Gene description for CDCA3
Gene name cell division cycle associated 3
Gene symbol CDCA3
Other names/aliases GRCC8
TOME-1
Species Homo sapiens
 Database cross references - CDCA3
ExoCarta ExoCarta_83461
Entrez Gene 83461
HGNC 14624
MIM 607749
UniProt Q99618  
 CDCA3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Prostate cancer cells 25844599    
 Gene ontology annotations for CDCA3
Molecular Function
    protein binding GO:0005515 IPI
    molecular_function GO:0003674 ND
Biological Process
    mitotic nuclear division GO:0007067 IEA
    protein ubiquitination GO:0016567 IEA
    cell division GO:0051301 IEA
    biological_process GO:0008150 ND
Subcellular Localization
    cellular_component GO:0005575 ND
    cytosol GO:0005829 IEA
 Experiment description of studies that identified CDCA3 in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for CDCA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WEE1 7465
Reconstituted Complex Homo sapiens
2 TRAF2  
Two-hybrid Homo sapiens
3 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 CTDSP1 58190
Two-hybrid Homo sapiens
5 SKP1 6500
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CDCA3 is involved
No pathways found





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