Gene description for SYNE2
Gene name spectrin repeat containing, nuclear envelope 2
Gene symbol SYNE2
Other names/aliases EDMD5
NUA
NUANCE
Nesp2
Nesprin-2
SYNE-2
TROPH
Species Homo sapiens
 Database cross references - SYNE2
ExoCarta ExoCarta_23224
Vesiclepedia VP_23224
Entrez Gene 23224
HGNC 17084
MIM 608442
UniProt Q8WXH0  
 SYNE2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Liver cancer cells 25265333    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SYNE2
Molecular Function
    actin binding GO:0003779 ISS
    protein binding GO:0005515 IPI
    cytoskeleton-nuclear membrane anchor activity GO:0140444 IDA
Biological Process
    nuclear migration GO:0007097 IMP
    nucleokinesis involved in cell motility in cerebral cortex radial glia guided migration GO:0021817 IEA
    positive regulation of cell migration GO:0030335 ISS
    nuclear migration along microfilament GO:0031022 ISS
    centrosome localization GO:0051642 IMP
    regulation of cilium assembly GO:1902017 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nuclear envelope GO:0005635 IDA
    nuclear outer membrane GO:0005640 IEA
    nucleoplasm GO:0005654 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    focal adhesion GO:0005925 IDA
    sarcoplasmic reticulum GO:0016529 IDA
    Z disc GO:0030018 IDA
    lamellipodium membrane GO:0031258 IDA
    filopodium membrane GO:0031527 IDA
    nuclear membrane GO:0031965 IDA
    nuclear lumen GO:0031981 IDA
    sarcoplasmic reticulum membrane GO:0033017 IEA
    meiotic nuclear membrane microtubule tethering complex GO:0034993 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SYNE2 in exosomes
1
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 267
MISEV standards
Biophysical techniques
TSG101|Alix
Enriched markers
CANX|HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SYNE2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRP3  
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 RNASEH2B  
Affinity Capture-MS Homo sapiens
4 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 GJA1 2697
Proximity Label-MS Homo sapiens
8 CEP170 9859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NUP62 23636
Affinity Capture-MS Homo sapiens
10 RPL37A 6168
Cross-Linking-MS (XL-MS) Homo sapiens
11 CTDNEP1 23399
Proximity Label-MS Homo sapiens
12 ACTC1 70
Reconstituted Complex Homo sapiens
13 TBC1D16  
Affinity Capture-MS Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 HSD17B11 51170
Proximity Label-MS Homo sapiens
16 UBC 7316
Reconstituted Complex Homo sapiens
17 LAMP2 3920
Proximity Label-MS Homo sapiens
18 DNAJC25 548645
Proximity Label-MS Homo sapiens
19 CALM1 801
Reconstituted Complex Homo sapiens
20 PTPN1 5770
Proximity Label-MS Homo sapiens
21 STX4 6810
Proximity Label-MS Homo sapiens
22 CAPN6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NDUFB9 4715
Co-fractionation Homo sapiens
24 S100A4 6275
Affinity Capture-MS Homo sapiens
25 B3GAT1  
Proximity Label-MS Homo sapiens
26 TFAM 7019
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
27 Cul1 26965
Affinity Capture-MS Mus musculus
28 RAB3B 5865
Proximity Label-MS Homo sapiens
29 STAT3 6774
Affinity Capture-MS Homo sapiens
30 GBP7  
Affinity Capture-MS Homo sapiens
31 SSR1 6745
Proximity Label-MS Homo sapiens
32 MARCH5  
Proximity Label-MS Homo sapiens
33 SUN2 25777
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
34 DNAJC16  
Proximity Label-MS Homo sapiens
35 SLC25A46 91137
Proximity Label-MS Homo sapiens
36 PDE6D 5147
Affinity Capture-MS Homo sapiens
37 RAB35 11021
Proximity Label-MS Homo sapiens
38 SOD1 6647
Affinity Capture-MS Homo sapiens
39 OBSL1 23363
Affinity Capture-MS Homo sapiens
40 MAPK8IP2  
Two-hybrid Homo sapiens
41 LMAN1 3998
Proximity Label-MS Homo sapiens
42 MTNR1B  
Two-hybrid Homo sapiens
43 NUP155 9631
Proximity Label-MS Homo sapiens
44 MAPK1 5594
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
45 PFN1 5216
Proximity Label-MS Homo sapiens
46 RAB5A 5868
Proximity Label-MS Homo sapiens
47 SDHA 6389
Co-fractionation Homo sapiens
48 MAPK3 5595
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
49 EMD 2010
Proximity Label-MS Homo sapiens
50 PARK2  
Affinity Capture-MS Homo sapiens
51 CXADR 1525
Proximity Label-MS Homo sapiens
52 SUPT5H 6829
Affinity Capture-MS Homo sapiens
53 PSMD14 10213
Affinity Capture-MS Homo sapiens
54 RAB4A 5867
Proximity Label-MS Homo sapiens
55 MKRN2 23609
Affinity Capture-RNA Homo sapiens
56 Chmp4c  
Affinity Capture-MS Mus musculus
57 PEX14 5195
Proximity Label-MS Homo sapiens
58 RPN1 6184
Proximity Label-MS Homo sapiens
59 PML 5371
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
60 SLX4IP  
Affinity Capture-MS Homo sapiens
61 BRAP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 ELOVL5 60481
Proximity Label-MS Homo sapiens
63 BIN1 274
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 REEP5 7905
Proximity Label-MS Homo sapiens
65 GAN 8139
Affinity Capture-MS Homo sapiens
66 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 LRRC59 55379
Proximity Label-MS Homo sapiens
68 PANX1 24145
Proximity Label-MS Homo sapiens
69 SIRT7  
Affinity Capture-MS Homo sapiens
70 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
71 HOXB2  
Affinity Capture-MS Homo sapiens
72 BCAP31 10134
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 HSD3B7 80270
Proximity Label-MS Homo sapiens
74 NR3C1 2908
Proximity Label-MS Homo sapiens
75 STIM1 6786
Proximity Label-MS Homo sapiens
76 FKBP8 23770
Proximity Label-MS Homo sapiens
77 RPN2 6185
Proximity Label-MS Homo sapiens
78 NUP43 348995
Proximity Label-MS Homo sapiens
79 SUN1 23353
Affinity Capture-Western Homo sapiens
80 TMEM67 91147
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
81 NRM 11270
Affinity Capture-MS Homo sapiens
82 EFTUD2 9343
Affinity Capture-MS Homo sapiens
83 ATP2A1 487
Proximity Label-MS Homo sapiens
84 FBXO2 26232
Affinity Capture-MS Homo sapiens
85 RAB9A 9367
Proximity Label-MS Homo sapiens
86 NDC80 10403
Proximity Label-MS Homo sapiens
87 CUL7 9820
Affinity Capture-MS Homo sapiens
88 Kctd5  
Affinity Capture-MS Mus musculus
89 CYP2C9  
Proximity Label-MS Homo sapiens
90 ACBD5 91452
Proximity Label-MS Homo sapiens
91 ARRB2 409
Affinity Capture-MS Homo sapiens
92 TERF2  
Affinity Capture-MS Homo sapiens
93 CEP170P1  
Affinity Capture-MS Homo sapiens
94 SRRM1 10250
Cross-Linking-MS (XL-MS) Homo sapiens
95 TP53 7157
Affinity Capture-MS Homo sapiens
96 FRAT2  
Affinity Capture-MS Homo sapiens
97 MKS1 54903
Affinity Capture-Western Homo sapiens
98 ICT1 3396
Co-fractionation Homo sapiens
99 CDC37 11140
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
100 THOP1 7064
Co-fractionation Homo sapiens
101 HDAC1 3065
Affinity Capture-MS Homo sapiens
102 MORC3 23515
Affinity Capture-MS Homo sapiens
103 ERGIC1 57222
Proximity Label-MS Homo sapiens
104 HNRNPK 3190
Cross-Linking-MS (XL-MS) Homo sapiens
105 KIAA1715 80856
Proximity Label-MS Homo sapiens
106 PXMP2  
Proximity Label-MS Homo sapiens
107 ERGIC2 51290
Proximity Label-MS Homo sapiens
108 TERF1 7013
Affinity Capture-MS Homo sapiens
109 SEC61B 10952
Proximity Label-MS Homo sapiens
110 SEC62 7095
Proximity Label-MS Homo sapiens
111 RAB7A 7879
Proximity Label-MS Homo sapiens
112 RAB11A 8766
Proximity Label-MS Homo sapiens
113 CIT 11113
Affinity Capture-MS Homo sapiens
114 PRPF4 9128
Affinity Capture-MS Homo sapiens
115 DNAJC1 64215
Proximity Label-MS Homo sapiens
116 HTR1B  
Affinity Capture-MS Homo sapiens
117 CKAP4 10970
Proximity Label-MS Homo sapiens
118 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SYNE2 is involved
PathwayEvidenceSource
Cell Cycle IEA Reactome
Meiosis IEA Reactome
Meiotic synapsis IEA Reactome
Reproduction IEA Reactome





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