Gene description for NISCH
Gene name nischarin
Gene symbol NISCH
Other names/aliases I-1
IR1
IRAS
hIRAS
Species Homo sapiens
 Database cross references - NISCH
ExoCarta ExoCarta_11188
Entrez Gene 11188
HGNC 18006
MIM 615507
UniProt Q9Y2I1  
 NISCH identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for NISCH
Molecular Function
    protein binding GO:0005515 IPI
    G-protein coupled amine receptor activity GO:0008227 IEA
    integrin binding GO:0005178 IEA
    identical protein binding GO:0042802 IPI
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    glucose metabolic process GO:0006006 IEA
    negative regulation of cell migration GO:0030336 IEA
    Rac protein signal transduction GO:0016601 IEA
    regulation of blood pressure GO:0008217 IEA
    actin cytoskeleton organization GO:0030036 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    apoptotic process GO:0006915 IEA
    norepinephrine secretion GO:0048243 IEA
    regulation of synaptic transmission, GABAergic GO:0032228 IEA
Subcellular Localization
    early endosome GO:0005769 IEA
    recycling endosome GO:0055037 IEA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified NISCH in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for NISCH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IRS4 8471
Invivo Homo sapiens
2 AR 367
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which NISCH is involved
No pathways found





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