Gene description for SMPD4
Gene name sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3)
Gene symbol SMPD4
Other names/aliases NET13
NSMASE-3
NSMASE3
Species Homo sapiens
 Database cross references - SMPD4
ExoCarta ExoCarta_55627
Entrez Gene 55627
HGNC 32949
MIM 610457
UniProt Q9NXE4  
 SMPD4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SMPD4
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 TAS
    metal ion binding GO:0046872 IEA
    sphingomyelin phosphodiesterase D activity GO:0050290 IDA
Biological Process
    sphingomyelin catabolic process GO:0006685 IDA
    ceramide biosynthetic process GO:0046513 IDA
    glycosphingolipid metabolic process GO:0006687 TAS
    sphingolipid metabolic process GO:0006665 TAS
    glycerophospholipid catabolic process GO:0046475 IDA
    cellular response to tumor necrosis factor GO:0071356 IDA
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    trans-Golgi network GO:0005802 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified SMPD4 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SMPD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRF2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SMPD4 is involved
PathwayEvidenceSource
Glycosphingolipid metabolism TAS Reactome





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