Gene description for CDK5RAP3
Gene name CDK5 regulatory subunit associated protein 3
Gene symbol CDK5RAP3
Other names/aliases C53
HSF-27
IC53
LZAP
MST016
OK/SW-cl.114
PP1553
Species Homo sapiens
 Database cross references - CDK5RAP3
ExoCarta ExoCarta_80279
Vesiclepedia VP_80279
Entrez Gene 80279
HGNC 18673
MIM 608202
UniProt Q96JB5  
 CDK5RAP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CDK5RAP3
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein kinase binding GO:0019901 NAS
    cyclin binding GO:0030332 IPI
    ubiquitin-like protein ligase binding GO:0044389 IDA
    mitogen-activated protein kinase binding GO:0051019 IPI
    NF-kappaB binding GO:0051059 IPI
    MDM2/MDM4 family protein binding GO:0097371 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 ISS
    liver development GO:0001889 ISS
    negative regulation of protein phosphorylation GO:0001933 IMP
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IMP
    regulation of mitotic cell cycle GO:0007346 IBA
    brain development GO:0007420 NAS
    cell population proliferation GO:0008283 IDA
    regulation of phosphatase activity GO:0010921 IMP
    apoptotic nuclear changes GO:0030262 IMP
    endoplasmic reticulum unfolded protein response GO:0030968 IMP
    positive regulation of protein ubiquitination GO:0031398 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    ribosome disassembly GO:0032790 IDA
    response to endoplasmic reticulum stress GO:0034976 ISS
    negative regulation of protein catabolic process GO:0042177 IDA
    negative regulation of MAP kinase activity GO:0043407 IMP
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 IMP
    mitotic G2/M transition checkpoint GO:0044818 IMP
    regulation of neuron differentiation GO:0045664 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    definitive erythrocyte differentiation GO:0060318 ISS
    protein ufmylation GO:0071569 IDA
    protein ufmylation GO:0071569 IDA
    protein ufmylation GO:0071569 IMP
    negative regulation of protein serine/threonine kinase activity GO:0071901 IMP
    rescue of stalled ribosome GO:0072344 IDA
    rescue of stalled ribosome GO:0072344 IMP
    positive regulation of reticulophagy GO:0140501 IDA
    positive regulation of reticulophagy GO:0140501 IMP
    positive regulation of protein localization to nucleus GO:1900182 IDA
    positive regulation of signal transduction by p53 class mediator GO:1901798 IDA
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    microtubule GO:0005874 IDA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified CDK5RAP3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDK5RAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSD17B11 51170
Proximity Label-MS Homo sapiens
2 DGCR14  
Two-hybrid Homo sapiens
3 METTL7A 25840
Proximity Label-MS Homo sapiens
4 GJA1 2697
Proximity Label-MS Homo sapiens
5 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TMED4 222068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TPTE2  
Affinity Capture-MS Homo sapiens
8 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 LYPD6B 130576
Affinity Capture-MS Homo sapiens
10 KHK 3795
Affinity Capture-MS Homo sapiens
11 GJD3  
Proximity Label-MS Homo sapiens
12 UBC 7316
Affinity Capture-MS Homo sapiens
13 DNAJC25 548645
Proximity Label-MS Homo sapiens
14 VHL  
Two-hybrid Homo sapiens
15 PTPN1 5770
Proximity Label-MS Homo sapiens
16 STX4 6810
Proximity Label-MS Homo sapiens
17 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
18 TMED5 50999
Affinity Capture-MS Homo sapiens
19 RPS3 6188
Affinity Capture-MS Homo sapiens
20 DNAJB6 10049
Affinity Capture-MS Homo sapiens
21 C9orf78 51759
Affinity Capture-MS Homo sapiens
22 RPL10A 4736
Affinity Capture-MS Homo sapiens
23 TAX1BP3 30851
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 RDX 5962
Proximity Label-MS Homo sapiens
25 OR6T1  
Affinity Capture-MS Homo sapiens
26 EFNA4  
Affinity Capture-MS Homo sapiens
27 RAB3B 5865
Proximity Label-MS Homo sapiens
28 TMED2 10959
Affinity Capture-MS Homo sapiens
29 STX7 8417
Proximity Label-MS Homo sapiens
30 NPM1 4869
Affinity Capture-MS Homo sapiens
31 SSR1 6745
Proximity Label-MS Homo sapiens
32 PLD2 5338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 XYLT1 64131
Affinity Capture-MS Homo sapiens
34 CRMP1 1400
Two-hybrid Homo sapiens
35 CCDC47 57003
Affinity Capture-MS Homo sapiens
36 ATG16L1 55054
Affinity Capture-MS Homo sapiens
37 MRPS7 51081
Affinity Capture-MS Homo sapiens
38 ARL1 400
Affinity Capture-MS Homo sapiens
39 HSPA8 3312
Affinity Capture-MS Homo sapiens
40 NUP155 9631
Proximity Label-MS Homo sapiens
41 KIAA1715 80856
Proximity Label-MS Homo sapiens
42 PXMP2  
Proximity Label-MS Homo sapiens
43 RAB5A 5868
Proximity Label-MS Homo sapiens
44 LMNB1 4001
Proximity Label-MS Homo sapiens
45 EMD 2010
Proximity Label-MS Homo sapiens
46 FKBP6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 PARK2  
Affinity Capture-MS Homo sapiens
48 CXADR 1525
Proximity Label-MS Homo sapiens
49 RPS10 6204
Co-fractionation Homo sapiens
50 MAPRE1 22919
Affinity Capture-MS Homo sapiens
51 RAB4A 5867
Proximity Label-MS Homo sapiens
52 COMTD1 118881
Affinity Capture-MS Homo sapiens
53 ZNF787  
Affinity Capture-MS Homo sapiens
54 MME 4311
Affinity Capture-MS Homo sapiens
55 IGHA1 3493
Affinity Capture-MS Homo sapiens
56 CDK5R1  
Reconstituted Complex Homo sapiens
57 RPN1 6184
Proximity Label-MS Homo sapiens
58 ELOVL5 60481
Proximity Label-MS Homo sapiens
59 IGKC 3514
Affinity Capture-MS Homo sapiens
60 SEC61B 10952
Proximity Label-MS Homo sapiens
61 TMPRSS13  
Affinity Capture-MS Homo sapiens
62 TMED1 11018
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 REEP5 7905
Proximity Label-MS Homo sapiens
64 DISC1 27185
Two-hybrid Homo sapiens
65 SEC63 11231
Proximity Label-MS Homo sapiens
66 S100P 6286
Affinity Capture-MS Homo sapiens
67 TMED9 54732
Affinity Capture-MS Homo sapiens
68 RPN2 6185
Proximity Label-MS Homo sapiens
69 UFC1 51506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 LRRC59 55379
Proximity Label-MS Homo sapiens
72 PANX1 24145
Proximity Label-MS Homo sapiens
73 CDH4 1002
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
74 TAB1 10454
Two-hybrid Homo sapiens
75 CARTPT  
Affinity Capture-MS Homo sapiens
76 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 BCAP31 10134
Proximity Label-MS Homo sapiens
78 HSD3B7 80270
Proximity Label-MS Homo sapiens
79 STIM1 6786
Proximity Label-MS Homo sapiens
80 B4GALNT3  
Affinity Capture-MS Homo sapiens
81 FKBP8 23770
Proximity Label-MS Homo sapiens
82 UPF3A  
Affinity Capture-MS Homo sapiens
83 KPNA2 3838
Two-hybrid Homo sapiens
84 CD226 10666
Affinity Capture-MS Homo sapiens
85 TRIM44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 ATP2A1 487
Proximity Label-MS Homo sapiens
87 RAB9A 9367
Proximity Label-MS Homo sapiens
88 NCLN 56926
Affinity Capture-MS Homo sapiens
89 HSPA4 3308
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 DDRGK1 65992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
91 GLCE 26035
Affinity Capture-MS Homo sapiens
92 TMEM55A 55529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 UPF2 26019
Affinity Capture-MS Homo sapiens
94 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
95 TSPYL4 23270
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 HUWE1 10075
Affinity Capture-MS Homo sapiens
97 FASN 2194
Negative Genetic Homo sapiens
98 TMED10 10972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 AMTN  
Affinity Capture-MS Homo sapiens
100 TUBG1 7283
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
101 SMNDC1  
Affinity Capture-MS Homo sapiens
102 HSPB1 3315
Two-hybrid Homo sapiens
103 CDK5R1  
Two-hybrid Bos taurus
104 MKI67  
Affinity Capture-MS Homo sapiens
105 UFL1 23376
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 SEC62 7095
Proximity Label-MS Homo sapiens
107 B3GAT1  
Proximity Label-MS Homo sapiens
108 RAB7A 7879
Proximity Label-MS Homo sapiens
109 RAB11A 8766
Proximity Label-MS Homo sapiens
110 DNAJC1 64215
Proximity Label-MS Homo sapiens
111 Ufl1  
Affinity Capture-MS Mus musculus
112 CKAP4 10970
Proximity Label-MS Homo sapiens
113 UFM1 51569
Affinity Capture-MS Homo sapiens
114 CDKN2A 1029
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
115 DERL1 79139
Proximity Label-MS Homo sapiens
116 MRVI1 10335
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CDK5RAP3 is involved
No pathways found





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