Gene description for AKT2
Gene name v-akt murine thymoma viral oncogene homolog 2
Gene symbol AKT2
Other names/aliases HIHGHH
PKBB
PKBBETA
PRKBB
RAC-BETA
Species Homo sapiens
 Database cross references - AKT2
ExoCarta ExoCarta_208
Entrez Gene 208
HGNC 392
MIM 164731
UniProt P31751  
 AKT2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for AKT2
Molecular Function
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    ATP binding GO:0005524 IDA
    kinase activity GO:0016301 TAS
Biological Process
    positive regulation of glucose metabolic process GO:0010907 IMP
    protein localization to plasma membrane GO:0072659 IEA
    positive regulation of fatty acid beta-oxidation GO:0032000 IMP
    positive regulation of protein targeting to membrane GO:0090314 ISS
    carbohydrate transport GO:0008643 IEA
    regulation of cell cycle arrest GO:0071156 TAS
    positive regulation of glucose import in response to insulin stimulus GO:2001275 IEA
    negative regulation of plasma membrane long-chain fatty acid transport GO:0010748 IMP
    glucose metabolic process GO:0006006 IEA
    regulation of cell migration GO:0030334 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    glycogen biosynthetic process GO:0005978 IEA
    regulation of translation GO:0006417 IEA
    apoptotic process GO:0006915 IEA
    cellular response to insulin stimulus GO:0032869 IMP
    positive regulation of protein phosphorylation GO:0001934 ISS
    peripheral nervous system myelin maintenance GO:0032287 IEA
    protein phosphorylation GO:0006468 IEA
    fat cell differentiation GO:0045444 TAS
    membrane organization GO:0061024 TAS
    positive regulation of cell motility GO:2000147 IMP
    positive regulation of glycogen biosynthetic process GO:0045725 IMP
    positive regulation of vesicle fusion GO:0031340 ISS
    cellular protein modification process GO:0006464 TAS
    intracellular protein transmembrane transport GO:0065002 ISS
    signal transduction GO:0007165 TAS
    positive regulation of glucose import GO:0046326 IMP
    mammary gland epithelial cell differentiation GO:0060644 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    ruffle membrane GO:0032587 ISS
    nucleus GO:0005634 TAS
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 ISS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified AKT2 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AKT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TCL1B  
Invivo Homo sapiens
Invitro Homo sapiens
2 TCL1A  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
3 SH3MD2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 CHUK 1147
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
5 GSK3B 2932
Co-crystal Structure Homo sapiens
6 APPL1 26060
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
7 PRKDC 5591
Biochemical Activity Homo sapiens
View the network image/svg+xml



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