Gene description for DDX54
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Gene symbol DDX54
Other names/aliases DP97
Species Homo sapiens
 Database cross references - DDX54
ExoCarta ExoCarta_79039
Vesiclepedia VP_79039
Entrez Gene 79039
HGNC 20084
MIM 611665
UniProt Q8TDD1  
 DDX54 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for DDX54
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    transcription corepressor activity GO:0003714 IDA
    RNA binding GO:0003723 HDA
    RNA helicase activity GO:0003724 IDA
    signaling receptor binding GO:0005102 IDA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    nuclear estrogen receptor binding GO:0030331 IDA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    rRNA processing GO:0006364 IBA
    RNA processing GO:0006396 IDA
    RNA metabolic process GO:0016070 IDA
    estrogen receptor signaling pathway GO:0030520 TAS
    negative regulation of DNA-templated transcription GO:0045892 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified DDX54 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DDX54
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 POLR2G 5436
Two-hybrid Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 ZNF668  
Affinity Capture-MS Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 COIL  
Proximity Label-MS Homo sapiens
8 ZNF346  
Affinity Capture-MS Homo sapiens
9 METTL7A 25840
Proximity Label-MS Homo sapiens
10 RAB9A 9367
Proximity Label-MS Homo sapiens
11 RPL30 6156
Affinity Capture-MS Homo sapiens
12 PPIE 10450
Affinity Capture-MS Homo sapiens
13 RIT1 6016
Negative Genetic Homo sapiens
14 YWHAE 7531
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 MYO1F 4542
Affinity Capture-MS Homo sapiens
16 RPL13A 23521
Affinity Capture-MS Homo sapiens
17 RPL19 6143
Affinity Capture-MS Homo sapiens
18 BRCA1 672
Affinity Capture-MS Homo sapiens
19 ZNF638 27332
Affinity Capture-MS Homo sapiens
20 RPS19 6223
Affinity Capture-MS Homo sapiens
21 RPL10 6134
Affinity Capture-MS Homo sapiens
22 RPL15 6138
Affinity Capture-MS Homo sapiens
23 TLX2  
Proximity Label-MS Homo sapiens
24 RPL14 9045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CSNK2A1 1457
Biochemical Activity Homo sapiens
26 DNAJC25 548645
Proximity Label-MS Homo sapiens
27 PGR  
Reconstituted Complex Homo sapiens
28 SEMA4A  
Two-hybrid Homo sapiens
29 FGF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PTPN1 5770
Proximity Label-MS Homo sapiens
31 RBM34  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HIPK1 204851
Affinity Capture-MS Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
34 CENPA  
Proximity Label-MS Homo sapiens
35 GSK3A 2931
Affinity Capture-MS Homo sapiens
36 APEX1 328
Proximity Label-MS Homo sapiens
37 RPS9 6203
Affinity Capture-MS Homo sapiens
38 DDX23 9416
Proximity Label-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 RPL10A 4736
Affinity Capture-MS Homo sapiens
41 WDR48 57599
Affinity Capture-MS Homo sapiens
42 TFAM 7019
Proximity Label-MS Homo sapiens
43 SRP19 6728
Affinity Capture-MS Homo sapiens
44 Nedd4 25489
Reconstituted Complex Rattus norvegicus
45 UBE2O 63893
Affinity Capture-MS Homo sapiens
46 CHCHD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RPN1 6184
Proximity Label-MS Homo sapiens
48 PRC1 9055
Affinity Capture-MS Homo sapiens
49 SUPV3L1 6832
Affinity Capture-MS Homo sapiens
50 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ANOS1 3730
Affinity Capture-MS Homo sapiens
52 RPS8 6202
Affinity Capture-MS Homo sapiens
53 WDR12 55759
Co-fractionation Homo sapiens
54 POLR1E  
Proximity Label-MS Homo sapiens
55 SSR1 6745
Proximity Label-MS Homo sapiens
56 ZNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 DDX58 23586
Affinity Capture-RNA Homo sapiens
58 PHF10  
Affinity Capture-MS Homo sapiens
59 HIBCH 26275
Affinity Capture-MS Homo sapiens
60 MAGED2 10916
Affinity Capture-MS Homo sapiens
61 ADARB1 104
Affinity Capture-MS Homo sapiens
62 PRKRA 8575
Affinity Capture-MS Homo sapiens
63 FAF1 11124
Affinity Capture-MS Homo sapiens
64 SRP68 6730
Affinity Capture-MS Homo sapiens
65 RRP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 LSM14B 149986
Affinity Capture-MS Homo sapiens
67 PANX1 24145
Proximity Label-MS Homo sapiens
68 YY1AP1 55249
Affinity Capture-MS Homo sapiens
69 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 NOP16 51491
Affinity Capture-MS Homo sapiens
71 RAB35 11021
Proximity Label-MS Homo sapiens
72 DHX57 90957
Affinity Capture-MS Homo sapiens
73 MYCN  
Affinity Capture-MS Homo sapiens
74 OASL 8638
Affinity Capture-MS Homo sapiens
75 CSNK2B 1460
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 NEDD4 4734
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
77 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 DYNLT1 6993
Two-hybrid Homo sapiens
79 KIF14 9928
Affinity Capture-MS Homo sapiens
80 CLTCL1 8218
Affinity Capture-MS Homo sapiens
81 LIN28A  
Affinity Capture-MS Homo sapiens
82 CASK 8573
Two-hybrid Homo sapiens
83 MDH2 4191
Proximity Label-MS Homo sapiens
84 NUP155 9631
Proximity Label-MS Homo sapiens
85 ZNF394  
Two-hybrid Homo sapiens
86 DHX40  
Proximity Label-MS Homo sapiens
87 MRPS25 64432
Affinity Capture-MS Homo sapiens
88 LMNB1 4001
Proximity Label-MS Homo sapiens
89 NOP2 4839
Affinity Capture-MS Homo sapiens
90 C6orf203  
Proximity Label-MS Homo sapiens
91 LUC7L 55692
Affinity Capture-MS Homo sapiens
92 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
93 DDX21 9188
Affinity Capture-MS Homo sapiens
94 TSFM 10102
Proximity Label-MS Homo sapiens
95 RPL4 6124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 RBM19 9904
Affinity Capture-MS Homo sapiens
97 DRG1 4733
Affinity Capture-MS Homo sapiens
98 LARP7 51574
Affinity Capture-MS Homo sapiens
99 RBM4B  
Affinity Capture-MS Homo sapiens
100 MGME1  
Affinity Capture-MS Homo sapiens
101 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
102 SRSF6 6431
Affinity Capture-MS Homo sapiens
103 PURG  
Affinity Capture-MS Homo sapiens
104 ZBTB48  
Affinity Capture-MS Homo sapiens
105 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
106 RPL18A 6142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 ZNF579  
Affinity Capture-MS Homo sapiens
108 DDX10  
Co-fractionation Homo sapiens
109 VCP 7415
Affinity Capture-MS Homo sapiens
110 SLX4  
Affinity Capture-MS Homo sapiens
111 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
114 RAD18  
Affinity Capture-MS Homo sapiens
115 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 PARP1 142
Proximity Label-MS Homo sapiens
117 Srp72  
Affinity Capture-MS Mus musculus
118 RPL36 25873
Affinity Capture-MS Homo sapiens
119 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
120 ZFP91 80829
Affinity Capture-MS Homo sapiens
121 Bag2  
Affinity Capture-MS Mus musculus
122 LYAR 55646
Affinity Capture-MS Homo sapiens
123 RPS6 6194
Affinity Capture-MS Homo sapiens
124 HECTD1 25831
Affinity Capture-MS Homo sapiens
125 RNMTL1  
Affinity Capture-MS Homo sapiens
126 MRPL11 65003
Proximity Label-MS Homo sapiens
127 SRPK3  
Affinity Capture-MS Homo sapiens
128 MAGEB2 4113
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
130 KLF12  
Affinity Capture-MS Homo sapiens
131 GLTSCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 MRPL2 51069
Affinity Capture-MS Homo sapiens
133 NOP56 10528
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
134 CD3EAP  
Proximity Label-MS Homo sapiens
135 RNASEH1 246243
Affinity Capture-MS Homo sapiens
136 SCARNA22  
Affinity Capture-RNA Homo sapiens
137 ANLN 54443
Affinity Capture-MS Homo sapiens
138 FGFBP1 9982
Affinity Capture-MS Homo sapiens
139 ATG16L1 55054
Affinity Capture-MS Homo sapiens
140 SEC63 11231
Proximity Label-MS Homo sapiens
141 PABPC5 140886
Affinity Capture-MS Homo sapiens
142 ZBTB2 57621
Affinity Capture-MS Homo sapiens
143 RPL31 6160
Proximity Label-MS Homo sapiens
144 TMED9 54732
Two-hybrid Homo sapiens
145 MRRF  
Proximity Label-MS Homo sapiens
146 RPN2 6185
Proximity Label-MS Homo sapiens
147 ABT1 29777
Affinity Capture-MS Homo sapiens
148 MRPS12  
Proximity Label-MS Homo sapiens
149 RPL7A 6130
Affinity Capture-MS Homo sapiens
150 RPL13 6137
Affinity Capture-MS Homo sapiens
151 EIF6 3692
Proximity Label-MS Homo sapiens
152 RC3H2  
Affinity Capture-MS Homo sapiens
153 YBX1 4904
Affinity Capture-MS Homo sapiens
154 SIRT7  
Affinity Capture-MS Homo sapiens
155 SRSF4 6429
Affinity Capture-MS Homo sapiens
156 SRSF1 6426
Affinity Capture-MS Homo sapiens
157 NPM3 10360
Affinity Capture-MS Homo sapiens
158 RPL3 6122
Affinity Capture-MS Homo sapiens
159 CUL3 8452
Affinity Capture-MS Homo sapiens
160 NR3C1 2908
Reconstituted Complex Homo sapiens
161 SRSF5 6430
Affinity Capture-MS Homo sapiens
162 RPL11 6135
Affinity Capture-MS Homo sapiens
163 SSB 6741
Co-fractionation Homo sapiens
164 ARF6 382
Proximity Label-MS Homo sapiens
165 RBM27  
Affinity Capture-MS Homo sapiens
166 RPL35 11224
Affinity Capture-MS Homo sapiens
167 PDCD11 22984
Affinity Capture-MS Homo sapiens
168 RIPK4  
Affinity Capture-MS Homo sapiens
169 RRP15  
Co-fractionation Homo sapiens
170 ZC3H10  
Affinity Capture-MS Homo sapiens
171 DVL3 1857
Two-hybrid Homo sapiens
172 ZNF512  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 C12orf65  
Proximity Label-MS Homo sapiens
174 ESR2  
Reconstituted Complex Homo sapiens
175 CBX6  
Affinity Capture-MS Homo sapiens
176 RNF220  
Affinity Capture-MS Homo sapiens
177 MYC  
Affinity Capture-MS Homo sapiens
178 RPA3 6119
Proximity Label-MS Homo sapiens
179 ZNF692  
Affinity Capture-MS Homo sapiens
180 PRRC2A 7916
Affinity Capture-MS Homo sapiens
181 NOL12 79159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
182 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
183 RPS14 6208
Affinity Capture-MS Homo sapiens
184 SIRT6  
Affinity Capture-MS Homo sapiens
185 ACAD9 28976
Proximity Label-MS Homo sapiens
186 DNAJC1 64215
Proximity Label-MS Homo sapiens
187 RPL18 6141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
188 DDRGK1 65992
Affinity Capture-MS Homo sapiens
189 CACTIN  
Affinity Capture-MS Homo sapiens
190 EIF2B1 1967
Affinity Capture-MS Homo sapiens
191 H2AFB2  
Affinity Capture-MS Homo sapiens
192 MRPS17 51373
Affinity Capture-MS Homo sapiens
193 RPS10 6204
Affinity Capture-MS Homo sapiens
194 HIST1H1T 3010
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
195 BMP4 652
Affinity Capture-MS Homo sapiens
196 ZC3H3  
Affinity Capture-MS Homo sapiens
197 NEDD4L 23327
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
198 MINA 84864
Affinity Capture-MS Homo sapiens
199 CRNN 49860
Affinity Capture-MS Homo sapiens
200 LBR 3930
Affinity Capture-MS Homo sapiens
201 CLPP 8192
Proximity Label-MS Homo sapiens
202 DDX50 79009
Affinity Capture-MS Homo sapiens
203 SURF6  
Affinity Capture-MS Homo sapiens
204 CLPX 10845
Affinity Capture-MS Homo sapiens
205 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
206 FASN 2194
Negative Genetic Homo sapiens
207 GRPEL2  
Affinity Capture-MS Homo sapiens
208 RC3H1 149041
Affinity Capture-MS Homo sapiens
209 AUH 549
Proximity Label-MS Homo sapiens
210 TP53 7157
Affinity Capture-MS Homo sapiens
211 TRPM3  
Affinity Capture-MS Homo sapiens
212 PDGFB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
213 ILF3 3609
Affinity Capture-MS Homo sapiens
214 RPLP0 6175
Affinity Capture-MS Homo sapiens
215 PTPRD 5789
Reconstituted Complex Homo sapiens
216 ICT1 3396
Proximity Label-MS Homo sapiens
217 MNDA 4332
Affinity Capture-MS Homo sapiens
218 ZNF746  
Affinity Capture-MS Homo sapiens
219 RNF151  
Affinity Capture-MS Homo sapiens
220 USP36  
Affinity Capture-MS Homo sapiens
221 TPTE  
Proximity Label-MS Homo sapiens
222 MEN1 4221
Affinity Capture-MS Homo sapiens
223 TPT1 7178
Affinity Capture-MS Homo sapiens
224 DGCR8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
225 LCK 3932
Proximity Label-MS Homo sapiens
226 SMURF1 57154
Biochemical Activity Homo sapiens
227 ZNF48  
Affinity Capture-MS Homo sapiens
228 RBM23  
Affinity Capture-MS Homo sapiens
229 SRP72 6731
Co-fractionation Homo sapiens
230 COX8A  
Proximity Label-MS Homo sapiens
231 OTUB1 55611
Affinity Capture-MS Homo sapiens
232 H2AFB3  
Affinity Capture-MS Homo sapiens
233 FAM83H 286077
Affinity Capture-MS Homo sapiens
234 C21orf33