Gene description for USP31
Gene name ubiquitin specific peptidase 31
Gene symbol USP31
Other names/aliases -
Species Homo sapiens
 Database cross references - USP31
ExoCarta ExoCarta_57478
Vesiclepedia VP_57478
Entrez Gene 57478
HGNC 20060
UniProt Q70CQ4  
 USP31 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for USP31
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IDA
Biological Process
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
 Experiment description of studies that identified USP31 in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for USP31
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Proximity Label-MS Homo sapiens
2 EPHA2 1969
Proximity Label-MS Homo sapiens
3 RELA 5970
Affinity Capture-Western Homo sapiens
4 KRAS 3845
Proximity Label-MS Homo sapiens
5 RHOB 388
Proximity Label-MS Homo sapiens
6 DNAJC5 80331
Proximity Label-MS Homo sapiens
7 SIRT1  
Affinity Capture-Western Homo sapiens
8 GAPDH 2597
Cross-Linking-MS (XL-MS) Homo sapiens
9 UBC 7316
Reconstituted Complex Homo sapiens
10 EGFR 1956
Affinity Capture-MS Homo sapiens
11 CAV1 857
Proximity Label-MS Homo sapiens
12 TRAF2 7186
Affinity Capture-Western Homo sapiens
13 CTDSPL 10217
Proximity Label-MS Homo sapiens
14 HSP90AA1 3320
Cross-Linking-MS (XL-MS) Homo sapiens
15 OCLN 100506658
Proximity Label-MS Homo sapiens
16 C11orf52 91894
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which USP31 is involved
No pathways found





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