Gene description for CDC42EP1
Gene name CDC42 effector protein (Rho GTPase binding) 1
Gene symbol CDC42EP1
Other names/aliases BORG5
CEP1
MSE55
Species Homo sapiens
 Database cross references - CDC42EP1
ExoCarta ExoCarta_11135
Vesiclepedia VP_11135
Entrez Gene 11135
HGNC 17014
MIM 606084
UniProt Q00587  
 CDC42EP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CDC42EP1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
    cadherin binding involved in cell-cell adhesion GO:0098641 HDA
Biological Process
    Rho protein signal transduction GO:0007266 IBA
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 IDA
    positive regulation of actin filament polymerization GO:0030838 IBA
    positive regulation of pseudopodium assembly GO:0031274 IBA
    positive regulation of pseudopodium assembly GO:0031274 IDA
    cell-cell adhesion GO:0098609 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    adherens junction GO:0005912 HDA
    focal adhesion GO:0005925 HDA
    endomembrane system GO:0012505 IEA
 Experiment description of studies that identified CDC42EP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDC42EP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPRTN  
Affinity Capture-MS Homo sapiens
2 RBPMS 11030
Two-hybrid Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 DEF6 50619
Two-hybrid Homo sapiens
5 FAM167A  
Affinity Capture-MS Homo sapiens
6 MARCKS 4082
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 PLSCR1 5359
Two-hybrid Homo sapiens
9 LAMP2 3920
Proximity Label-MS Homo sapiens
10 TAF1  
Affinity Capture-MS Homo sapiens
11 ARMC7  
Two-hybrid Homo sapiens
12 CELA3A  
Affinity Capture-MS Homo sapiens
13 CAPZB 832
Affinity Capture-MS Homo sapiens
14 KRTAP4-12  
Two-hybrid Homo sapiens
15 FCN3 8547
Affinity Capture-MS Homo sapiens
16 FAM174A 345757
Affinity Capture-MS Homo sapiens
17 STX7 8417
Proximity Label-MS Homo sapiens
18 GPR55  
Affinity Capture-MS Homo sapiens
19 KLHL33  
Affinity Capture-MS Homo sapiens
20 RHOB 388
Proximity Label-MS Homo sapiens
21 RHOJ 57381
Two-hybrid Homo sapiens
22 FAM96B 51647
Affinity Capture-MS Homo sapiens
23 RAB35 11021
Proximity Label-MS Homo sapiens
24 NPAS1  
Affinity Capture-MS Homo sapiens
25 FAM20C 56975
Affinity Capture-MS Homo sapiens
26 DHDH  
Affinity Capture-MS Homo sapiens
27 LAMTOR1 55004
Proximity Label-MS Homo sapiens
28 YWHAB 7529
Affinity Capture-MS Homo sapiens
29 E2F4  
Affinity Capture-MS Homo sapiens
30 C2orf27B  
Affinity Capture-MS Homo sapiens
31 FHL3 2275
Two-hybrid Homo sapiens
32 ANTXR1 84168
Affinity Capture-MS Homo sapiens
33 CXADR 1525
Proximity Label-MS Homo sapiens
34 LYN 4067
Proximity Label-MS Homo sapiens
35 MCAM 4162
Proximity Label-MS Homo sapiens
36 VSIG4  
Affinity Capture-MS Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 TUBB2B 347733
Affinity Capture-MS Homo sapiens
39 CDH1 999
Proximity Label-MS Homo sapiens
40 DIRAS3  
Proximity Label-MS Homo sapiens
41 VCP 7415
Affinity Capture-MS Homo sapiens
42 OGT 8473
Reconstituted Complex Homo sapiens
43 AHNAK 79026
Affinity Capture-MS Homo sapiens
44 F12  
Affinity Capture-MS Homo sapiens
45 CLEC11A 6320
Affinity Capture-MS Homo sapiens
46 SAT1  
Two-hybrid Homo sapiens
47 CTDSPL 10217
Proximity Label-MS Homo sapiens
48 DNAJB11 51726
Affinity Capture-MS Homo sapiens
49 UBXN6 80700
Affinity Capture-MS Homo sapiens
50 SP100 6672
Affinity Capture-MS Homo sapiens
51 LY86  
Affinity Capture-MS Homo sapiens
52 KLK5 25818
Affinity Capture-MS Homo sapiens
53 XPO1 7514
Affinity Capture-MS Homo sapiens
54 LAMP1 3916
Proximity Label-MS Homo sapiens
55 PRKAA2 5563
Two-hybrid Homo sapiens
56 MGARP  
Affinity Capture-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
58 TUBB4B 10383
Affinity Capture-MS Homo sapiens
59 ARF6 382
Proximity Label-MS Homo sapiens
60 METTL21B  
Affinity Capture-MS Homo sapiens
61 RIPK4  
Affinity Capture-MS Homo sapiens
62 CDC42 998
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
63 AURKB 9212
Affinity Capture-MS Homo sapiens
64 CDK20  
Affinity Capture-MS Homo sapiens
65 Kctd5  
Affinity Capture-MS Mus musculus
66 LYZL2  
Affinity Capture-MS Homo sapiens
67 TUBB2A 7280
Affinity Capture-MS Homo sapiens
68 STK16 8576
Affinity Capture-MS Homo sapiens
69 MAPKAP1 79109
Two-hybrid Homo sapiens
70 CA10  
Affinity Capture-MS Homo sapiens
71 KIF14 9928
Affinity Capture-MS Homo sapiens
72 DNAJA2 10294
Affinity Capture-MS Homo sapiens
73 COMMD5 28991
Affinity Capture-MS Homo sapiens
74 TNFSF14  
Affinity Capture-MS Homo sapiens
75 SEPT10 151011
Proximity Label-MS Homo sapiens
76 SFTPA2  
Affinity Capture-MS Homo sapiens
77 CAV1 857
Proximity Label-MS Homo sapiens
78 ERGIC1 57222
Proximity Label-MS Homo sapiens
79 C11orf52 91894
Proximity Label-MS Homo sapiens
80 TSHR 7253
Affinity Capture-MS Homo sapiens
81 RAB2A 5862
Proximity Label-MS Homo sapiens
82 BRD1 23774
Affinity Capture-MS Homo sapiens
83 EPHA2 1969
Proximity Label-MS Homo sapiens
84 KRAS 3845
Proximity Label-MS Homo sapiens
85 RAB5C 5878
Proximity Label-MS Homo sapiens
86 C9orf72  
Affinity Capture-MS Homo sapiens
87 KBTBD4  
Affinity Capture-MS Homo sapiens
88 TIMP3 7078
Affinity Capture-MS Homo sapiens
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