Gene description for PLEKHA1
Gene name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Gene symbol PLEKHA1
Other names/aliases TAPP1
Species Homo sapiens
 Database cross references - PLEKHA1
ExoCarta ExoCarta_59338
Vesiclepedia VP_59338
Entrez Gene 59338
HGNC 14335
MIM 607772
UniProt Q9HB21  
 PLEKHA1 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for PLEKHA1
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    PDZ domain binding GO:0030165 IPI
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IBA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
Biological Process
    luteinization GO:0001553 IEA
    spermatogenesis GO:0007283 IEA
    androgen metabolic process GO:0008209 IEA
    estrogen metabolic process GO:0008210 IEA
    post-embryonic development GO:0009791 IEA
    ruffle organization GO:0031529 IDA
    Leydig cell differentiation GO:0033327 IEA
    multicellular organism growth GO:0035264 IEA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IDA
    establishment of protein localization GO:0045184 IDA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    skeletal system morphogenesis GO:0048705 IEA
    B cell receptor signaling pathway GO:0050853 IDA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IBA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IMP
    roof of mouth development GO:0060021 IEA
    face morphogenesis GO:0060325 IEA
    cellular response to hydrogen peroxide GO:0070301 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    ruffle membrane GO:0032587 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PLEKHA1 in exosomes
1
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PLEKHA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF43  
Proximity Label-MS Homo sapiens
2 LYN 4067
Proximity Label-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 FAM131B  
Affinity Capture-MS Homo sapiens
5 RAB35 11021
Proximity Label-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 PBX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 EBAG9 9166
Proximity Label-MS Homo sapiens
9 OCLN 100506658
Proximity Label-MS Homo sapiens
10 RAB7A 7879
Proximity Label-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 RAB9A 9367
Proximity Label-MS Homo sapiens
14 RAB5A 5868
Proximity Label-MS Homo sapiens
15 GJA1 2697
Proximity Label-MS Homo sapiens
16 SH3BP4 23677
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 DOCK7 85440
Affinity Capture-MS Homo sapiens
18 PARD3 56288
Proximity Label-MS Homo sapiens
19 EPB41L4A 64097
Proximity Label-MS Homo sapiens
20 LAMP1 3916
Proximity Label-MS Homo sapiens
21 WDR45B 56270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MSL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PTEN 5728
Proximity Label-MS Homo sapiens
24 ATG9A 79065
Proximity Label-MS Homo sapiens
25 LAMP2 3920
Proximity Label-MS Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 PLEKHA2 59339
Two-hybrid Homo sapiens
28 ARF6 382
Proximity Label-MS Homo sapiens
29 CXADR 1525
Proximity Label-MS Homo sapiens
30 STX4 6810
Proximity Label-MS Homo sapiens
31 C11orf52 91894
Proximity Label-MS Homo sapiens
32 ZNF610  
Affinity Capture-MS Homo sapiens
33 LATS1  
Proximity Label-MS Homo sapiens
34 RAB4A 5867
Proximity Label-MS Homo sapiens
35 DNAJC5 80331
Proximity Label-MS Homo sapiens
36 CDH1 999
Proximity Label-MS Homo sapiens
37 MLLT4 4301
Proximity Label-MS Homo sapiens
38 PTPN13 5783
Affinity Capture-MS Homo sapiens
39 DIRAS3  
Proximity Label-MS Homo sapiens
40 CRKL 1399
Two-hybrid Homo sapiens
41 LRRC61 65999
Affinity Capture-MS Homo sapiens
42 RAB11A 8766
Proximity Label-MS Homo sapiens
43 KRAS 3845
Proximity Label-MS Homo sapiens
44 RHOB 388
Proximity Label-MS Homo sapiens
45 LATS2 26524
Proximity Label-MS Homo sapiens
46 CAV1 857
Proximity Label-MS Homo sapiens
47 MPDZ  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
48 FLOT1 10211
Proximity Label-MS Homo sapiens
49 STX6 10228
Proximity Label-MS Homo sapiens
50 PPP1R15A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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