Gene description for CDCA3
Gene name
cell division cycle associated 3
Gene symbol
CDCA3
Other names/aliases
GRCC8 TOME-1
Species
Homo sapiens
Database cross references - CDCA3
ExoCarta
ExoCarta_83461
Entrez Gene
83461
HGNC
14624
MIM
607749
UniProt
Q99618
CDCA3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Prostate cancer cells
25844599
Gene ontology annotations for CDCA3
Experiment description of studies that identified CDCA3 in exosomes
1
Experiment ID
236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
Protein-protein interactions for CDCA3
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
WEE1
7465
Reconstituted Complex
Homo sapiens
2
TRAF2
Two-hybrid
Homo sapiens
3
CUL1
8454
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
4
CTDSP1
58190
Two-hybrid
Homo sapiens
5
SKP1
6500
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which CDCA3 is involved
No pathways found