Gene description for WDR44
Gene name WD repeat domain 44
Gene symbol WDR44
Other names/aliases RAB11BP
RPH11
Species Homo sapiens
 Database cross references - WDR44
ExoCarta ExoCarta_54521
Vesiclepedia VP_54521
Entrez Gene 54521
HGNC 30512
UniProt Q5JSH3  
 WDR44 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for WDR44
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IDA
    molecular sequestering activity GO:0140313 IMP
Biological Process
    regulation of vesicle-mediated transport GO:0060627 IMP
    cytosolic ciliogenesis GO:0061824 IMP
    negative regulation of cilium assembly GO:1902018 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified WDR44 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR44
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1H 51606
Co-fractionation Homo sapiens
2 TMEM52B 120939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MCM3 4172
Co-fractionation Homo sapiens
4 KIF2A 3796
Co-fractionation Homo sapiens
5 ACSF2 80221
Affinity Capture-MS Homo sapiens
6 TP53 7157
Affinity Capture-MS Homo sapiens
7 MCAM 4162
Proximity Label-MS Homo sapiens
8 TGFBR2 7048
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 BTNL9  
Affinity Capture-MS Homo sapiens
10 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 RNPS1 10921
Affinity Capture-MS Homo sapiens
13 LRRC59 55379
Proximity Label-MS Homo sapiens
14 KCNE3  
Affinity Capture-MS Homo sapiens
15 EMC8 10328
Affinity Capture-MS Homo sapiens
16 CRLF2 64109
Affinity Capture-MS Homo sapiens
17 PFN1 5216
Proximity Label-MS Homo sapiens
18 PTCH2  
Affinity Capture-MS Homo sapiens
19 RAB11A 8766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FBXL19  
Affinity Capture-Western Homo sapiens
22 POLD1 5424
Co-fractionation Homo sapiens
23 CX3CL1  
Affinity Capture-MS Homo sapiens
24 LSM4 25804
Co-fractionation Homo sapiens
25 MREG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CSNK2A1 1457
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 RPP30 10556
Affinity Capture-MS Homo sapiens
29 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PTPN1 5770
Proximity Label-MS Homo sapiens
31 STX4 6810
Proximity Label-MS Homo sapiens
32 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TMEM150A  
Affinity Capture-MS Homo sapiens
34 OSBP 5007
Co-fractionation Homo sapiens
35 OR5F1  
Affinity Capture-MS Homo sapiens
36 HEPACAM2  
Affinity Capture-MS Homo sapiens
37 ILF2 3608
Co-fractionation Homo sapiens
38 VSIG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 OSBPL11 114885
Co-fractionation Homo sapiens
40 RAB5A 5868
Proximity Label-MS Homo sapiens
41 ASMTL 8623
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PDXDC1 23042
Co-fractionation Homo sapiens
43 ACTG1 71
Affinity Capture-MS Homo sapiens
44 LSM7  
Co-fractionation Homo sapiens
45 Rab11a 53869
Affinity Capture-MS Mus musculus
46 DHX16 8449
Co-fractionation Homo sapiens
47 C22orf42  
Affinity Capture-MS Homo sapiens
48 USP47 55031
Co-fractionation Homo sapiens
49 PDP1 54704
Affinity Capture-MS Homo sapiens
50 RAB11B 9230
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
52 TRIP10 9322
Co-fractionation Homo sapiens
53 PDHB 5162
Co-fractionation Homo sapiens
54 TMEM74  
Affinity Capture-MS Homo sapiens
55 ACTB 60
Affinity Capture-MS Homo sapiens
56 KRAS 3845
Proximity Label-MS Homo sapiens
57 RHOB 388
Proximity Label-MS Homo sapiens
58 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
59 TMEM174  
Affinity Capture-MS Homo sapiens
60 SLC31A1 1317
Affinity Capture-MS Homo sapiens
61 MSRA 4482
Two-hybrid Homo sapiens
62 SLC35F2 54733
Affinity Capture-MS Homo sapiens
63 SPINT2 10653
Affinity Capture-MS Homo sapiens
64 SEC62 7095
Proximity Label-MS Homo sapiens
65 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SEC61B 10952
Proximity Label-MS Homo sapiens
67 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
68 EP300 2033
Affinity Capture-MS Homo sapiens
69 TEX19  
Affinity Capture-MS Homo sapiens
70 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PRPF39 55015
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which WDR44 is involved
No pathways found





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