Gene description for ATP6AP1
Gene name ATPase, H+ transporting, lysosomal accessory protein 1
Gene symbol ATP6AP1
Other names/aliases 16A
ATP6IP1
ATP6S1
Ac45
CF2
VATPS1
XAP-3
XAP3
Species Homo sapiens
 Database cross references - ATP6AP1
ExoCarta ExoCarta_537
Vesiclepedia VP_537
Entrez Gene 537
HGNC 868
MIM 300197
UniProt Q15904  
 ATP6AP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for ATP6AP1
Molecular Function
    ATPase activator activity GO:0001671 IBA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 ISS
    transporter activator activity GO:0141109 IEA
Biological Process
    intracellular iron ion homeostasis GO:0006879 IMP
    vacuolar acidification GO:0007035 NAS
    lysosomal lumen acidification GO:0007042 NAS
    regulation of cellular pH GO:0030641 IBA
    osteoclast development GO:0036035 ISS
    cellular response to increased oxygen levels GO:0036295 IMP
    endosomal lumen acidification GO:0048388 NAS
    intracellular pH reduction GO:0051452 NAS
    Golgi lumen acidification GO:0061795 NAS
    synaptic vesicle lumen acidification GO:0097401 IEA
    endosome to plasma membrane protein transport GO:0099638 ISS
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    lysosomal membrane GO:0005765 NAS
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 NAS
    endosome membrane GO:0010008 NAS
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 IDA
    proton-transporting two-sector ATPase complex GO:0016469 TAS
    clathrin-coated vesicle membrane GO:0030665 IEA
    synaptic vesicle membrane GO:0030672 IEA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
    proton-transporting V-type ATPase complex GO:0033176 IBA
    proton-transporting V-type ATPase complex GO:0033176 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ATP6AP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
12
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP6AP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INSIG1  
Affinity Capture-MS Homo sapiens
2 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 SEC63 11231
Proximity Label-MS Homo sapiens
6 Tmed2 56334
Affinity Capture-MS Mus musculus
7 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HBA1 3039
Two-hybrid Homo sapiens
9 RHOU 58480
Reconstituted Complex Homo sapiens
10 DNAJC25 548645
Proximity Label-MS Homo sapiens
11 ANG 283
Two-hybrid Homo sapiens
12 CLGN 1047
Affinity Capture-MS Homo sapiens
13 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
14 Spast  
Affinity Capture-MS Mus musculus
15 CANX 821
Affinity Capture-MS Homo sapiens
16 ATP6V1A 523
Affinity Capture-MS Homo sapiens
17 PLD4  
Affinity Capture-MS Homo sapiens
18 CMTM8  
Affinity Capture-MS Homo sapiens
19 INSIG2  
Affinity Capture-MS Homo sapiens
20 KIAA2013 90231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ATP6V1G1 9550
Affinity Capture-MS Homo sapiens
22 Bmpr1a  
Affinity Capture-MS Mus musculus
23 DBN1 1627
Affinity Capture-MS Homo sapiens
24 GOLT1B 51026
Affinity Capture-MS Homo sapiens
25 VAMP7 6845
Affinity Capture-MS Homo sapiens
26 SLC2A1 6513
Affinity Capture-MS Homo sapiens
27 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 COPB2 9276
Affinity Capture-MS Homo sapiens
29 SLC34A2 10568
Affinity Capture-MS Homo sapiens
30 ATP6V1C1 528
Co-fractionation Homo sapiens
31 FKBP8 23770
Proximity Label-MS Homo sapiens
32 ATP6V1G2  
Affinity Capture-MS Homo sapiens
33 FBXO6 26270
Affinity Capture-MS Homo sapiens
34 PTPN1 5770
Proximity Label-MS Homo sapiens
35 SYVN1 84447
Biochemical Activity Homo sapiens
36 NCR3  
Affinity Capture-MS Homo sapiens
37 ATP6V0A4 50617
Affinity Capture-MS Homo sapiens
38 NXF1 10482
Affinity Capture-RNA Homo sapiens
39 SERINC2 347735
Affinity Capture-MS Homo sapiens
40 TCIRG1 10312
Affinity Capture-MS Homo sapiens
41 CERS4  
Affinity Capture-MS Homo sapiens
42 PILRB 29990
Affinity Capture-MS Homo sapiens
43 ATP6V0E1  
Affinity Capture-MS Homo sapiens
44 MAPK6  
Affinity Capture-MS Homo sapiens
45 ATP6V0C 527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MYEF2 50804
Affinity Capture-MS Homo sapiens
47 Kcnk1  
Affinity Capture-MS Mus musculus
48 MGST1 4257
Co-fractionation Homo sapiens
49 MOV10 4343
Affinity Capture-RNA Homo sapiens
50 FIS1 51024
Proximity Label-MS Homo sapiens
51 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
52 OGT 8473
Reconstituted Complex Homo sapiens
53 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
54 GORASP2 26003
Affinity Capture-MS Homo sapiens
55 CYB5R3 1727
Co-fractionation Homo sapiens
56 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MKRN2 23609
Affinity Capture-RNA Homo sapiens
58 HNRNPAB 3182
Co-fractionation Homo sapiens
59 HUWE1 10075
Affinity Capture-MS Homo sapiens
60 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 DNAJC1 64215
Proximity Label-MS Homo sapiens
62 KRAS 3845
Negative Genetic Homo sapiens
63 SLC12A6 9990
Affinity Capture-MS Homo sapiens
64 CTDNEP1 23399
Proximity Label-MS Homo sapiens
65 VDAC3 7419
Co-fractionation Homo sapiens
66 SLC14A1  
Affinity Capture-MS Homo sapiens
67 MTUS2 23281
Affinity Capture-MS Homo sapiens
68 LMNA 4000
Proximity Label-MS Homo sapiens
69 RHOT2 89941
Proximity Label-MS Homo sapiens
70 PTPRO 5800
Affinity Capture-MS Homo sapiens
71 ARRDC5  
Affinity Capture-MS Homo sapiens
72 Chmp4b 75608
Affinity Capture-MS Mus musculus
73 NR1I2  
Two-hybrid Homo sapiens
74 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
75 PARK2  
Affinity Capture-MS Homo sapiens
76 TMEM192 201931
Affinity Capture-MS Homo sapiens
77 RMDN3 55177
Proximity Label-MS Homo sapiens
78 DKKL1  
Affinity Capture-MS Homo sapiens
79 TMEM185A  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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