Gene description for PI4KA
Gene name phosphatidylinositol 4-kinase, catalytic, alpha
Gene symbol PI4KA
Other names/aliases PI4K-ALPHA
PIK4CA
pi4K230
Species Homo sapiens
 Database cross references - PI4KA
ExoCarta ExoCarta_5297
Vesiclepedia VP_5297
Entrez Gene 5297
HGNC 8983
MIM 600286
 PI4KA identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for PI4KA
Molecular Function
    1-phosphatidylinositol 4-kinase activity GO:0004430 IBA
    1-phosphatidylinositol 4-kinase activity GO:0004430 ISS
    1-phosphatidylinositol 4-kinase activity GO:0004430 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cadherin binding GO:0045296 HDA
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    signal transduction GO:0007165 NAS
    modulation by host of viral process GO:0044788 IMP
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 ISS
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    reorganization of cellular membranes to establish viral sites of replication GO:0140754 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 HDA
    membrane GO:0016020 HDA
    Golgi-associated vesicle membrane GO:0030660 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PI4KA in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
13
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PI4KA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 C5AR2  
Affinity Capture-MS Homo sapiens
4 OCLN 100506658
Proximity Label-MS Homo sapiens
5 MARCKS 4082
Proximity Label-MS Homo sapiens
6 KLK5 25818
Affinity Capture-MS Homo sapiens
7 GJA1 2697
Proximity Label-MS Homo sapiens
8 ADH7  
Affinity Capture-MS Homo sapiens
9 EFR3A 23167
Affinity Capture-MS Homo sapiens
10 POF1B 79983
Affinity Capture-MS Homo sapiens
11 SERPINB13 5275
Affinity Capture-MS Homo sapiens
12 C2orf50  
Affinity Capture-MS Homo sapiens
13 FPR1  
Affinity Capture-MS Homo sapiens
14 NDUFA4 4697
Affinity Capture-MS Homo sapiens
15 SOX2  
Affinity Capture-MS Homo sapiens
16 HAL 3034
Affinity Capture-MS Homo sapiens
17 FAM126A 84668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 S1PR4  
Affinity Capture-MS Homo sapiens
19 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PPP3CB  
Affinity Capture-MS Homo sapiens
22 DUSP14 11072
Affinity Capture-MS Homo sapiens
23 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GPR55  
Affinity Capture-MS Homo sapiens
25 IVL 3713
Affinity Capture-MS Homo sapiens
26 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FLG 2312
Affinity Capture-MS Homo sapiens
28 RHOB 388
Proximity Label-MS Homo sapiens
29 RGS20 8601
Affinity Capture-MS Homo sapiens
30 ITGA4 3676
Affinity Capture-MS Homo sapiens
31 DGCR2 9993
Affinity Capture-MS Homo sapiens
32 DNAJC7 7266
Proximity Label-MS Homo sapiens
33 VCAM1 7412
Affinity Capture-MS Homo sapiens
34 F2RL1  
Affinity Capture-MS Homo sapiens
35 RAB35 11021
Proximity Label-MS Homo sapiens
36 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 EBAG9 9166
Proximity Label-MS Homo sapiens
38 C9orf163  
Affinity Capture-MS Homo sapiens
39 KIF14 9928
Affinity Capture-MS Homo sapiens
40 EFR3B  
Affinity Capture-MS Homo sapiens
41 PROKR1  
Affinity Capture-MS Homo sapiens
42 MTNR1B  
Reconstituted Complex Homo sapiens
43 UBR4 23352
Affinity Capture-MS Homo sapiens
44 SULT1C4  
Affinity Capture-MS Homo sapiens
45 DNAJB8 165721
Affinity Capture-MS Homo sapiens
46 DSG1 1828
Affinity Capture-MS Homo sapiens
47 CD79B 974
Affinity Capture-MS Homo sapiens
48 PLBD1 79887
Affinity Capture-MS Homo sapiens
49 SBSN 374897
Affinity Capture-MS Homo sapiens
50 P2RY8  
Affinity Capture-MS Homo sapiens
51 LYN 4067
Proximity Label-MS Homo sapiens
52 ILVBL 10994
Affinity Capture-MS Homo sapiens
53 BAG6 7917
Affinity Capture-MS Homo sapiens
54 VSIG4  
Affinity Capture-MS Homo sapiens
55 CRYAB 1410
Affinity Capture-MS Homo sapiens
56 FAM126B  
Affinity Capture-MS Homo sapiens
57 DNAJC5 80331
Proximity Label-MS Homo sapiens
58 CDH1 999
Proximity Label-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 NPTN 27020
Affinity Capture-MS Homo sapiens
61 GPR182  
Affinity Capture-MS Homo sapiens
62 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
63 RAB7A 7879
Proximity Label-MS Homo sapiens
64 HUWE1 10075
Affinity Capture-MS Homo sapiens
65 ARRDC3 57561
Affinity Capture-MS Homo sapiens
66 EVPL 2125
Affinity Capture-MS Homo sapiens
67 TGM1 7051
Affinity Capture-MS Homo sapiens
68 Coro1c 23790
Affinity Capture-MS Mus musculus
69 SERPINB7 8710
Affinity Capture-MS Homo sapiens
70 CACNG4  
Affinity Capture-MS Homo sapiens
71 TTC7B 145567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CALML3 810
Affinity Capture-MS Homo sapiens
73 MRPL44  
Two-hybrid Homo sapiens
74 APLNR  
Affinity Capture-MS Homo sapiens
75 DNAJC21  
Proximity Label-MS Homo sapiens
76 TYMP 1890
Affinity Capture-MS Homo sapiens
77 SORT1 6272
Affinity Capture-MS Homo sapiens
78 ARRDC4  
Affinity Capture-MS Homo sapiens
79 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 EPB41L4A 64097
Proximity Label-MS Homo sapiens
81 CD274 29126
Affinity Capture-MS Homo sapiens
82 CUL3 8452
Affinity Capture-MS Homo sapiens
83 NPY2R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 ARF6 382
Proximity Label-MS Homo sapiens
85 FOXL1  
Affinity Capture-MS Homo sapiens
86 METTL21B  
Affinity Capture-MS Homo sapiens
87 LOR  
Affinity Capture-MS Homo sapiens
88 SNX27 81609
Affinity Capture-MS Homo sapiens
89 SDC2 6383
Affinity Capture-MS Homo sapiens
90 FN1 2335
Affinity Capture-MS Homo sapiens
91 A2ML1 144568
Affinity Capture-MS Homo sapiens
92 CD226 10666
Affinity Capture-MS Homo sapiens
93 MRPL1  
Affinity Capture-MS Homo sapiens
94 CRYBB3  
Affinity Capture-MS Homo sapiens
95 FKBP5 2289
Affinity Capture-MS Homo sapiens
96 C12orf74  
Affinity Capture-MS Homo sapiens
97 PI4KAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 C3orf18  
Affinity Capture-MS Homo sapiens
99 CNR2  
Affinity Capture-MS Homo sapiens
100 SOHLH1  
Affinity Capture-MS Homo sapiens
101 GPR45  
Affinity Capture-MS Homo sapiens
102 RAB9A 9367
Proximity Label-MS Homo sapiens
103 CD244 51744
Affinity Capture-MS Homo sapiens
104 P2RY10  
Affinity Capture-MS Homo sapiens
105 KRAS 3845
Proximity Label-MS Homo sapiens
Negative Genetic Homo sapiens
106 HAX1  
Proximity Label-MS Homo sapiens
107 SDR9C7  
Affinity Capture-MS Homo sapiens
108 AKAP1 8165
Proximity Label-MS Homo sapiens
109 FLOT1 10211
Proximity Label-MS Homo sapiens
110 CCDC8  
Affinity Capture-MS Homo sapiens
111 TNNT1  
Two-hybrid Homo sapiens
112 DSC2 1824
Affinity Capture-MS Homo sapiens
113 C8orf33 65265
Two-hybrid Homo sapiens
114 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
115 COPZ1 22818
Affinity Capture-MS Homo sapiens
116 RNASE7 84659
Affinity Capture-MS Homo sapiens
117 LAMTOR1 55004
Proximity Label-MS Homo sapiens
118 SERPINA12  
Affinity Capture-MS Homo sapiens
119 JUP 3728
Affinity Capture-MS Homo sapiens
120 OPALIN  
Affinity Capture-MS Homo sapiens
121 MFSD4  
Affinity Capture-MS Homo sapiens
122 STRN4 29888
Two-hybrid Homo sapiens
123 EZR 7430
Proximity Label-MS Homo sapiens
124 LCK 3932
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
125 C17orf89  
Affinity Capture-MS Homo sapiens
126 GCLM 2730
Affinity Capture-MS Homo sapiens
127 KLK7 5650
Affinity Capture-MS Homo sapiens
128 CDSN 1041
Affinity Capture-MS Homo sapiens
129 S100A9 6280
Affinity Capture-MS Homo sapiens
130 C11orf52 91894
Proximity Label-MS Homo sapiens
131 MKI67  
Affinity Capture-MS Homo sapiens
132 NEFH 4744
Affinity Capture-MS Homo sapiens
133 ANAPC2 29882
Proximity Label-MS Homo sapiens
134 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 EVA1C  
Affinity Capture-MS Homo sapiens
136 RAB2A 5862
Proximity Label-MS Homo sapiens
137 SPN 6693
Affinity Capture-MS Homo sapiens
138 INF2 64423
Affinity Capture-MS Homo sapiens
139 C16orf72 29035
Affinity Capture-MS Homo sapiens
140 TTC7A 57217
Affinity Capture-MS Homo sapiens
141 PXN 5829
Proximity Label-MS Homo sapiens
142 RAB11A 8766
Proximity Label-MS Homo sapiens
143 SERPINB2 5055
Affinity Capture-MS Homo sapiens
144 NCCRP1 342897
Affinity Capture-MS Homo sapiens
145 EPHA2 1969
Proximity Label-MS Homo sapiens
146 TXNIP 10628
Affinity Capture-MS Homo sapiens
147 PTGES3 10728
Affinity Capture-MS Homo sapiens
148 Flnb 286940
Affinity Capture-MS Mus musculus
149 ECM1 1893
Affinity Capture-MS Homo sapiens
150 PI4KAP2  
Affinity Capture-MS Homo sapiens
151 HARS 3035
Co-fractionation Homo sapiens
152 CUL4A 8451
Affinity Capture-MS Homo sapiens
153 PPP3CC 5533
Affinity Capture-MS Homo sapiens
154 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here