Gene description for SLC30A1
Gene name solute carrier family 30 (zinc transporter), member 1
Gene symbol SLC30A1
Other names/aliases ZNT1
ZRC1
Species Homo sapiens
 Database cross references - SLC30A1
ExoCarta ExoCarta_7779
Vesiclepedia VP_7779
Entrez Gene 7779
HGNC 11012
MIM 609521
UniProt Q9Y6M5  
 SLC30A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC30A1
Molecular Function
    zinc ion transmembrane transporter activity GO:0005385 IBA
    zinc ion transmembrane transporter activity GO:0005385 TAS
    protein binding GO:0005515 IPI
    calcium channel inhibitor activity GO:0019855 IBA
    calcium channel inhibitor activity GO:0019855 ISS
    metal ion binding GO:0046872 IEA
    zinc:proton antiporter activity GO:0140826 ISS
Biological Process
    in utero embryonic development GO:0001701 IEA
    zinc ion transport GO:0006829 ISS
    intracellular calcium ion homeostasis GO:0006874 ISS
    intracellular zinc ion homeostasis GO:0006882 IBA
    intracellular zinc ion homeostasis GO:0006882 IDA
    intracellular zinc ion homeostasis GO:0006882 ISS
    detoxification of zinc ion GO:0010312 IBA
    defense response to bacterium GO:0042742 IDA
    negative regulation of neurotransmitter secretion GO:0046929 ISS
    positive regulation of dendritic spine morphogenesis GO:0061003 IEA
    zinc ion import into organelle GO:0062111 IDA
    calcium ion import GO:0070509 IEA
    cadmium ion transmembrane transport GO:0070574 ISS
    zinc ion transmembrane transport GO:0071577 IBA
    zinc ion transmembrane transport GO:0071577 TAS
    negative regulation of zinc ion transmembrane import GO:0071584 ISS
    detoxification of cadmium ion GO:0071585 IEA
    negative regulation of calcium ion import GO:0090281 ISS
    zinc export across plasma membrane GO:0140882 ISS
    regulation of postsynaptic density protein 95 clustering GO:1902897 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IBA
    basolateral plasma membrane GO:0016323 IDA
    T-tubule GO:0030315 ISS
    dendrite GO:0030425 IEA
    cytoplasmic vesicle membrane GO:0030659 IDA
    nuclear membrane GO:0031965 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    postsynaptic density membrane GO:0098839 IEA
    postsynaptic density, intracellular component GO:0099092 IEA
    glutamatergic postsynaptic density GO:0099573 IEA
 Experiment description of studies that identified SLC30A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC30A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XXYLT1 152002
Affinity Capture-MS Homo sapiens
2 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 METTL9 51108
Affinity Capture-MS Homo sapiens
4 CLGN 1047
Affinity Capture-MS Homo sapiens
5 TMEM169  
Affinity Capture-MS Homo sapiens
6 FAM189B  
Affinity Capture-MS Homo sapiens
7 LGALS9 3965
Affinity Capture-MS Homo sapiens
8 RAB35 11021
Proximity Label-MS Homo sapiens
9 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 IL17RB  
Affinity Capture-MS Homo sapiens
11 SLC31A1 1317
Affinity Capture-MS Homo sapiens
12 EBAG9 9166
Proximity Label-MS Homo sapiens
13 PTH1R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 OCLN 100506658
Proximity Label-MS Homo sapiens
15 CANX 821
Affinity Capture-MS Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 MARCKS 4082
Proximity Label-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 XPO1 7514
Co-fractionation Homo sapiens
20 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 MTNR1B  
Two-hybrid Homo sapiens
22 GALNT6 11226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LAMP1 3916
Proximity Label-MS Homo sapiens
24 SRSF1 6426
Co-fractionation Homo sapiens
25 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CYBB 1536
Affinity Capture-MS Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 GJA1 2697
Proximity Label-MS Homo sapiens
29 GJD3  
Proximity Label-MS Homo sapiens
30 MTNR1A  
Two-hybrid Homo sapiens
31 LAMP2 3920
Proximity Label-MS Homo sapiens
32 TNFSF12 8742
Affinity Capture-MS Homo sapiens
33 ARF6 382
Proximity Label-MS Homo sapiens
34 GPR114  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CXADR 1525
Proximity Label-MS Homo sapiens
36 ERGIC1 57222
Proximity Label-MS Homo sapiens
37 C11orf52 91894
Proximity Label-MS Homo sapiens
38 LMAN1 3998
Proximity Label-MS Homo sapiens
39 LYN 4067
Proximity Label-MS Homo sapiens
40 PHLPP1  
Proximity Label-MS Homo sapiens
41 MCAM 4162
Proximity Label-MS Homo sapiens
42 RAB3B 5865
Proximity Label-MS Homo sapiens
43 RAB5A 5868
Proximity Label-MS Homo sapiens
44 TTYH1  
Affinity Capture-MS Homo sapiens
45 DNAJC5 80331
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 GALNT10 55568
Affinity Capture-MS Homo sapiens
47 CHRNA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TPST2 8459
Affinity Capture-MS Homo sapiens
49 CDH1 999
Proximity Label-MS Homo sapiens
50 EPHA2 1969
Proximity Label-MS Homo sapiens
51 DIRAS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DNAJC5B  
Proximity Label-MS Homo sapiens
53 PRPH2 5961
Affinity Capture-MS Homo sapiens
54 LGALS9B 284194
Affinity Capture-MS Homo sapiens
55 RAB9A 9367
Proximity Label-MS Homo sapiens
56 STS 412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 IL20RA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RAB2A 5862
Proximity Label-MS Homo sapiens
59 TGFA  
Affinity Capture-MS Homo sapiens
60 MARCH4  
Affinity Capture-MS Homo sapiens
61 KRAS 3845
Proximity Label-MS Homo sapiens
62 ATP6V1B1 525
Co-fractionation Homo sapiens
63 RHOB 388
Proximity Label-MS Homo sapiens
64 CDH16  
Affinity Capture-MS Homo sapiens
65 Bmpr1a  
Affinity Capture-MS Mus musculus
66 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 RAB5C 5878
Proximity Label-MS Homo sapiens
68 LMNA 4000
Proximity Label-MS Homo sapiens
69 CAV1 857
Proximity Label-MS Homo sapiens
70 CTDSPL 10217
Proximity Label-MS Homo sapiens
71 B3GALT5  
Affinity Capture-MS Homo sapiens
72 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 FLOT1 10211
Proximity Label-MS Homo sapiens
74 STX6 10228
Proximity Label-MS Homo sapiens
75 CLRN2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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