Gene description for DNAJB11
Gene name DnaJ (Hsp40) homolog, subfamily B, member 11
Gene symbol DNAJB11
Other names/aliases ABBP-2
ABBP2
DJ9
Dj-9
EDJ
ERdj3
ERj3
ERj3p
PRO1080
UNQ537
Species Homo sapiens
 Database cross references - DNAJB11
ExoCarta ExoCarta_51726
Vesiclepedia VP_51726
Entrez Gene 51726
HGNC 14889
MIM 611341
UniProt Q9UBS4  
 DNAJB11 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for DNAJB11
Molecular Function
    signaling receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    unfolded protein binding GO:0051082 IBA
    misfolded protein binding GO:0051787 IBA
    misfolded protein binding GO:0051787 IDA
Biological Process
    protein folding GO:0006457 IEA
    mRNA modification GO:0016556 IEA
    positive regulation of ATP-dependent activity GO:0032781 IDA
    negative regulation of neurogenesis GO:0050768 IEA
    protein maturation GO:0051604 IBA
    protein maturation GO:0051604 IMP
Subcellular Localization
    extracellular space GO:0005615 IEA
    nucleus GO:0005634 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    membrane GO:0016020 HDA
    endoplasmic reticulum chaperone complex GO:0034663 IEA
    protein folding chaperone complex GO:0101031 IPI
 Experiment description of studies that identified DNAJB11 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
10
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
13
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
14
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for DNAJB11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 COL6A1 1291
Two-hybrid Homo sapiens
3 UGGT2 55757
Proximity Label-MS Homo sapiens
4 P4HB 5034
Proximity Label-MS Homo sapiens
5 MCM7 4176
Affinity Capture-MS Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 PAXIP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 POT1  
Affinity Capture-MS Homo sapiens
9 ARHGAP39  
Affinity Capture-MS Homo sapiens
10 RIN3  
Affinity Capture-MS Homo sapiens
11 NUCB2 4925
Proximity Label-MS Homo sapiens
12 SSB 6741
Co-fractionation Homo sapiens
13 ADRB2  
Affinity Capture-MS Homo sapiens
14 ERAP2 64167
Proximity Label-MS Homo sapiens
15 COL14A1 7373
Proximity Label-MS Homo sapiens
16 TUBB8 347688
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSPA1L 3305
Affinity Capture-MS Homo sapiens
18 NUBPL 80224
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ZZZ3  
Two-hybrid Homo sapiens
20 SEC63 11231
Proximity Label-MS Homo sapiens
21 ROCK2 9475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
22 GRAMD1A  
Proximity Label-MS Homo sapiens
23 ANKZF1 55139
Two-hybrid Homo sapiens
24 PTPN1 5770
Co-fractionation Homo sapiens
25 PARK7 11315
Proximity Label-MS Homo sapiens
26 ASXL1  
Affinity Capture-MS Homo sapiens
27 TMX1 81542
Proximity Label-MS Homo sapiens
28 RNF40 9810
Co-fractionation Homo sapiens
29 KIAA1429 25962
Affinity Capture-MS Homo sapiens
30 CUL7 9820
Affinity Capture-MS Homo sapiens
31 CAPZB 832
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
32 PLOD3 8985
Proximity Label-MS Homo sapiens
33 B3GNT2 10678
Affinity Capture-MS Homo sapiens
34 PRDX3 10935
Co-fractionation Homo sapiens
35 CEP70  
Two-hybrid Homo sapiens
36 DYRK1A 1859
Affinity Capture-MS Homo sapiens
37 PDIA5 10954
Proximity Label-MS Homo sapiens
38 MAN2B1 4125
Co-fractionation Homo sapiens
39 KIF5B 3799
Co-fractionation Homo sapiens
40 SEC24C 9632
Proximity Label-MS Homo sapiens
41 TMEM231 79583
Affinity Capture-MS Homo sapiens
42 BORA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 IMPDH2 3615
Co-fractionation Homo sapiens
44 NUP210 23225
Proximity Label-MS Homo sapiens
45 KCTD21 283219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
47 ATP5O 539
Proximity Label-MS Homo sapiens
48 SMOC1 64093
Co-fractionation Homo sapiens
49 UGGT1 56886
Proximity Label-MS Homo sapiens
50 FAF1 11124
Two-hybrid Homo sapiens
51 CRMP1 1400
Two-hybrid Homo sapiens
52 DNAJC8 22826
Co-fractionation Homo sapiens
53 NDUFV1 4723
Co-fractionation Homo sapiens
54 ATG16L1 55054
Affinity Capture-MS Homo sapiens
55 PRDX4 10549
Proximity Label-MS Homo sapiens
56 HSPA6 3310
Affinity Capture-MS Homo sapiens
57 GTSE1 51512
Affinity Capture-MS Homo sapiens
58 CALR 811
Proximity Label-MS Homo sapiens
59 METTL23  
Affinity Capture-MS Homo sapiens
60 MYCN  
Affinity Capture-MS Homo sapiens
61 UBQLN4 56893
Two-hybrid Homo sapiens
62 DNAJB12 54788
Affinity Capture-MS Homo sapiens
63 FKBP10 60681
Proximity Label-MS Homo sapiens
64 FAM20C 56975
Affinity Capture-MS Homo sapiens
65 PSMC3 5702
Co-fractionation Homo sapiens
66 KIF14 9928
Affinity Capture-MS Homo sapiens
67 TMTC3 160418
Proximity Label-MS Homo sapiens
68 HSPA8 3312
Co-fractionation Homo sapiens
69 FAS 355
Proximity Label-MS Homo sapiens
70 LMAN1 3998
Proximity Label-MS Homo sapiens
71 BAG5 9529
Affinity Capture-MS Homo sapiens
72 LRRC59 55379
Proximity Label-MS Homo sapiens
73 DDOST 1650
Proximity Label-MS Homo sapiens
74 CLPTM1L 81037
Proximity Label-MS Homo sapiens
75 LAMC1 3915
Proximity Label-MS Homo sapiens
76 P4HA1 5033
Proximity Label-MS Homo sapiens
77 COASY 80347
Proximity Label-MS Homo sapiens
78 ERO1L 30001
Proximity Label-MS Homo sapiens
79 SDF2 6388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 ADI1 55256
Proximity Label-MS Homo sapiens
81 EMD 2010
Affinity Capture-MS Homo sapiens
82 CD74 972
Affinity Capture-MS Homo sapiens
83 ERBB3 2065
Affinity Capture-MS Homo sapiens
84 ADA 100
Proximity Label-MS Homo sapiens
85 HSP90B1 7184
Proximity Label-MS Homo sapiens
86 SLC25A30 253512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 SIMC1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 TUBA1A 7846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 PLOD2 5352
Proximity Label-MS Homo sapiens
90 BAG6 7917
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
91 ACP2 53
Co-fractionation Homo sapiens
92 Vav2  
Affinity Capture-MS Mus musculus
93 PTPN21 11099
Affinity Capture-MS Homo sapiens
94 PLD3 23646
Proximity Label-MS Homo sapiens
95 CLCC1 23155
Proximity Label-MS Homo sapiens
96 SDF2L1 23753
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 VCP 7415
Affinity Capture-MS Homo sapiens
98 DDX5 1655
Co-fractionation Homo sapiens
99 NTRK1 4914
Affinity Capture-MS Homo sapiens
100 IFI16 3428
Affinity Capture-MS Homo sapiens
101 ANXA4 307
Co-fractionation Homo sapiens
102 SCCPDH 51097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 ERP29 10961
Proximity Label-MS Homo sapiens
104 MIA3 375056
Proximity Label-MS Homo sapiens
105 MOGS 7841
Proximity Label-MS Homo sapiens
106 CDHR4  
Affinity Capture-MS Homo sapiens
107 TRIM11  
Affinity Capture-MS Homo sapiens
108 RPN1 6184
Proximity Label-MS Homo sapiens
109 PCSK9 255738
Affinity Capture-MS Homo sapiens
110 PNP 4860
Co-fractionation Homo sapiens
111 PDIA6 10130
Proximity Label-MS Homo sapiens
112 DNAJC30  
Proximity Label-MS Homo sapiens
113 DRAP1 10589
Two-hybrid Homo sapiens
114 PTPRO 5800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 SEC61B 10952
Proximity Label-MS Homo sapiens
116 CYLD  
Affinity Capture-MS Homo sapiens
117 KDF1  
Affinity Capture-MS Homo sapiens
118 DDAH2 23564
Two-hybrid Homo sapiens
119 SNX1 6642
Affinity Capture-MS Homo sapiens
120 RRM1 6240
Co-fractionation Homo sapiens
121 PTN  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
122 ECH1 1891
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
123 DOK2 9046
Affinity Capture-MS Homo sapiens
124 HSPA5 3309
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 NOMO2 283820
Proximity Label-MS Homo sapiens
126 EFNA4  
Affinity Capture-MS Homo sapiens
127 COL4A2 1284
Proximity Label-MS Homo sapiens
128 IPO5 3843
Co-fractionation Homo sapiens
129 AIFM1 9131
Co-fractionation Homo sapiens
130 CANX 821
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
131 TMPO 7112
Affinity Capture-MS Homo sapiens
132 ALS2  
Affinity Capture-MS Homo sapiens
133 CALR3  
Proximity Label-MS Homo sapiens
134 SDHA 6389
Affinity Capture-MS Homo sapiens
135 BAG2 9532
Affinity Capture-MS Homo sapiens
136 RNF128  
Affinity Capture-MS Homo sapiens
137 ATP5H 10476
Proximity Label-MS Homo sapiens
138 PRKAR2A 5576
Co-fractionation Homo sapiens
139 CDC42EP1 11135
Affinity Capture-MS Homo sapiens
140 BCAP31 10134
Proximity Label-MS Homo sapiens
141 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 NR3C1 2908
Proximity Label-MS Homo sapiens
143 MECP2 4204
Affinity Capture-MS Homo sapiens
144 PDIA3 2923
Proximity Label-MS Homo sapiens
145 EGFR 1956
Affinity Capture-MS Homo sapiens
146 FBXO6 26270
Affinity Capture-MS Homo sapiens
147 RIPK4  
Affinity Capture-MS Homo sapiens
148 RCN1 5954
Proximity Label-MS Homo sapiens
149 TCP10L2  
Affinity Capture-MS Homo sapiens
150 TUBA4A 7277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 PRKCSH 5589
Proximity Label-MS Homo sapiens
152 AGO4  
Affinity Capture-MS Homo sapiens
153 Sept2  
Affinity Capture-MS Mus musculus
154 UBR1 197131
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
155 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 EMC2 9694
Proximity Label-MS Homo sapiens
157 GJB1 2705
Affinity Capture-MS Homo sapiens
158 PTPRN  
Proximity Label-MS Homo sapiens
159 DNAJC10 54431
Proximity Label-MS Homo sapiens
160 DNAJC3 5611
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
161 HYOU1 10525
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
162 ILK 3611
Affinity Capture-MS Homo sapiens
163 GANAB 23193
Proximity Label-MS Homo sapiens
164 APOBEC1  
Reconstituted Complex Homo sapiens
165 HSPD1 3329
Co-fractionation Homo sapiens
166 CORO1C 23603
Co-fractionation Homo sapiens
167 AKAP1 8165
Proximity Label-MS Homo sapiens
168 UBXN1 51035
Co-fractionation Homo sapiens
169 SNX5 27131
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
170 NADK2 133686
Co-fractionation Homo sapiens
171 HUWE1 10075
Affinity Capture-MS Homo sapiens
172 EIF3D 8664
Two-hybrid Homo sapiens
173 DNAJC13 23317
Two-hybrid Homo sapiens
174 GSR 2936
Proximity Label-MS Homo sapiens
175 STUB1 10273
Affinity Capture-MS Homo sapiens
176 PELO 53918
Affinity Capture-MS Homo sapiens
177 TTC13  
Proximity Label-MS Homo sapiens
178 IKBKAP 8518
Affinity Capture-MS Homo sapiens
179 PRMT3 10196
Co-fractionation Homo sapiens
180 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
181 FASN 2194
Co-fractionation Homo sapiens
182 TMED10 10972
Proximity Label-MS Homo sapiens
183 ACP1 52
Proximity Label-MS Homo sapiens
184 TERF2  
Affinity Capture-MS Homo sapiens
185 ITSN1 6453
Affinity Capture-MS Homo sapiens
186 GRB2 2885
Two-hybrid Homo sapiens
187 CKAP4 10970
Proximity Label-MS Homo sapiens
188 IKBIP 121457
Proximity Label-MS Homo sapiens
189 AGO1  
Affinity Capture-MS Homo sapiens
190 SEMA6B 10501
Affinity Capture-MS Homo sapiens
191 PDIA4 9601
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
192 CALU 813
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
193 EMC1 23065
Proximity Label-MS Homo sapiens
194 EMC7 56851
Proximity Label-MS Homo sapiens
195 TXNDC5 81567
Proximity Label-MS Homo sapiens
196 TUBG1 7283
Affinity Capture-MS Homo sapiens
197 EFNA3  
Affinity Capture-MS Homo sapiens
198 EPHX1 2052
Co-fractionation Homo sapiens
199 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
200 PLOD1 5351
Proximity Label-MS Homo sapiens
201 WTAP 9589
Affinity Capture-MS Homo sapiens
202 P3H1 64175
Proximity Label-MS Homo sapiens
203 TOR1AIP1 26092
Proximity Label-MS Homo sapiens
204 OS9 10956
Affinity Capture-MS Homo sapiens
205 HDAC5 10014
Affinity Capture-MS Homo sapiens
206 EIF4H 7458
Affinity Capture-MS Homo sapiens
207 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
208 GSTM3 2947
Proximity Label-MS Homo sapiens
209 LONP1 9361
Co-fractionation Homo sapiens
210 LRPAP1 4043
Proximity Label-MS Homo sapiens
211 EZH2  
Two-hybrid Homo sapiens
212 PARS2 25973
Affinity Capture-MS Homo sapiens
213 ZNRF4  
Affinity Capture-MS Homo sapiens
214 FLNA 2316
Co-fractionation Homo sapiens
215 CBWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
216 BRD1 23774
Affinity Capture-MS Homo sapiens
217 MAGT1 84061
Proximity Label-MS Homo sapiens
218 STT3B 201595
Proximity Label-MS Homo sapiens
219 SNX2 6643
Affinity Capture-MS Homo sapiens
220 DNASE2 1777
Co-fractionation Homo sapiens
221 COLGALT1 79709
Proximity Label-MS Homo sapiens
222 C9orf72  
Affinity Capture-MS Homo sapiens
223 GPX7 2882
Affinity Capture-MS Homo sapiens
224 SPRED1 161742
Two-hybrid Homo sapiens
225 Sept9  
Affinity Capture-MS Mus musculus
226 TTN 7273
Cross-Linking-MS (XL-MS) Homo sapiens
227 PPIB 5479
Proximity Label-MS Homo sapiens
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