Gene description for EPHA3
Gene name EPH receptor A3
Gene symbol EPHA3
Other names/aliases EK4
ETK
ETK1
HEK
HEK4
TYRO4
Species Homo sapiens
 Database cross references - EPHA3
ExoCarta ExoCarta_2042
Entrez Gene 2042
HGNC 3387
MIM 179611
UniProt P29320  
 EPHA3 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for EPHA3
Molecular Function
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    GPI-linked ephrin receptor activity GO:0005004 IDA
Biological Process
    fasciculation of sensory neuron axon GO:0097155 ISS
    ephrin receptor signaling pathway GO:0048013 TAS
    axon guidance GO:0007411 TAS
    cell adhesion GO:0007155 IEA
    regulation of GTPase activity GO:0043087 IDA
    fasciculation of motor neuron axon GO:0097156 ISS
    positive regulation of neuron projection development GO:0010976 IMP
    cell migration GO:0016477 ISS
    regulation of actin cytoskeleton organization GO:0032956 IDA
    regulation of focal adhesion assembly GO:0051893 IDA
    regulation of microtubule cytoskeleton organization GO:0070507 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    cellular response to retinoic acid GO:0071300 IMP
    regulation of epithelial to mesenchymal transition GO:0010717 ISS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 IDA
    extracellular region GO:0005576 IEA
    early endosome GO:0005769 IDA
 Experiment description of studies that identified EPHA3 in exosomes
1
Experiment ID 255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for EPHA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EFNA5  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 CRK 1398
Affinity Capture-Western Homo sapiens
3 RUFY2  
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 EFNB2 1948
Reconstituted Complex Homo sapiens
5 RUFY1 80230
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 TP53  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which EPHA3 is involved
PathwayEvidenceSource
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
EPHA-mediated growth cone collapse TAS Reactome





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