-MS Homo sapiens
2 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 OCLN 100506658
Proximity Label-MS Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 TSC2 7249
Affinity Capture-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 CAND2 23066
Affinity Capture-MS Homo sapiens
10 LGALS1 3956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 UBE4A 9354
Affinity Capture-MS Homo sapiens
12 SEMA4A  
Affinity Capture-MS Homo sapiens
13 STX4 6810
Proximity Label-MS Homo sapiens
14 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
15 TRIM54  
Two-hybrid Homo sapiens
16 RCHY1  
Two-hybrid Homo sapiens
17 TEX264 51368
Affinity Capture-MS Homo sapiens
18 HEATR3 55027
Affinity Capture-MS Homo sapiens
19 DCT 1638
Affinity Capture-MS Homo sapiens
20 RAB3B 5865
Proximity Label-MS Homo sapiens
21 KRT40  
Two-hybrid Homo sapiens
22 NBEAL2 23218
Affinity Capture-MS Homo sapiens
23 STAT3 6774
Affinity Capture-MS Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 KRT31 3881
Two-hybrid Homo sapiens
26 HELLS 3070
Affinity Capture-MS Homo sapiens
27 RHOB 388
Proximity Label-MS Homo sapiens
28 DLG2 1740
Two-hybrid Homo sapiens
29 FIGNL1 63979
Affinity Capture-MS Homo sapiens
30 BTAF1 9044
Affinity Capture-MS Homo sapiens
31 VPS16 64601
Affinity Capture-MS Homo sapiens
32 RAB35 11021
Proximity Label-MS Homo sapiens
33 IL13RA2 3598
Affinity Capture-MS Homo sapiens
34 EXOC7 23265
Affinity Capture-MS Homo sapiens
35 EXOC5 10640
Affinity Capture-MS Homo sapiens
36 LAMTOR1 55004
Proximity Label-MS Homo sapiens
37 SMC2 10592
Affinity Capture-MS Homo sapiens
38 TNF  
Affinity Capture-MS Homo sapiens
39 VSIG4  
Affinity Capture-MS Homo sapiens
40 HTR3C  
Affinity Capture-MS Homo sapiens
41 SYMPK 8189
Affinity Capture-MS Homo sapiens
42 ATP1B4  
Affinity Capture-MS Homo sapiens
43 PKM 5315
Affinity Capture-MS Homo sapiens
44 FAM114A2  
Affinity Capture-MS Homo sapiens
45 NUB1 51667
Affinity Capture-MS Homo sapiens
46 LYN 4067
Proximity Label-MS Homo sapiens
47 RAB4A 5867
Proximity Label-MS Homo sapiens
48 C12orf49  
Negative Genetic Homo sapiens
49 NCAPD3 23310
Affinity Capture-MS Homo sapiens
50 TNPO2 30000
Affinity Capture-MS Homo sapiens
51 DIRAS3  
Proximity Label-MS Homo sapiens
52 CYSRT1 375791
Two-hybrid Homo sapiens
53 SNX5 27131
Affinity Capture-MS Homo sapiens
54 EXOC6 54536
Affinity Capture-MS Homo sapiens
55 LRBA 987
Affinity Capture-MS Homo sapiens
56 GIPC2 54810
Affinity Capture-MS Homo sapiens
57 ELOVL5 60481
Proximity Label-MS Homo sapiens
58 VPS18 57617
Affinity Capture-MS Homo sapiens
59 COG4 25839
Affinity Capture-MS Homo sapiens
60 SEC61B 10952
Proximity Label-MS Homo sapiens
61 STAG2 10735
Affinity Capture-MS Homo sapiens
62 IKBKG 8517
Affinity Capture-MS Homo sapiens
63 UPK2 7379
Affinity Capture-MS Homo sapiens
64 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
65 CKAP5 9793
Affinity Capture-MS Homo sapiens
66 PLAA 9373
Affinity Capture-MS Homo sapiens
67 TMEM30A 55754
Affinity Capture-MS Homo sapiens
68 CANX 821
Affinity Capture-MS Homo sapiens
69 RAB5A 5868
Proximity Label-MS Homo sapiens
70 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SARM1 23098
Affinity Capture-MS Homo sapiens
72 ARVCF 421
Affinity Capture-MS Homo sapiens
73 MCOLN3  
Affinity Capture-MS Homo sapiens
74 ARFGEF1 10565
Affinity Capture-MS Homo sapiens
75 MZT2B 80097
Affinity Capture-MS Homo sapiens
76 HSD3B7 80270
Proximity Label-MS Homo sapiens
77 TNPO3 23534
Affinity Capture-MS Homo sapiens
78 YY1 7528
Affinity Capture-MS Homo sapiens
79 ARF6 382
Proximity Label-MS Homo sapiens
80 FBXO6 26270
Affinity Capture-MS Homo sapiens
81 ATP1B3 483
Affinity Capture-MS Homo sapiens
82 KIAA1524 57650
Affinity Capture-MS Homo sapiens
83 SNX27 81609
Affinity Capture-MS Homo sapiens
84 WAPAL  
Affinity Capture-MS Homo sapiens
85 SPACA1 81833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 KRT27 342574
Two-hybrid Homo sapiens
87 COG2 22796
Affinity Capture-MS Homo sapiens
88 XPO4 64328
Affinity Capture-MS Homo sapiens
89 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
90 DLG4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
91 FBXO2 26232
Affinity Capture-MS Homo sapiens
92 KIAA0391  
Affinity Capture-MS Homo sapiens
93 MTOR 2475
Affinity Capture-MS Homo sapiens
94 RAB9A 9367
Proximity Label-MS Homo sapiens
95 USHBP1  
Two-hybrid Homo sapiens
96 KRAS 3845
Proximity Label-MS Homo sapiens
97 PDSS1  
Affinity Capture-MS Homo sapiens
98 TBC1D9B 23061
Affinity Capture-MS Homo sapiens
99 CHUK 1147
Affinity Capture-MS Homo sapiens
100 ATP7A 538
Affinity Capture-MS Homo sapiens
101 KRT16 3868
Two-hybrid Homo sapiens
102 KRTAP6-3  
Two-hybrid Homo sapiens
103 HK2 3099
Affinity Capture-MS Homo sapiens
104 ZFAND2A  
Affinity Capture-MS Homo sapiens
105 PLXNB2 23654
Affinity Capture-MS Homo sapiens
106 MSTO1 55154
Affinity Capture-MS Homo sapiens
107 GIPC1 10755
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 KIF14 9928
Affinity Capture-MS Homo sapiens
109 DLG3 1741
Two-hybrid Homo sapiens
110 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
111 PEX19 5824
Affinity Capture-MS Homo sapiens
112 TTC28 23331
Affinity Capture-MS Homo sapiens
113 BUB1B  
Affinity Capture-MS Homo sapiens
114 NBPF19  
Two-hybrid Homo sapiens
115 TMEM30B 161291
Affinity Capture-MS Homo sapiens
116 DNAAF5 54919
Affinity Capture-MS Homo sapiens
117 GCLM 2730
Affinity Capture-MS Homo sapiens
118 TRIM9  
Affinity Capture-MS Homo sapiens
119 FCER1A  
Affinity Capture-MS Homo sapiens
120 PNMA1 9240
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
121 C11orf52 91894
Proximity Label-MS Homo sapiens
122 APOM 55937
Affinity Capture-MS Homo sapiens
123 ELMO2 63916
Affinity Capture-MS Homo sapiens
124 FLCN 201163
Affinity Capture-MS Homo sapiens
125 SFTPC  
Affinity Capture-MS Homo sapiens
126 KLHL42  
Affinity Capture-MS Homo sapiens
127 NF1 4763
Affinity Capture-MS Homo sapiens
128 BTBD1  
Affinity Capture-MS Homo sapiens
129 TBRG4 9238
Affinity Capture-MS Homo sapiens
130 SEC62 7095
Proximity Label-MS Homo sapiens
131 RAB7A 7879
Proximity Label-MS Homo sapiens
132 RAB11A 8766
Proximity Label-MS Homo sapiens
133 EPHA2 1969
Proximity Label-MS Homo sapiens
134 MYO6 4646
Proximity Label-MS Homo sapiens
135 RAB5C 5878
Proximity Label-MS Homo sapiens
136 HSF2BP  
Two-hybrid Homo sapiens
137 KRT34  
Two-hybrid Homo sapiens
138 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
139 PCDHB3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SEMA4C is involved
No pathways found





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