Gene description for NUMBL
Gene name numb homolog (Drosophila)-like
Gene symbol NUMBL
Other names/aliases CAG3A
CTG3a
NBL
NUMB-R
NUMBLIKE
NUMBR
TNRC23
Species Homo sapiens
 Database cross references - NUMBL
ExoCarta ExoCarta_9253
Vesiclepedia VP_9253
Entrez Gene 9253
HGNC 8061
MIM 604018
UniProt Q9Y6R0  
 NUMBL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NUMBL
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    nervous system development GO:0007399 TAS
    axonogenesis GO:0007409 IEA
    cytokine-mediated signaling pathway GO:0019221 IDA
    protein metabolic process GO:0019538 IDA
    lateral ventricle development GO:0021670 ISS
    neuroblast division in subventricular zone GO:0021849 ISS
    adherens junction organization GO:0034332 IEA
    positive regulation of neurogenesis GO:0050769 IBA
    positive regulation of neurogenesis GO:0050769 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified NUMBL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NUMBL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
3 STX6 10228
Proximity Label-MS Homo sapiens
4 ATXN1 6310
Two-hybrid Homo sapiens
5 GTSE1 51512
Affinity Capture-MS Homo sapiens
6 LIMA1 51474
Affinity Capture-MS Homo sapiens
7 GJD3  
Proximity Label-MS Homo sapiens
8 ACTN1 87
Two-hybrid Homo sapiens
9 RAB35 11021
Proximity Label-MS Homo sapiens
10 THPO  
Two-hybrid Homo sapiens
11 AP2B1 163
Proximity Label-MS Homo sapiens
12 PSMA3 5684
Two-hybrid Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 MARCKS 4082
Proximity Label-MS Homo sapiens
15 KIAA1107  
Affinity Capture-MS Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 C11orf52 91894
Proximity Label-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 XPO1 7514
Affinity Capture-MS Homo sapiens
20 GJA1 2697
Proximity Label-MS Homo sapiens
21 SPSB3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 PFN1 5216
Proximity Label-MS Homo sapiens
23 RAB11A 8766
Proximity Label-MS Homo sapiens
24 EZR 7430
Proximity Label-MS Homo sapiens
25 RIPK1 8737
Two-hybrid Homo sapiens
26 TAB2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
27 LCK 3932
Proximity Label-MS Homo sapiens
28 TRAF6 7189
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
29 UBE3A 7337
Reconstituted Complex Homo sapiens
30 ARF6 382
Proximity Label-MS Homo sapiens
31 BHLHE40  
Two-hybrid Homo sapiens
32 EGFR 1956
Protein-peptide Homo sapiens
33 GAA 2548
Two-hybrid Homo sapiens
34 Eps15l1  
Protein-peptide Mus musculus
35 CXADR 1525
Proximity Label-MS Homo sapiens
36 CLTB 1212
Proximity Label-MS Homo sapiens
37 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 OCLN 100506658
Proximity Label-MS Homo sapiens
39 LYN 4067
Proximity Label-MS Homo sapiens
40 DNAJC5 80331
Proximity Label-MS Homo sapiens
41 RAB4A 5867
Proximity Label-MS Homo sapiens
42 MCAM 4162
Proximity Label-MS Homo sapiens
43 RBFOX1 54715
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
44 C10orf88  
Affinity Capture-MS Homo sapiens
45 MAGED1 9500
Two-hybrid Homo sapiens
46 CDH1 999
Proximity Label-MS Homo sapiens
47 YWHAZ 7534
Affinity Capture-MS Homo sapiens
48 NUMB 8650
Affinity Capture-MS Homo sapiens
49 DIRAS3  
Proximity Label-MS Homo sapiens
50 SIAH1  
Two-hybrid Homo sapiens
51 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CLTA 1211
Proximity Label-MS Homo sapiens
53 TRAF5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
54 RAB3IP  
Affinity Capture-MS Homo sapiens
55 PEX14 5195
Proximity Label-MS Homo sapiens
56 ESRP2 80004
Two-hybrid Homo sapiens
57 Eps15 13858
Protein-peptide Mus musculus
Reconstituted Complex Mus musculus
58 GPATCH8  
Two-hybrid Homo sapiens
59 ACTB 60
Proximity Label-MS Homo sapiens
60 DDRGK1 65992
Affinity Capture-MS Homo sapiens
61 UNC5CL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 KRAS 3845
Proximity Label-MS Homo sapiens
63 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SLC1A1 6505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 TMC5 79838
Two-hybrid Homo sapiens
66 CAV1 857
Proximity Label-MS Homo sapiens
67 GRIP1  
Two-hybrid Homo sapiens
68 CTDSPL 10217
Proximity Label-MS Homo sapiens
69 CBY1  
Affinity Capture-MS Homo sapiens
70 FLOT1 10211
Proximity Label-MS Homo sapiens
71 MECP2 4204
Affinity Capture-MS Homo sapiens
72 TULP3 7289
Affinity Capture-MS Homo sapiens
73 RAB9A 9367
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUMBL is involved
No pathways found





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