Gene description for TBC1D15
Gene name TBC1 domain family, member 15
Gene symbol TBC1D15
Other names/aliases RAB7-GAP
Species Homo sapiens
 Database cross references - TBC1D15
ExoCarta ExoCarta_64786
Vesiclepedia VP_64786
Entrez Gene 64786
HGNC 25694
MIM 612662
UniProt Q8TC07  
 TBC1D15 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for TBC1D15
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISS
    protein binding GO:0005515 IPI
Biological Process
    regulation of GTPase activity GO:0043087 ISS
    regulation of cilium assembly GO:1902017 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified TBC1D15 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for TBC1D15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRINP2  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 C5AR1  
Affinity Capture-MS Homo sapiens
4 CCNH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ATP6V1C1 528
Co-fractionation Homo sapiens
7 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PLSCR4  
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 CALCOCO1 57658
Two-hybrid Homo sapiens
11 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 PTPN1 5770
Affinity Capture-MS Homo sapiens
13 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 VPS26A 9559
Co-fractionation Homo sapiens
15 CAPZB 832
Affinity Capture-MS Homo sapiens
16 MEPCE 56257
Affinity Capture-MS Homo sapiens
17 UXS1 80146
Affinity Capture-MS Homo sapiens
18 FIS1 51024
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 KIF22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 STAT3 6774
Co-fractionation Homo sapiens
21 RAB5B 5869
Two-hybrid Homo sapiens
22 PSG8  
Affinity Capture-MS Homo sapiens
23 MRS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TACSTD2 4070
Affinity Capture-MS Homo sapiens
25 ABHD14A  
Affinity Capture-MS Homo sapiens
26 F2RL1  
Affinity Capture-MS Homo sapiens
27 BET1 10282
Affinity Capture-MS Homo sapiens
28 SLC30A9 10463
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 DNM1 1759
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NPAS1  
Affinity Capture-MS Homo sapiens
31 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GPR17 2840
Affinity Capture-MS Homo sapiens
33 TAX1BP1 8887
Two-hybrid Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 CAMLG 819
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 TBC1D17 79735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 E2F4  
Affinity Capture-MS Homo sapiens
38 RAB5A 5868
Two-hybrid Homo sapiens
39 TOLLIP 54472
Proximity Label-MS Homo sapiens
40 ATF6B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MIB1 57534
Proximity Label-MS Homo sapiens
42 GCGR  
Affinity Capture-MS Homo sapiens
43 TMEM126A 84233
Affinity Capture-MS Homo sapiens
44 PARK2  
Affinity Capture-MS Homo sapiens
45 OPTN 10133
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 COMTD1 118881
Affinity Capture-MS Homo sapiens
47 UBAC2 337867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CYS1 192668
Affinity Capture-MS Homo sapiens
49 UBXN8  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GRAMD4 23151
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 GABARAP 11337
Affinity Capture-MS Homo sapiens
52 S1PR1 1901
Affinity Capture-MS Homo sapiens
53 RPA4  
Proximity Label-MS Homo sapiens
54 CUL2 8453
Affinity Capture-MS Homo sapiens
55 DGCR2 9993
Affinity Capture-MS Homo sapiens
56 LRTM2  
Affinity Capture-MS Homo sapiens
57 FLOT2 2319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DNM1L 10059
Co-fractionation Homo sapiens
59 MLNR  
Affinity Capture-MS Homo sapiens
60 UTP15 84135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 NTRK1 4914
Affinity Capture-MS Homo sapiens
62 VIPR2  
Affinity Capture-MS Homo sapiens
63 TMED9 54732
Affinity Capture-MS Homo sapiens
64 Cdc23  
Affinity Capture-MS Mus musculus
65 CEP63  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 BTNL9  
Affinity Capture-MS Homo sapiens
67 XPO1 7514
Affinity Capture-MS Homo sapiens
68 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CUL3 8452
Affinity Capture-MS Homo sapiens
70 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 METTL21B  
Affinity Capture-MS Homo sapiens
72 ARFIP1 27236
Co-fractionation Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 SOHLH1  
Affinity Capture-MS Homo sapiens
75 GPR45  
Affinity Capture-MS Homo sapiens
76 CLEC4M  
Affinity Capture-MS Homo sapiens
77 PTPRN  
Affinity Capture-MS Homo sapiens
78 TGFA  
Affinity Capture-MS Homo sapiens
79 MYO9A 4649
Affinity Capture-MS Homo sapiens
80 DNAJB13  
Proximity Label-MS Homo sapiens
81 AKAP1 8165
Proximity Label-MS Homo sapiens
82 CCDC8  
Affinity Capture-MS Homo sapiens
83 RPS16 6217
Affinity Capture-MS Homo sapiens
84 GCNT3 9245
Affinity Capture-MS Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 ALDH3A2 224
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 TERF2  
Affinity Capture-MS Homo sapiens
88 DNM2 1785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 HAUS1  
Affinity Capture-MS Homo sapiens
90 NHLRC2 374354
Affinity Capture-MS Homo sapiens
91 DLK1  
Affinity Capture-MS Homo sapiens
92 SHMT2 6472
Co-fractionation Homo sapiens
93 PTGER3  
Affinity Capture-MS Homo sapiens
94 IDE 3416
Co-fractionation Homo sapiens
95 ST3GAL4 6484
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 DNAJC9 23234
Affinity Capture-MS Homo sapiens
97 CCDC121  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 PSMG3 84262
Co-fractionation Homo sapiens
99 RAB7A 7879
Proximity Label-MS Homo sapiens
100 DNM3 26052
Affinity Capture-MS Homo sapiens
101 LPAR2 9170
Affinity Capture-MS Homo sapiens
102 TMC4  
Affinity Capture-MS Homo sapiens
103 FAM189A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 SERBP1 26135
Affinity Capture-MS Homo sapiens
105 SNX2 6643
Co-fractionation Homo sapiens
106 VIPR1  
Affinity Capture-MS Homo sapiens
107 RAB5C 5878
Two-hybrid Homo sapiens
108 C9orf72  
Affinity Capture-MS Homo sapiens
109 FXYD3 5349
Affinity Capture-MS Homo sapiens
110 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 THEM6  
Affinity Capture-MS Homo sapiens
112 NEDD8 4738
Affinity Capture-MS Homo sapiens
113 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TBC1D15 is involved
PathwayEvidenceSource
Membrane Trafficking TAS Reactome
Rab regulation of trafficking TAS Reactome
TBC/RABGAPs TAS Reactome
Vesicle-mediated transport TAS Reactome





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