Gene description for HERC1
Gene name HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Gene symbol HERC1
Other names/aliases p532
p619
Species Homo sapiens
 Database cross references - HERC1
ExoCarta ExoCarta_8925
Entrez Gene 8925
HGNC 4867
MIM 605109
UniProt Q15751  
 HERC1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for HERC1
Molecular Function
    ligase activity GO:0016874 IEA
    ARF guanyl-nucleotide exchange factor activity GO:0005086 TAS
    ubiquitin-protein transferase activity GO:0004842 IEA
Biological Process
    transport GO:0006810 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    cerebellar Purkinje cell differentiation GO:0021702 IEA
    negative regulation of autophagy GO:0010507 IEA
    neuron projection development GO:0031175 IEA
    protein ubiquitination GO:0016567 IEA
    positive regulation of GTPase activity GO:0043547 TAS
Subcellular Localization
    membrane GO:0016020 IEA
    cytosol GO:0005829 IEA
    Golgi apparatus GO:0005794 IEA
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified HERC1 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for HERC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DFFA  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HERC1 is involved
No pathways found





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