Gene description for IRS4
Gene name insulin receptor substrate 4
Gene symbol IRS4
Other names/aliases IRS-4
PY160
Species Homo sapiens
 Database cross references - IRS4
ExoCarta ExoCarta_8471
Entrez Gene 8471
HGNC 6128
MIM 300904
UniProt O14654  
 IRS4 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for IRS4
Molecular Function
    protein binding GO:0005515 IPI
    SH3/SH2 adaptor activity GO:0005070 TAS
    phosphatidylinositol 3-kinase binding GO:0043548 IBA
    insulin receptor binding GO:0005158 IBA
    signal transducer activity GO:0004871 IEA
Biological Process
    regulation of lipid metabolic process GO:0019216 IBA
    positive regulation of signal transduction GO:0009967 TAS
    insulin receptor signaling pathway GO:0008286 IBA
    signal transduction GO:0007165 TAS
Subcellular Localization
    cytosol GO:0005829 IBA
    intracellular membrane-bounded organelle GO:0043231 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified IRS4 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for IRS4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRK 1398
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 SOCS6  
Invitro Homo sapiens
3 PIK3R1 5295
Invivo Homo sapiens
4 PTPN11 5781
Invitro Homo sapiens
5 PRKCZ 5590
Invitro Homo sapiens
6 CRKL 1399
Reconstituted Complex Homo sapiens
7 GRB2 2885
Affinity Capture-Western Homo sapiens
8 NISCH 11188
Invivo Homo sapiens
9 PTPN6 5777
Invitro Homo sapiens
10 PIK3CA 5290
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which IRS4 is involved
PathwayEvidenceSource
IRS-related events triggered by IGF1R TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here