Gene description for VRK2
Gene name vaccinia related kinase 2
Gene symbol VRK2
Other names/aliases -
Species Homo sapiens
 Database cross references - VRK2
ExoCarta ExoCarta_7444
Vesiclepedia VP_7444
Entrez Gene 7444
HGNC 12719
MIM 602169
UniProt Q86Y07  
 VRK2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for VRK2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IDA
    protein domain specific binding GO:0019904 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    DNA damage response GO:0006974 IBA
    signal transduction GO:0007165 IBA
    cellular response to oxidative stress GO:0034599 IMP
    regulation of MAPK cascade GO:0043408 IMP
    protein autophosphorylation GO:0046777 IDA
    regulation of interleukin-1-mediated signaling pathway GO:2000659 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nuclear envelope GO:0005635 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    mitochondrial membrane GO:0031966 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified VRK2 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for VRK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 EIF4G3 8672
Affinity Capture-MS Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 OCLN 100506658
Proximity Label-MS Homo sapiens
5 CIB2  
Affinity Capture-MS Homo sapiens
6 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 LAMP3  
Proximity Label-MS Homo sapiens
9 ATG9A 79065
Proximity Label-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ARFGAP2 84364
Affinity Capture-MS Homo sapiens
13 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
14 CTDNEP1 23399
Proximity Label-MS Homo sapiens
15 RBX1 9978
Reconstituted Complex Homo sapiens
16 FPR1  
Affinity Capture-MS Homo sapiens
17 GJD3  
Proximity Label-MS Homo sapiens
18 LAMP2 3920
Proximity Label-MS Homo sapiens
19 DNAJC25 548645
Proximity Label-MS Homo sapiens
20 PTPN1 5770
Proximity Label-MS Homo sapiens
21 Ktn1  
Affinity Capture-MS Mus musculus
22 STX4 6810
Proximity Label-MS Homo sapiens
23 FFAR1  
Affinity Capture-MS Homo sapiens
24 CYP19A1  
Affinity Capture-MS Homo sapiens
25 FIS1 51024
Proximity Label-MS Homo sapiens
26 INSRR 3645
Affinity Capture-MS Homo sapiens
27 FAM57A  
Affinity Capture-MS Homo sapiens
28 PTCHD3  
Affinity Capture-MS Homo sapiens
29 RAB3B 5865
Proximity Label-MS Homo sapiens
30 NUP210 23225
Affinity Capture-MS Homo sapiens
31 SNRPC 6631
Affinity Capture-MS Homo sapiens
32 RCBTB2  
Affinity Capture-MS Homo sapiens
33 SSR1 6745
Proximity Label-MS Homo sapiens
34 MARCH5  
Proximity Label-MS Homo sapiens
35 PIGH  
Affinity Capture-MS Homo sapiens
36 C3orf52  
Affinity Capture-MS Homo sapiens
37 DNAJC16  
Proximity Label-MS Homo sapiens
38 TMEM192 201931
Affinity Capture-MS Homo sapiens
39 SLC25A46 91137
Proximity Label-MS Homo sapiens
40 Rpl35 66489
Affinity Capture-MS Mus musculus
41 STT3B 201595
Affinity Capture-MS Homo sapiens
42 IL17RA 23765
Affinity Capture-MS Homo sapiens
43 RAB35 11021
Proximity Label-MS Homo sapiens
44 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 EBAG9 9166
Proximity Label-MS Homo sapiens
46 MCAM 4162
Proximity Label-MS Homo sapiens
47 NTMT1 28989
Affinity Capture-MS Homo sapiens
48 MARCKS 4082
Proximity Label-MS Homo sapiens
49 MAPK8IP2  
Reconstituted Complex Homo sapiens
50 LMAN1 3998
Proximity Label-MS Homo sapiens
51 CCT2 10576
Two-hybrid Homo sapiens
52 NUP155 9631
Proximity Label-MS Homo sapiens
53 KIAA1715 80856
Proximity Label-MS Homo sapiens
54 PXMP2  
Proximity Label-MS Homo sapiens
55 RAB5A 5868
Proximity Label-MS Homo sapiens
56 DDOST 1650
Affinity Capture-MS Homo sapiens
57 PANX1 24145
Proximity Label-MS Homo sapiens
58 TCP1 6950
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
59 EVA1C  
Affinity Capture-MS Homo sapiens
60 C9orf91 203197
Affinity Capture-MS Homo sapiens
61 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
62 EMD 2010
Proximity Label-MS Homo sapiens
63 CXADR 1525
Proximity Label-MS Homo sapiens
64 CACNG7  
Affinity Capture-MS Homo sapiens
65 P2RY8  
Affinity Capture-MS Homo sapiens
66 SCAP  
Affinity Capture-MS Homo sapiens
67 LDHD  
Affinity Capture-MS Homo sapiens
68 CHRNB2  
Affinity Capture-MS Homo sapiens
69 RAB4A 5867
Proximity Label-MS Homo sapiens
70 MAPK8IP1 9479
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 DTNBP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
72 OGT 8473
Reconstituted Complex Homo sapiens
73 RPL31 6160
Proximity Label-MS Homo sapiens
74 PCDHB3  
Affinity Capture-MS Homo sapiens
75 CCDC107  
Affinity Capture-MS Homo sapiens
76 LAMP1 3916
Proximity Label-MS Homo sapiens
77 RPN1 6184
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 MAPK8 5599
Affinity Capture-Western Homo sapiens
79 DHX38 9785
Affinity Capture-MS Homo sapiens
80 RHOT2 89941
Proximity Label-MS Homo sapiens
81 ELOVL5 60481
Proximity Label-MS Homo sapiens
82 SEC61B 10952
Proximity Label-MS Homo sapiens
83 RMDN3 55177
Proximity Label-MS Homo sapiens
84 MAP2K7 5609
Affinity Capture-Western Homo sapiens
85 REEP5 7905
Proximity Label-MS Homo sapiens
86 CYP26B1  
Affinity Capture-MS Homo sapiens
87 SEC63 11231
Proximity Label-MS Homo sapiens
88 MGARP  
Affinity Capture-MS Homo sapiens
89 TMED9 54732
Affinity Capture-MS Homo sapiens
90 RPN2 6185
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CANX 821
Affinity Capture-MS Homo sapiens
92 LRRC59 55379
Proximity Label-MS Homo sapiens
93 LMNB1 4001
Proximity Label-MS Homo sapiens
94 KCNC3 3748
Affinity Capture-MS Homo sapiens
95 DUOXA2  
Affinity Capture-MS Homo sapiens
96 TMPRSS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 PTH2R  
Affinity Capture-MS Homo sapiens
98 TMEM9B 56674
Affinity Capture-MS Homo sapiens
99 GPR182  
Affinity Capture-MS Homo sapiens
100 BCAP31 10134
Proximity Label-MS Homo sapiens
101 HSD3B7 80270
Proximity Label-MS Homo sapiens
102 STIM1 6786
Proximity Label-MS Homo sapiens
103 CCT4 10575
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
104 FKBP8 23770
Proximity Label-MS Homo sapiens
105 ARF6 382
Proximity Label-MS Homo sapiens
106 PLK1 5347
Biochemical Activity Homo sapiens
107 FAM187B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 CDC42 998
Two-hybrid Homo sapiens
109 SF3A1 10291
Affinity Capture-MS Homo sapiens
110 CYP2C18  
Affinity Capture-MS Homo sapiens
111 PRKACA 5566
Proximity Label-MS Homo sapiens
112 ATP2A1 487
Proximity Label-MS Homo sapiens
113 GPR45  
Affinity Capture-MS Homo sapiens
114 RAB9A 9367
Proximity Label-MS Homo sapiens
115 EVA1B 55194
Affinity Capture-MS Homo sapiens
116 DHFRL1  
Proximity Label-MS Homo sapiens
117 DDRGK1 65992
Affinity Capture-MS Homo sapiens
118 ILK 3611
Affinity Capture-MS Homo sapiens
119 CYP2C9  
Proximity Label-MS Homo sapiens
120 MAP3K7  
Affinity Capture-Western Homo sapiens
121 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
122 ACBD5 91452
Proximity Label-MS Homo sapiens
123 OST4  
Affinity Capture-MS Homo sapiens
124 PRADC1  
Affinity Capture-MS Homo sapiens
125 FTL 2512
Affinity Capture-MS Homo sapiens
126 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
127 KCNA2  
Affinity Capture-MS Homo sapiens
128 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
129 TP53 7157
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
130 MFSD4  
Affinity Capture-MS Homo sapiens
131 Nfatc2  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
132 TSPAN17 26262
Affinity Capture-MS Homo sapiens
133 COX8A  
Proximity Label-MS Homo sapiens
134 NUP35 129401
Proximity Label-MS Homo sapiens
135 ERGIC1 57222
Proximity Label-MS Homo sapiens
136 KCNE3  
Affinity Capture-MS Homo sapiens
137 MKI67  
Affinity Capture-MS Homo sapiens
138 DNAJC14  
Proximity Label-MS Homo sapiens
139 ERGIC2 51290
Proximity Label-MS Homo sapiens
140 ASB14  
Affinity Capture-MS Homo sapiens
141 SEC62 7095
Proximity Label-MS Homo sapiens
142 B3GAT1  
Proximity Label-MS Homo sapiens
143 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 RAB7A 7879
Proximity Label-MS Homo sapiens
145 RAB11A 8766
Proximity Label-MS Homo sapiens
146 CIT 11113
Affinity Capture-MS Homo sapiens
147 MARCH4  
Affinity Capture-MS Homo sapiens
148 DNAJC1 64215
Proximity Label-MS Homo sapiens
149 KRAS 3845
Proximity Label-MS Homo sapiens
150 RAB5C 5878
Proximity Label-MS Homo sapiens
151 C9orf72  
Affinity Capture-MS Homo sapiens
152 CCDC47 57003
Affinity Capture-MS Homo sapiens
153 CKAP4 10970
Proximity Label-MS Homo sapiens
154 SLC1A1 6505
Affinity Capture-MS Homo sapiens
155 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 LNX1  
Two-hybrid Homo sapiens
157 DERL1 79139
Proximity Label-MS Homo sapiens
158 DNAJC22  
Proximity Label-MS Homo sapiens
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