Gene description for PES1
Gene name pescadillo ribosomal biogenesis factor 1
Gene symbol PES1
Other names/aliases PES
Species Homo sapiens
 Database cross references - PES1
ExoCarta ExoCarta_23481
Vesiclepedia VP_23481
Entrez Gene 23481
HGNC 8848
MIM 605819
UniProt O00541  
 PES1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PES1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    ribonucleoprotein complex binding GO:0043021 IEA
Biological Process
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IBA
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IMP
    maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000466 IMP
    rRNA processing GO:0006364 IMP
    cell population proliferation GO:0008283 IMP
    ribosomal large subunit biogenesis GO:0042273 IC
    regulation of cell cycle GO:0051726 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    preribosome, large subunit precursor GO:0030687 IDA
    PeBoW complex GO:0070545 IBA
    PeBoW complex GO:0070545 IDA
 Experiment description of studies that identified PES1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
17
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
18
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
19
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
20
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
21
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PES1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CDC14B 8555
Proximity Label-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 ORC2  
Co-fractionation Homo sapiens
5 AFF4 27125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CENPB 1059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NOLC1 9221
Co-fractionation Homo sapiens
8 DNAJC8 22826
Proximity Label-MS Homo sapiens
9 KIAA0020 9933
Co-fractionation Homo sapiens
10 PPIL4  
Affinity Capture-MS Homo sapiens
11 TNRC18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 NOP56 10528
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
13 ESF1 51575
Co-fractionation Homo sapiens
14 BRD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
16 NHP2L1 4809
Co-fractionation Homo sapiens
17 CAND1 55832
Affinity Capture-MS Homo sapiens
18 KLHL23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PRPF40A 55660
Affinity Capture-MS Homo sapiens
20 SOX2  
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
22 CENPA  
Proximity Label-MS Homo sapiens
23 DNAJB6 10049
Proximity Label-MS Homo sapiens
24 APEX1 328
Proximity Label-MS Homo sapiens
25 TCEB3 6924
Affinity Capture-MS Homo sapiens
26 DDX23 9416
Proximity Label-MS Homo sapiens
27 PARK2  
Affinity Capture-MS Homo sapiens
28 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PRR11  
Affinity Capture-MS Homo sapiens
30 KIF23 9493
Affinity Capture-MS Homo sapiens
31 DDX24 57062
Co-fractionation Homo sapiens
32 C18orf25 147339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RRP12 23223
Affinity Capture-MS Homo sapiens
34 FBXW7  
Affinity Capture-MS Homo sapiens
35 PRC1 9055
Affinity Capture-MS Homo sapiens
36 NPM1 4869
Affinity Capture-MS Homo sapiens
37 SRRM2 23524
Affinity Capture-MS Homo sapiens
38 BUB3 9184
Affinity Capture-MS Homo sapiens
39 WDR12 55759
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RPF2 84154
Co-fractionation Homo sapiens
42 DDX18 8886
Affinity Capture-MS Homo sapiens
43 FBL 2091
Co-fractionation Homo sapiens
44 NAP1L4 4676
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
45 MECP2 4204
Affinity Capture-MS Homo sapiens
46 PFKFB3 5209
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MYCN  
Affinity Capture-MS Homo sapiens
48 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 KIF14 9928
Affinity Capture-MS Homo sapiens
51 PES1 23481
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
52 LZTFL1 54585
Co-fractionation Homo sapiens
53 KLF16  
Affinity Capture-MS Homo sapiens
54 CXXC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PWP1 11137
Co-fractionation Homo sapiens
56 UTP14A 10813
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
57 TRIM26 7726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 MAGEA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RPL4 6124
Co-fractionation Homo sapiens
60 RBM28 55131
Co-fractionation Homo sapiens
61 DRG1 4733
Affinity Capture-MS Homo sapiens
62 LYN 4067
Proximity Label-MS Homo sapiens
63 NOP2 4839
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
64 NAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RPL18A 6142
Co-fractionation Homo sapiens
66 Srp72  
Affinity Capture-MS Mus musculus
67 SREBF2 6721
Positive Genetic Homo sapiens
68 MYH9 4627
Affinity Capture-MS Homo sapiens
69 NTRK1 4914
Affinity Capture-MS Homo sapiens
70 RPL31 6160
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
71 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 NCL 4691
Affinity Capture-MS Homo sapiens
73 RPL36 25873
Affinity Capture-MS Homo sapiens
74 RPL27A 6157
Co-fractionation Homo sapiens
75 UBTF 7343
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FOXL2  
Affinity Capture-MS Homo sapiens
77 RRP1B 23076
Affinity Capture-MS Homo sapiens
78 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
79 DDX27 55661
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 CEBPA  
Protein-peptide Homo sapiens
81 GTPBP4 23560
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
82 GNL3L 54552
Co-fractionation Homo sapiens
83 CD3EAP  
Proximity Label-MS Homo sapiens
84 SCARNA22  
Affinity Capture-RNA Homo sapiens
85 ANLN 54443
Affinity Capture-MS Homo sapiens
86 AIM2  
Affinity Capture-MS Homo sapiens
87 FANCD2  
Affinity Capture-MS Homo sapiens
88 NOC3L 64318
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 RPL23 9349
Co-fractionation Homo sapiens
90 MRTO4 51154
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
91 POP1 10940
Affinity Capture-MS Homo sapiens
92 LMNB1 4001
Affinity Capture-MS Homo sapiens
93 RC3H2  
Affinity Capture-MS Homo sapiens
94 SIRT7  
Affinity Capture-MS Homo sapiens
95 NOC4L 79050
Co-fractionation Homo sapiens
96 IGKC 3514
Affinity Capture-MS Homo sapiens
97 NPM3 10360
Affinity Capture-MS Homo sapiens
98 CUL3 8452
Affinity Capture-MS Homo sapiens
99 Rrbp1  
Affinity Capture-MS Mus musculus
100 DCAF13 25879
Affinity Capture-MS Homo sapiens
101 WDR55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 PDIA3 2923
Affinity Capture-MS Homo sapiens
103 EBNA1BP2 10969
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
104 CCDC91 55297
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 NAP1L1 4673
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
106 RPL7L1 285855
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 ARL2 402
Co-fractionation Homo sapiens
108 NIP7 51388
Co-fractionation Homo sapiens
109 NXF1 10482
Affinity Capture-RNA Homo sapiens
Affinity Capture-MS Homo sapiens
110 XPO6 23214
Affinity Capture-MS Homo sapiens
111 LOC100132735  
Protein-RNA Homo sapiens
112 MKRN1 23608
Affinity Capture-MS Homo sapiens
113 INPP5K 51763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 DKC1 1736
Affinity Capture-MS Homo sapiens
115 MYC  
Dosage Lethality Homo sapiens
Affinity Capture-MS Homo sapiens
116 RPA3 6119
Proximity Label-MS Homo sapiens
117 CEBPZ  
Co-fractionation Homo sapiens
118 DNAJC9 23234
Proximity Label-MS Homo sapiens
119 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
120 TOP1 7150
Affinity Capture-MS Homo sapiens
121 BLMH 642
Co-fractionation Homo sapiens
122 DDRGK1 65992
Affinity Capture-MS Homo sapiens
123 RNF2  
Affinity Capture-MS Homo sapiens
124 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
125 RPL7 6129
Co-fractionation Homo sapiens
126 FSCN1 6624
Affinity Capture-MS Homo sapiens
127 FTSJ3 117246
Co-fractionation Homo sapiens
128 SUPT16H 11198
Co-fractionation Homo sapiens
129 GNL3 26354
Co-fractionation Homo sapiens
130 RRBP1 6238
Affinity Capture-MS Homo sapiens
131 SART1 9092
Affinity Capture-MS Homo sapiens
132 DHX15 1665
Co-fractionation Homo sapiens
133 KIF2A 3796
Affinity Capture-MS Homo sapiens
134 ARRB2 409
Affinity Capture-MS Homo sapiens
135 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
136 FASN 2194
Negative Genetic Homo sapiens
137 AP3B1 8546
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 PARP1 142
Proximity Label-MS Homo sapiens
139 UBE2O 63893
Co-fractionation Homo sapiens
140 FBXL6  
Affinity Capture-MS Homo sapiens
141 TP53 7157
Affinity Capture-MS Homo sapiens
142 RRS1 23212
Affinity Capture-MS Homo sapiens
143 MNDA 4332
Affinity Capture-MS Homo sapiens
144 DDX3X 1654
Affinity Capture-MS Homo sapiens
145 USP36  
Affinity Capture-MS Homo sapiens
146 BOP1 23246
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 UBA52 7311
Co-fractionation Homo sapiens
148 G3BP1 10146
Affinity Capture-MS Homo sapiens
149 C1orf35  
Affinity Capture-MS Homo sapiens
150 NSA2  
Co-fractionation Homo sapiens
151 GNL1 2794
Co-fractionation Homo sapiens
152 SIRT1  
Co-fractionation Homo sapiens
153 MKI67  
Affinity Capture-MS Homo sapiens
154 NIFK 84365
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
155 BRIX1 55299
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
156 SETD1A 9739
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 TLR9  
Affinity Capture-MS Homo sapiens
158 UFL1 23376
Affinity Capture-MS Homo sapiens
159 KRR1 11103
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
160 NOC2L 26155
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
161 RPL38 6169
Co-fractionation Homo sapiens
162 RPS24 6229
Proximity Label-MS Homo sapiens
163 MOV10 4343
Affinity Capture-RNA Homo sapiens
164 TRIM23 373
Affinity Capture-Western Homo sapiens
165 ARRB1 408
Affinity Capture-MS Homo sapiens
166 RPL7A 6130
Co-fractionation Homo sapiens
167 RPS6 6194
Proximity Label-MS Homo sapiens
168 RPL8 6132
Co-fractionation Homo sapiens
169 RPL27 6155
Co-fractionation Homo sapiens
170 FOXM1  
Affinity Capture-MS Homo sapiens
171 MLLT3 4300
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
172 SERBP1 26135
Affinity Capture-MS Homo sapiens
173 CUL7 9820
Affinity Capture-MS Homo sapiens
174 KRAS 3845
Negative Genetic Homo sapiens
175 C9orf72  
Affinity Capture-MS Homo sapiens
176 CWC22 57703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 RC3H1 149041
Affinity Capture-MS Homo sapiens
178 PTRF 284119
Co-fractionation Homo sapiens
179 NVL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
181 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here