Gene description for OSBPL9
Gene name oxysterol binding protein-like 9
Gene symbol OSBPL9
Other names/aliases ORP-9
ORP9
Species Homo sapiens
 Database cross references - OSBPL9
ExoCarta ExoCarta_114883
Vesiclepedia VP_114883
Entrez Gene 114883
HGNC 16386
MIM 606737
UniProt Q96SU4  
 OSBPL9 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for OSBPL9
Molecular Function
    protein binding GO:0005515 IPI
    sterol binding GO:0032934 IBA
    sterol transfer activity GO:0120015 TAS
Biological Process
    bile acid biosynthetic process GO:0006699 TAS
    sterol transport GO:0015918 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    late endosome membrane GO:0031902 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified OSBPL9 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for OSBPL9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 REEP5 7905
Proximity Label-MS Homo sapiens
2 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
3 DTNBP1  
Affinity Capture-MS Homo sapiens
4 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RAB35 11021
Proximity Label-MS Homo sapiens
6 TMED10 10972
Affinity Capture-MS Homo sapiens
7 TERF2  
Affinity Capture-MS Homo sapiens
8 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
11 HAUS1  
Affinity Capture-MS Homo sapiens
12 SACM1L 22908
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 METTL7A 25840
Proximity Label-MS Homo sapiens
15 FUZ 80199
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RAB5A 5868
Proximity Label-MS Homo sapiens
17 KCNE3  
Affinity Capture-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 NUP155 9631
Proximity Label-MS Homo sapiens
20 ISCA2 122961
Affinity Capture-MS Homo sapiens
21 RAB9A 9367
Proximity Label-MS Homo sapiens
22 LAMP1 3916
Proximity Label-MS Homo sapiens
23 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
24 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
25 CFAP36 112942
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SSR1 6745
Proximity Label-MS Homo sapiens
27 LAMP2 3920
Proximity Label-MS Homo sapiens
28 USP11 8237
Affinity Capture-MS Homo sapiens
29 CALM1 801
Affinity Capture-MS Homo sapiens
30 PARK2  
Affinity Capture-MS Homo sapiens
31 PTPN1 5770
Proximity Label-MS Homo sapiens
32 COPE 11316
Co-fractionation Homo sapiens
33 HMGN2 3151
Co-fractionation Homo sapiens
34 DDX58 23586
Affinity Capture-RNA Homo sapiens
35 TNRC18  
Cross-Linking-MS (XL-MS) Homo sapiens
36 Cct2 12461
Affinity Capture-MS Mus musculus
37 WRAP73 49856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 OSBPL11 114885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KBTBD6  
Affinity Capture-MS Homo sapiens
40 LAMTOR1 55004
Proximity Label-MS Homo sapiens
41 GCH1  
Affinity Capture-MS Homo sapiens
42 OSBPL10  
Affinity Capture-MS Homo sapiens
43 CHMP4B 128866
Affinity Capture-MS Homo sapiens
44 SEC62 7095
Proximity Label-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 MYL4 4635
Affinity Capture-MS Homo sapiens
47 RAB7A 7879
Proximity Label-MS Homo sapiens
48 RAB11A 8766
Proximity Label-MS Homo sapiens
49 HIST1H2BL 8340
Affinity Capture-MS Homo sapiens
50 RPL8 6132
Cross-Linking-MS (XL-MS) Homo sapiens
51 DNAJA1 3301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
53 RAB5C 5878
Proximity Label-MS Homo sapiens
54 HSPB1 3315
Two-hybrid Homo sapiens
55 CKAP4 10970
Proximity Label-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 ELOVL5 60481
Proximity Label-MS Homo sapiens
58 POLD2 5425
Co-fractionation Homo sapiens
59 SEC61B 10952
Proximity Label-MS Homo sapiens
60 RPN2 6185
Proximity Label-MS Homo sapiens
61 C17orf59 54785
Proximity Label-MS Homo sapiens
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