Gene description for PRMT3
Gene name protein arginine methyltransferase 3
Gene symbol PRMT3
Other names/aliases HRMT1L3
Species Homo sapiens
 Database cross references - PRMT3
ExoCarta ExoCarta_10196
Vesiclepedia VP_10196
Entrez Gene 10196
HGNC 30163
MIM 603190
UniProt O60678  
 PRMT3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PRMT3
Molecular Function
    protein binding GO:0005515 IPI
    methyltransferase activity GO:0008168 IDA
    protein-arginine N-methyltransferase activity GO:0016274 IBA
    protein-arginine N-methyltransferase activity GO:0016274 NAS
    protein-arginine N-methyltransferase activity GO:0016274 TAS
    protein-arginine omega-N monomethyltransferase activity GO:0035241 IEA
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 IEA
    histone methyltransferase activity GO:0042054 IBA
    ribosome binding GO:0043022 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    chromatin remodeling GO:0006338 IBA
    regulation of DNA-templated transcription GO:0006355 IBA
    negative regulation of protein ubiquitination GO:0031397 IDA
    methylation GO:0032259 IEA
    negative regulation of retinoic acid biosynthetic process GO:1900053 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PRMT3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRMT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIH1D2  
Two-hybrid Homo sapiens
2 NCBP1 4686
Co-fractionation Homo sapiens
3 TCEB1 6921
Co-fractionation Homo sapiens
4 PSMD6 9861
Co-fractionation Homo sapiens
5 NAPA 8775
Co-fractionation Homo sapiens
6 USP5 8078
Co-fractionation Homo sapiens
7 C9orf78 51759
Affinity Capture-MS Homo sapiens
8 NUMA1 4926
Co-fractionation Homo sapiens
9 CSE1L 1434
Co-fractionation Homo sapiens
10 CSTA 1475
Affinity Capture-MS Homo sapiens
11 VHL  
Affinity Capture-Western Homo sapiens
12 TROVE2 6738
Co-fractionation Homo sapiens
13 PTPN1 5770
Co-fractionation Homo sapiens
14 GFRA1 2674
Co-fractionation Homo sapiens
15 PARP8  
Affinity Capture-MS Homo sapiens
16 OSBP 5007
Co-fractionation Homo sapiens
17 CAPZB 832
Co-fractionation Homo sapiens
18 VTA1 51534
Co-fractionation Homo sapiens
19 RPS20 6224
Co-fractionation Homo sapiens
20 TRIM28 10155
Co-fractionation Homo sapiens
21 GFPT1 2673
Co-fractionation Homo sapiens
22 HIRIP3  
Co-fractionation Homo sapiens
23 LIG1 3978
Co-fractionation Homo sapiens
24 IMPDH2 3615
Co-fractionation Homo sapiens
25 IPO5 3843
Co-fractionation Homo sapiens
26 HSPH1 10808
Co-fractionation Homo sapiens
27 FBL 2091
Biochemical Activity Homo sapiens
28 NARS 4677
Co-fractionation Homo sapiens
29 XPO7 23039
Co-fractionation Homo sapiens
30 FAF1 11124
Co-fractionation Homo sapiens
31 SEC23A 10484
Co-fractionation Homo sapiens
32 TSHB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TMEM132D  
Two-hybrid Homo sapiens
34 DYNC1H1 1778
Co-fractionation Homo sapiens
35 SAMHD1 25939
Co-fractionation Homo sapiens
36 RASSF2 9770
Two-hybrid Homo sapiens
37 C15orf59  
Affinity Capture-MS Homo sapiens
38 AURKA 6790
Affinity Capture-MS Homo sapiens
39 HIST1H4A 8359
Co-fractionation Homo sapiens
40 CAPZA1 829
Co-fractionation Homo sapiens
41 USP11 8237
Co-fractionation Homo sapiens
42 FKBP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RUFY1 80230
Co-fractionation Homo sapiens
44 PARK2  
Affinity Capture-MS Homo sapiens
45 PSME1 5720
Co-fractionation Homo sapiens
46 PLOD2 5352
Co-fractionation Homo sapiens
47 TUBA1B 10376
Affinity Capture-MS Homo sapiens
48 YAP1 10413
Co-fractionation Homo sapiens
49 VCP 7415
Co-fractionation Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 SAR1B 51128
Affinity Capture-MS Homo sapiens
52 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
53 PPARD  
Two-hybrid Homo sapiens
54 BEND7 222389
Two-hybrid Homo sapiens
55 MEOX2  
Two-hybrid Homo sapiens
56 GTF2IRD2B  
Affinity Capture-MS Homo sapiens
57 DYNLL2 140735
Affinity Capture-MS Homo sapiens
58 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
59 DDX39B 7919
Co-fractionation Homo sapiens
60 DNAJB11 51726
Co-fractionation Homo sapiens
61 SNAP29 9342
Affinity Capture-MS Homo sapiens
62 HNRNPD 3184
Affinity Capture-MS Homo sapiens
63 GMPPB 29925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RPS2 6187
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 XPO1 7514
Affinity Capture-MS Homo sapiens
66 PSMD13 5719
Co-fractionation Homo sapiens
67 SH2D1B  
Affinity Capture-MS Homo sapiens
68 EGFR 1956
Affinity Capture-MS Homo sapiens
69 ARFIP1 27236
Co-fractionation Homo sapiens
70 STAT1 6772
Biochemical Activity Homo sapiens
71 C3orf18  
Affinity Capture-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 GNB2 2783
Affinity Capture-MS Homo sapiens
74 MCMBP 79892
Co-fractionation Homo sapiens
75 PRSS1 5644
Affinity Capture-MS Homo sapiens
76 TSC22D2 9819
Two-hybrid Homo sapiens
77 KLHL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 CTNNA1 1495
Co-fractionation Homo sapiens
79 HSPD1 3329
Co-fractionation Homo sapiens
80 XRCC5 7520
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
81 XXYLT1 152002
Affinity Capture-MS Homo sapiens
82 RELA 5970
Co-fractionation Homo sapiens
83 TAF6 6878
Co-fractionation Homo sapiens
84 STX19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 TP53 7157
Affinity Capture-Western Homo sapiens
87 PDCD2L 84306
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 TSKS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
90 HDAC1 3065
Co-fractionation Homo sapiens
91 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
92 RNF20 56254
Co-fractionation Homo sapiens
93 OTUB1 55611
Co-fractionation Homo sapiens
94 PDCD2 5134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 HNRNPK 3190
Co-fractionation Homo sapiens
96 DCD 117159
Affinity Capture-MS Homo sapiens
97 IDE 3416
Co-fractionation Homo sapiens
98 DHX16 8449
Affinity Capture-MS Homo sapiens
99 EIF4B 1975
Co-fractionation Homo sapiens
100 PSMB7 5695
Co-fractionation Homo sapiens
101 Ranbp2  
Affinity Capture-MS Mus musculus
102 ADRM1 11047
Co-fractionation Homo sapiens
103 PRMT1 3276
Affinity Capture-MS Homo sapiens
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