Gene description for PREB
Gene name prolactin regulatory element binding
Gene symbol PREB
Other names/aliases SEC12
Species Homo sapiens
 Database cross references - PREB
ExoCarta ExoCarta_10113
Vesiclepedia VP_10113
Entrez Gene 10113
HGNC 9356
MIM 606395
UniProt Q9HCU5  
 PREB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PREB
Molecular Function
    DNA binding GO:0003677 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
    GTPase binding GO:0051020 IEA
Biological Process
    regulation of COPII vesicle coating GO:0003400 IBA
    regulation of COPII vesicle coating GO:0003400 IDA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISS
    lipoprotein transport GO:0042953 ISS
    COPII vesicle coating GO:0048208 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    endoplasmic reticulum exit site GO:0070971 IDA
 Experiment description of studies that identified PREB in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PREB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAMP3  
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 ICOSLG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 OCLN 100506658
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 SRPRB 58477
Affinity Capture-MS Homo sapiens
7 RIT1 6016
Negative Genetic Homo sapiens
8 GJD3  
Proximity Label-MS Homo sapiens
9 DNAJC25 548645
Proximity Label-MS Homo sapiens
10 PTPN1 5770
Proximity Label-MS Homo sapiens
11 STX4 6810
Proximity Label-MS Homo sapiens
12 TMEM231 79583
Affinity Capture-MS Homo sapiens
13 TEX264 51368
Affinity Capture-MS Homo sapiens
14 B3GAT1  
Proximity Label-MS Homo sapiens
15 OGDH 4967
Co-fractionation Homo sapiens
16 CYB5R1 51706
Co-fractionation Homo sapiens
17 CXADR 1525
Proximity Label-MS Homo sapiens
18 CYP19A1  
Affinity Capture-MS Homo sapiens
19 RAB3B 5865
Proximity Label-MS Homo sapiens
20 GPRC5B 51704
Affinity Capture-MS Homo sapiens
21 SSR1 6745
Proximity Label-MS Homo sapiens
22 PIGH  
Affinity Capture-MS Homo sapiens
23 KRAS 3845
Negative Genetic Homo sapiens
24 C3orf52  
Affinity Capture-MS Homo sapiens
25 SLC25A46 91137
Proximity Label-MS Homo sapiens
26 RAB35 11021
Proximity Label-MS Homo sapiens
27 CTAGE5 4253
Affinity Capture-MS Homo sapiens
28 FAM20C 56975
Affinity Capture-MS Homo sapiens
29 MARCKS 4082
Proximity Label-MS Homo sapiens
30 LMAN1 3998
Proximity Label-MS Homo sapiens
31 KIAA1715 80856
Proximity Label-MS Homo sapiens
32 PXMP2  
Proximity Label-MS Homo sapiens
33 NF2 4771
Affinity Capture-MS Homo sapiens
34 EGLN3  
Affinity Capture-MS Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
36 EMD 2010
Proximity Label-MS Homo sapiens
37 ACOT9 23597
Co-fractionation Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 AMFR 267
Affinity Capture-MS Homo sapiens
40 MSX2  
Affinity Capture-MS Homo sapiens
41 SCAP  
Affinity Capture-MS Homo sapiens
42 ANO4 121601
Affinity Capture-MS Homo sapiens
43 RAB4A 5867
Proximity Label-MS Homo sapiens
44 SLC25A20 788
Co-fractionation Homo sapiens
45 PCDHB3  
Affinity Capture-MS Homo sapiens
46 RPN1 6184
Proximity Label-MS Homo sapiens
47 Preb  
Affinity Capture-MS Mus musculus
48 AGPS 8540
Co-fractionation Homo sapiens
49 RHOT2 89941
Proximity Label-MS Homo sapiens
50 ELOVL5 60481
Proximity Label-MS Homo sapiens
51 VDAC2 7417
Co-fractionation Homo sapiens
52 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RMDN3 55177
Proximity Label-MS Homo sapiens
54 REEP5 7905
Proximity Label-MS Homo sapiens
55 SEC63 11231
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
56 ANO6 196527
Affinity Capture-MS Homo sapiens
57 HADHA 3030
Co-fractionation Homo sapiens
58 RPN2 6185
Proximity Label-MS Homo sapiens
59 CANX 821
Affinity Capture-MS Homo sapiens
60 LRRC59 55379
Proximity Label-MS Homo sapiens
61 PANX1 24145
Proximity Label-MS Homo sapiens
62 ZDHHC12  
Affinity Capture-MS Homo sapiens
63 LETM1 3954
Co-fractionation Homo sapiens
64 CHRNA1  
Affinity Capture-MS Homo sapiens
65 BCAP31 10134
Proximity Label-MS Homo sapiens
66 HSD3B7 80270
Proximity Label-MS Homo sapiens
67 CYB5R3 1727
Co-fractionation Homo sapiens
68 STIM1 6786
Proximity Label-MS Homo sapiens
69 FKBP8 23770
Proximity Label-MS Homo sapiens
70 CORO1C 23603
Co-fractionation Homo sapiens
71 CNR2  
Affinity Capture-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 ATP2A1 487
Proximity Label-MS Homo sapiens
74 LMNB1 4001
Proximity Label-MS Homo sapiens
75 RAB9A 9367
Proximity Label-MS Homo sapiens
76 DHFRL1  
Proximity Label-MS Homo sapiens
77 DDRGK1 65992
Affinity Capture-MS Homo sapiens
78 ENTPD2 954
Affinity Capture-MS Homo sapiens
79 CYP2C9  
Proximity Label-MS Homo sapiens
80 COX2 4513
Co-fractionation Homo sapiens
81 CDC7  
Affinity Capture-MS Homo sapiens
82 KCNK16  
Affinity Capture-MS Homo sapiens
83 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
84 TMED10 10972
Affinity Capture-MS Homo sapiens
85 NAAA  
Affinity Capture-MS Homo sapiens
86 PARP1 142
Proximity Label-MS Homo sapiens
87 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
88 SIDT2 51092
Affinity Capture-MS Homo sapiens
89 LDLRAD1  
Affinity Capture-MS Homo sapiens
90 WWOX 51741
Affinity Capture-MS Homo sapiens
91 NHLRC2 374354
Affinity Capture-MS Homo sapiens
92 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
93 NUP35 129401
Proximity Label-MS Homo sapiens
94 GNG5 2787
Co-fractionation Homo sapiens
95 SEC62 7095
Proximity Label-MS Homo sapiens
96 RAB7A 7879
Proximity Label-MS Homo sapiens
97 LPAR2 9170
Affinity Capture-MS Homo sapiens
98 MARCH4  
Affinity Capture-MS Homo sapiens
99 DNAJC1 64215
Proximity Label-MS Homo sapiens
100 ATP5C1 509
Co-fractionation Homo sapiens
101 RAB5C 5878
Proximity Label-MS Homo sapiens
102 C9orf72  
Affinity Capture-MS Homo sapiens
103 CCDC47 57003
Affinity Capture-MS Homo sapiens
104 CKAP4 10970
Proximity Label-MS Homo sapiens
105 DERL1 79139
Proximity Label-MS Homo sapiens
106 EP300 2033
Affinity Capture-MS Homo sapiens
107 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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