Gene description for CD22
Gene name CD22 molecule
Gene symbol CD22
Other names/aliases SIGLEC-2
SIGLEC2
Species Homo sapiens
 Database cross references - CD22
ExoCarta ExoCarta_933
Vesiclepedia VP_933
Entrez Gene 933
HGNC 1643
MIM 107266
UniProt P20273  
 CD22 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
 Gene ontology annotations for CD22
Molecular Function
    IgM binding GO:0001791 TAS
    signaling receptor binding GO:0005102 TAS
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IBA
    protein phosphatase binding GO:0019903 ISS
    protein phosphatase binding GO:0019903 TAS
    carbohydrate binding GO:0030246 IEA
    sialic acid binding GO:0033691 IBA
    sialic acid binding GO:0033691 ISS
    sialic acid binding GO:0033691 TAS
    CD4 receptor binding GO:0042609 IBA
    CD4 receptor binding GO:0042609 ISS
Biological Process
    negative regulation of immunoglobulin production GO:0002638 ISS
    cell adhesion GO:0007155 IEA
    regulation of endocytosis GO:0030100 IDA
    regulation of B cell proliferation GO:0030888 IBA
    regulation of B cell proliferation GO:0030888 ISS
    B cell activation GO:0042113 IBA
    B cell activation GO:0042113 IDA
    regulation of immune response GO:0050776 ISS
    negative regulation of calcium-mediated signaling GO:0050849 ISS
    negative regulation of B cell receptor signaling pathway GO:0050859 IBA
    negative regulation of B cell receptor signaling pathway GO:0050859 IDA
    negative regulation of B cell receptor signaling pathway GO:0050859 IMP
    negative regulation of B cell receptor signaling pathway GO:0050859 TAS
Subcellular Localization
    cytoplasm GO:0005737 TAS
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 ISS
    membrane GO:0016020 NAS
    neuronal cell body membrane GO:0032809 ISS
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CD22 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
 Protein-protein interactions for CD22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
2 MCAM 4162
Proximity Label-MS Homo sapiens
3 AP2M1 1173
Two-hybrid Homo sapiens
4 ATG16L1 55054
Affinity Capture-MS Homo sapiens
5 SYK 6850
Affinity Capture-Western Homo sapiens
6 INPP5D 3635
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 S100A7 6278
Affinity Capture-MS Homo sapiens
8 PLCG1 5335
Affinity Capture-Western Homo sapiens
9 CPA4 51200
Affinity Capture-MS Homo sapiens
10 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 PTPRC 5788
Affinity Capture-Western Homo sapiens
13 CD19 930
Affinity Capture-Western Homo sapiens
14 PTPN6 5777
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 AP2A1 160
Affinity Capture-Western Homo sapiens
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