Gene description for BRD3
Gene name bromodomain containing 3
Gene symbol BRD3
Other names/aliases ORFX
RING3L
Species Homo sapiens
 Database cross references - BRD3
ExoCarta ExoCarta_8019
Vesiclepedia VP_8019
Entrez Gene 8019
HGNC 1104
MIM 601541
UniProt Q15059  
 BRD3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for BRD3
Molecular Function
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IBA
    lysine-acetylated histone binding GO:0070577 IDA
    lncRNA binding GO:0106222 IDA
    molecular condensate scaffold activity GO:0140693 IDA
Biological Process
    chromatin remodeling GO:0006338 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    endodermal cell differentiation GO:0035987 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    protein localization to chromatin GO:0071168 IDA
Subcellular Localization
    chromatin GO:0000785 IBA
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified BRD3 in exosomes
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for BRD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC14B 8555
Proximity Label-MS Homo sapiens
2 HIST1H4K 8362
Affinity Capture-MS Homo sapiens
3 SRPK2 6733
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
4 Nfyc 18046
Affinity Capture-MS Mus musculus
5 JPH1 56704
Affinity Capture-MS Homo sapiens
6 SMARCE1 6605
Affinity Capture-MS Homo sapiens
7 BRD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
9 PRPF40A 55660
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TAF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 AKAP8L  
Affinity Capture-MS Homo sapiens
13 CENPA  
Proximity Label-MS Homo sapiens
14 SATB2  
Affinity Capture-MS Homo sapiens
15 APEX1 328
Affinity Capture-RNA Homo sapiens
Proximity Label-MS Homo sapiens
16 MAP1LC3B 81631
Two-hybrid Homo sapiens
17 MAP3K5 4217
Affinity Capture-MS Homo sapiens
18 WDR76  
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
19 GATAD2B 57459
Affinity Capture-MS Homo sapiens
20 WHSC1 7468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PRPF40B  
Affinity Capture-MS Homo sapiens
22 CHAF1A  
Affinity Capture-MS Homo sapiens
23 CTDSPL2  
Affinity Capture-MS Homo sapiens
24 NFKBIL1  
Affinity Capture-MS Homo sapiens
25 CDK2AP1  
Affinity Capture-MS Homo sapiens
26 PLCH1  
Affinity Capture-MS Homo sapiens
27 HMGN2 3151
Cross-Linking-MS (XL-MS) Homo sapiens
28 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
29 ATAD5  
Affinity Capture-MS Homo sapiens
30 HELLS 3070
Affinity Capture-MS Homo sapiens
31 AMD1  
Affinity Capture-MS Homo sapiens
32 PSMG1 8624
Affinity Capture-MS Homo sapiens
33 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 BCS1L 617
Affinity Capture-MS Homo sapiens
35 ANAPC16  
Affinity Capture-MS Homo sapiens
36 SREK1 140890
Affinity Capture-MS Homo sapiens
37 KIF14 9928
Affinity Capture-MS Homo sapiens
38 SUV420H1  
Affinity Capture-MS Homo sapiens
39 UNK  
Affinity Capture-RNA Homo sapiens
40 SPDYE7P  
Affinity Capture-MS Homo sapiens
41 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 UTP14A 10813
Affinity Capture-MS Homo sapiens
43 TXNL4B  
Affinity Capture-MS Homo sapiens
44 RFC5 5985
Affinity Capture-MS Homo sapiens
45 TOP2B 7155
Affinity Capture-MS Homo sapiens
46 MEPCE 56257
Affinity Capture-MS Homo sapiens
47 PGAM5 192111
Affinity Capture-MS Homo sapiens
48 C17orf49 124944
Affinity Capture-MS Homo sapiens
49 PLD1 5337
Affinity Capture-MS Homo sapiens
50 TIGD5  
Affinity Capture-MS Homo sapiens
51 RPL31 6160
Proximity Label-MS Homo sapiens
52 CBX5 23468
Affinity Capture-MS Homo sapiens
53 GTF3C2  
Affinity Capture-MS Homo sapiens
54 GTF2H2C_2  
Affinity Capture-MS Homo sapiens
55 BANF1 8815
Affinity Capture-MS Homo sapiens
56 ZFP91 80829
Affinity Capture-MS Homo sapiens
57 RFC4 5984
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 WHSC1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RRP1B 23076
Affinity Capture-MS Homo sapiens
60 SON 6651
Affinity Capture-MS Homo sapiens
61 GTPBP4 23560
Affinity Capture-MS Homo sapiens
62 MED15  
Affinity Capture-MS Homo sapiens
63 CD3EAP  
Proximity Label-MS Homo sapiens
64 IMMT 10989
Cross-Linking-MS (XL-MS) Homo sapiens
65 TMPO 7112
Affinity Capture-MS Homo sapiens
66 GTF3C3 9330
Affinity Capture-MS Homo sapiens
67 NTRK1 4914
Affinity Capture-MS Homo sapiens
68 C1orf226  
Affinity Capture-MS Homo sapiens
69 TBC1D10B 26000
Affinity Capture-MS Homo sapiens
70 RAD18  
Affinity Capture-MS Homo sapiens
71 FAHD1 81889
Affinity Capture-MS Homo sapiens
72 RPL7L1 285855
Affinity Capture-MS Homo sapiens
73 SS18  
Affinity Capture-MS Homo sapiens
74 NTHL1  
Affinity Capture-MS Homo sapiens
75 HIST1H4B 8366
Affinity Capture-MS Homo sapiens
76 CCNL2 81669
Affinity Capture-MS Homo sapiens
77 MFAP1  
Affinity Capture-MS Homo sapiens
78 NUCB1 4924
Affinity Capture-MS Homo sapiens
79 ZMYND8 23613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 RPL35A 6165
Affinity Capture-MS Homo sapiens
81 MYO9B 4650
Affinity Capture-MS Homo sapiens
82 ZNF687  
Affinity Capture-MS Homo sapiens
83 EIF3CL 728689
Affinity Capture-MS Homo sapiens
84 MED23 9439
Affinity Capture-MS Homo sapiens
85 SMARCD2 6603
Affinity Capture-MS Homo sapiens
86 HIST1H4I 8294
Affinity Capture-MS Homo sapiens
87 SLC25A6 293
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
88 HEATR1 55127
Affinity Capture-MS Homo sapiens
89 SUPT16H 11198
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
90 NUDT21 11051
Affinity Capture-MS Homo sapiens
91 BCAS2 10286
Affinity Capture-MS Homo sapiens
92 CHTOP  
Affinity Capture-MS Homo sapiens
93 RRS1 23212
Affinity Capture-MS Homo sapiens
94 RBM25 58517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RUVBL2 10856
Co-fractionation Homo sapiens
96 TCERG1 10915
Affinity Capture-MS Homo sapiens
97 KDM1B  
Affinity Capture-MS Homo sapiens
98 SRSF9 8683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 KAT7  
Affinity Capture-MS Homo sapiens
100 MTA1 9112
Affinity Capture-MS Homo sapiens
101 TBL3 10607
Affinity Capture-MS Homo sapiens
102 TRA2B 6434
Affinity Capture-MS Homo sapiens
103 RAB11FIP1 80223
Affinity Capture-MS Homo sapiens
104 PWWP2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 ERCC3  
Affinity Capture-MS Homo sapiens
106 LARP7 51574
Affinity Capture-MS Homo sapiens
107 HIST1H4L 8368
Affinity Capture-MS Homo sapiens
108 ZNF711  
Affinity Capture-MS Homo sapiens
109 HIST2H2AA4 723790
Affinity Capture-MS Homo sapiens
110 HIST1H4J 8363
Affinity Capture-MS Homo sapiens
111 POLR3B 55703
Affinity Capture-MS Homo sapiens
112 ZNF740  
Affinity Capture-MS Homo sapiens
113 EHMT1  
Affinity Capture-MS Homo sapiens
114 ISLR2  
Affinity Capture-MS Homo sapiens
115 VANGL1 81839
Affinity Capture-MS Homo sapiens
116 DNMT1 1786
Affinity Capture-MS Homo sapiens
117 FAM92A1  
Affinity Capture-MS Homo sapiens
118 KPNA3 3839
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
119 TOR1A 1861
Affinity Capture-MS Homo sapiens
120 NOS1AP  
Affinity Capture-MS Homo sapiens
121 NOVA2  
Affinity Capture-MS Homo sapiens
122 CTBP2 1488
Affinity Capture-MS Homo sapiens
123 TAF4B  
Affinity Capture-MS Homo sapiens
124 POGZ 23126
Affinity Capture-MS Homo sapiens
125 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
126 RASAL3 64926
Affinity Capture-MS Homo sapiens
127 NOP56 10528
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 ARL6IP4  
Affinity Capture-MS Homo sapiens
129 RSBN1  
Affinity Capture-MS Homo sapiens
130 PWP2 5822
Affinity Capture-MS Homo sapiens
131 ETNK1  
Affinity Capture-MS Homo sapiens
132 PSIP1 11168
Affinity Capture-MS Homo sapiens
133 VANGL2  
Affinity Capture-MS Homo sapiens
134 XRCC1 7515
Affinity Capture-MS Homo sapiens
135 RNPS1 10921
Affinity Capture-MS Homo sapiens
136 NOLC1 9221
Affinity Capture-MS Homo sapiens
137 ANKRD2  
Affinity Capture-MS Homo sapiens
138 NPM1 4869
Affinity Capture-MS Homo sapiens
139 SRRM2 23524
Affinity Capture-MS Homo sapiens
140 HIST4H4 121504
Affinity Capture-MS Homo sapiens
141 FIBP 9158
Affinity Capture-MS Homo sapiens
142 C9orf142  
Affinity Capture-MS Homo sapiens
143 SETD5 55209
Affinity Capture-MS Homo sapiens
144 WIZ 58525
Affinity Capture-MS Homo sapiens
145 MED4 29079
Affinity Capture-MS Homo sapiens
146 IPO11 51194
Affinity Capture-MS Homo sapiens
147 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
149 NFRKB  
Affinity Capture-MS Homo sapiens
150 HIST1H4E 8367
Affinity Capture-MS Homo sapiens
151 Spop  
Biochemical Activity Mus musculus
152 TBR1  
Affinity Capture-MS Homo sapiens
153 ACIN1 22985
Affinity Capture-MS Homo sapiens
154 VRK1 7443
Affinity Capture-MS Homo sapiens
155 TAF11  
Affinity Capture-MS Homo sapiens
156 BCL7C  
Affinity Capture-MS Homo sapiens
157 HDGFRP3 50810
Affinity Capture-MS Homo sapiens
158 TNPO3 23534
Affinity Capture-MS Homo sapiens
159 BPTF 2186
Affinity Capture-MS Homo sapiens
160 TCTN2  
Affinity Capture-MS Homo sapiens
161 LUC7L2 51631
Affinity Capture-MS Homo sapiens
162 RCC1 1104
Affinity Capture-MS Homo sapiens
163 ABHD8  
Affinity Capture-MS Homo sapiens
164 UBTF 7343
Affinity Capture-MS Homo sapiens
165 LYAR 55646
Affinity Capture-MS Homo sapiens
166 SPDYE4  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
167 POLB 5423
Affinity Capture-MS Homo sapiens
168 SS18L1  
Affinity Capture-MS Homo sapiens
169 HMGN3  
Affinity Capture-MS Homo sapiens
170 YEATS4  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
171 TPX2  
Affinity Capture-MS Homo sapiens
172 SMCHD1 23347
Affinity Capture-MS Homo sapiens
173 SCML1  
Affinity Capture-MS Homo sapiens
174 PHIP 55023
Affinity Capture-MS Homo sapiens
175 ATP5F1 515
Affinity Capture-MS Homo sapiens
176 DACH1  
Affinity Capture-MS Homo sapiens
177 LTBP4 8425
Affinity Capture-MS Homo sapiens
178 EGFR 1956
Co-localization Homo sapiens
179 FAM90A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
180 MORF4L1  
Affinity Capture-MS Homo sapiens
181 DMAP1 55929
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
182 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
183 C1orf174  
Affinity Capture-MS Homo sapiens
184 MYC  
Reconstituted Complex Homo sapiens
185 SET 6418
Affinity Capture-MS Homo sapiens
186 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
187 EHMT2 10919
Affinity Capture-MS Homo sapiens
188 NIPBL 25836
Affinity Capture-MS Homo sapiens
189 HIST1H4F 8361
Affinity Capture-MS Homo sapiens
190 RCN2 5955
Affinity Capture-MS Homo sapiens
191 Junb  
Affinity Capture-MS Mus musculus
192 ARGLU1  
Affinity Capture-MS Homo sapiens
193 HMG20A  
Affinity Capture-MS Homo sapiens
194 BRD9 65980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
195 FTSJ3 117246
Affinity Capture-MS Homo sapiens
196 TAF4 6874
Affinity Capture-MS Homo sapiens
197 NKAP 79576
Affinity Capture-MS Homo sapiens
198 MED11  
Affinity Capture-MS Homo sapiens
199 GATAD2A 54815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
200 SLC4A1 6521
Affinity Capture-MS Homo sapiens
201 TAF6 6878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
202 SURF6  
Affinity Capture-MS Homo sapiens
203 GOT2 2806
Affinity Capture-MS Homo sapiens
204 SPOP  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
205 TERF2  
Affinity Capture-MS Homo sapiens
206 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
207 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
208 SPP1 6696
Two-hybrid Homo sapiens
209 CALU 813
Affinity Capture-MS Homo sapiens
210 RIF1  
Affinity Capture-MS Homo sapiens
211 KNOP1 400506
Affinity Capture-MS Homo sapiens
212 ATP5L2  
Affinity Capture-MS Homo sapiens
213 C1orf35  
Affinity Capture-MS Homo sapiens
214 TSPYL1 7259
Affinity Capture-MS Homo sapiens
215 CHD1 1105
Affinity Capture-MS Homo sapiens
216 NIFK 84365
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
217 TNNC1  
Affinity Capture-MS Homo sapiens
218 RPS26 6231
Affinity Capture-MS Homo sapiens
219 SFSWAP  
Affinity Capture-MS Homo sapiens
220 DHX38 9785
Affinity Capture-MS Homo sapiens
221 C2CD5 9847
Affinity Capture-MS Homo sapiens
222 MTA3 57504
Affinity Capture-MS Homo sapiens
223 PTRF 284119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
224 BCL7A  
Affinity Capture-MS Homo sapiens
225 CC2D1A 54862
Affinity Capture-MS Homo sapiens
226 ABCA12  
Affinity Capture-MS Homo sapiens
227 CBX1 10951
Affinity Capture-MS Homo sapiens
228 NOP58 51602
Affinity Capture-MS